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Mucus Hydration in Subjects with Stable Chronic Bronchitis: A Comparison of Spontaneous and Induced Sputum.
Henderson, Ashley G; Anderson, Wayne H; Ceppe, Agathe; Coakley, Raymond D; Button, Brian; Alexis, Neil E; Peden, David B; Lazarowski, Eduardo R; Davis, C W; Fuller, Fred; Almond, Martha; Qaqish, Bahjat; Kesimer, Mehmet; Boucher, Richard C.
Afiliação
  • Henderson AG; a Pulmonary and Critical Care Medicine, Department of Medicine , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Anderson WH; b Pulmonary and Critical Care Medicine, Department of Medicine and Marsico Lung Institute , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Ceppe A; a Pulmonary and Critical Care Medicine, Department of Medicine , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Coakley RD; a Pulmonary and Critical Care Medicine, Department of Medicine , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Button B; c Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Alexis NE; d Marsico Lung Institute, Center for Environmental Medicine, Asthma and Lung Biology , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Peden DB; d Marsico Lung Institute, Center for Environmental Medicine, Asthma and Lung Biology , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Lazarowski ER; c Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Davis CW; c Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Fuller F; e Division of Urology , NC Memorial Hospital, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Almond M; a Pulmonary and Critical Care Medicine, Department of Medicine , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Qaqish B; f Department of Statistics , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Kesimer M; c Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
  • Boucher RC; c Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
COPD ; 15(6): 572-580, 2018 12.
Article em En | MEDLINE | ID: mdl-30712400
Mucus hydration is important in mucus clearance and lung health. This study sought to test the relative utility of spontaneous sputum (SS) versus the reasonably noninvasive induced sputum (IS) samples for measurement of mucus hydration. SS and IS samples were collected over a 2-day study interval. Sputum was induced with escalating inhaled nebulized 3-5% hypertonic saline. Viscous portions of the samples ("plugs") were utilized for percent solids and total mucin analyses. Cytokines, nucleotides/nucleosides and cell differentials were measured in plugs diluted into 0.1% Sputolysin. Overall, 61.5% of chronic bronchitis (CB) subjects produced a SS sample and 95.2% an IS sample. Total expectorate sample weights were less for the SS (0.94 ± 0.98 g) than the IS (2.67 ± 2.33 g) samples. Percent solids for the SS samples (3.56% ± 1.95; n = 162) were significantly greater than the IS samples (3.08% ± 1.81; n = 121), p = 0.133. Total mucin concentrations also exhibited a dilution of the IS samples: SS = 4.15 ± 3.23 mg/ml (n = 62) versus IS= 3.34 ± 2.55 mg/ml (n = 71) (p = 0.371). Total mucins (combined SS and IS) but not percent solids, were inversely associated with FEV1 percent predicted (p = 0.052) and FEV1,/FVC % (p = 0.035). There were no significant differences between sample types in cytokine or differential cell counts. The probability of sample collections was less for SS than IS samples. Measurements of hydration revealed modest dilution of the IS samples compared to SS. Thus for measurements of mucus hydration, both SS and IS samples appear to be largely interchangeable.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Bronquite Crônica / Mucinas / Muco Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: COPD Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Bronquite Crônica / Mucinas / Muco Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: COPD Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos