A circular RNA derived from COL6A3 functions as a ceRNA in gastric cancer development.
Biochem Biophys Res Commun
; 515(1): 16-23, 2019 07 12.
Article
em En
| MEDLINE
| ID: mdl-31122696
ABSTRACT
Gastric cancer (GC) poses a serious threat to human life, whereas its pathogenesis remains elusive. The present study aimed to investigate the potential pathogenic mechanism behind gastric cancer development. RT-PCR analysis using divergent primers, RNase R digestion assay, and mRNA stability assay were performed to characterize circCOL6A3 (ID hsa_circ_0006401) in GC cell lines; Western blot was conducted to detect the expression of COL6A3. Cell counting kit-8 (CCK-8), EdU incorporation assay, and Transwell were run to evaluate GC cell proliferation and migration. Luciferase reporter assay was performed to validate the relationship between miR-3064-5p and COL6A3. Both circCOL6A3 and COL6A3 were highly expressed in GC cells, while miR-3064-5p was down-regulated. Depleted circCOL6A3 significantly decreased cell viability and mobility, and increased cell apoptosis. CircCOL6A3 regulated the expression of miR-3064-5p, and the effect of si-circCOL6A3 on cell biological behaviors was abolished by miR-3064-5p inhibitor. MicroRNA-3064-5p targets COL6A3 to regulate its expression. Taken together, the present study indicated that overexpressed circCOL6A3 promoted cell proliferation, migration and apoptosis of gastric cancer through rescission of miR-3064-5p-induced inhibitory effect on COL6A3. Our study will furnish theoretical grounds for future clinical diagnosis and treatment of GC patients.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Colágeno Tipo VI
/
RNA Circular
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China