Theragnosis by a miR-141-3p molecular beacon: simultaneous detection and sensitization of 5-fluorouracil resistant colorectal cancer cells through the activation of the TRIM13-associated apoptotic pathway.

Moon, Sung Ung; Park, Yongkeun; Park, Min Geun; Song, Sung Kyu; Jeong, Seok Hoo; Lee, Yong Seung; Heo, Hye Jung; Jung, Woon Yong; Kim, Soonhag

Moon, Sung Ung; Park, Yongkeun; Park, Min Geun; Song, Sung Kyu; Jeong, Seok Hoo; Lee, Yong Seung; Heo, Hye Jung; Jung, Woon Yong; Kim, Soonhag.

Afiliação

Moon SU; Center for Advanced Bioinformatics & Systems Medicine, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-gu, Seoul, 140-742, Republic of Korea.
Park Y; Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, 25 Simgok-ro 100 gil Seo-Gu, Incheon Metropolitan City, 22711, Republic of Korea.
Park MG; Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, 25 Simgok-ro 100 gil Seo-Gu, Incheon Metropolitan City, 22711, Republic of Korea.
Song SK; Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, 25 Simgok-ro 100 gil Seo-Gu, Incheon Metropolitan City, 22711, Republic of Korea.
Jeong SH; Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St. Mary's Hospital, 25 Simgok-ro 100 gil Seo-Gu, Incheon Metropolitan City, 22711, Republic of Korea.
Lee YS; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, 24 Beomil-ro 579 gil, Gangneung-si, Gangwon-do 25601, Republic of Korea. kimsoonhag@empas.com.
Heo HJ; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, 24 Beomil-ro 579 gil, Gangneung-si, Gangwon-do 25601, Republic of Korea. kimsoonhag@empas.com.
Jung WY; Department of Pathology, Hanyang University Guri Hospital, Hanyang University College of Medicine, Kyoungchun-ro 153, Guri-si, Gyeonggi-do 11923, Republic of Korea. pathjwy@gmail.com.
Kim S; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, 24 Beomil-ro 579 gil, Gangneung-si, Gangwon-do 25601, Republic of Korea. kimsoonhag@empas.com.

Chem Commun (Camb) ; 55(52): 7466-7469, 2019 Jul 04.

Article em En | MEDLINE | ID: mdl-31184647

RESUMO

We developed a molecular beacon targeting miR-141-3p, aberrantly increased in 5-fluorouracil-resistant colorectal cancer cells (R-CRCCs). It consists of a fluorophore-labeled oligonucleotide, antisense to miR-141-3p, and a quencher. It detected R-CRCCs and recovered the chemosensitivity of them to 5-fluorouracil by hybridization with miR-141-3p, which is applicable to cancer treatment.

Assuntos

Apoptose/efeitos dos fármacos; Proteínas de Ligação a DNA/metabolismo; Fluoruracila/farmacologia; MicroRNAs/metabolismo; Proteínas Supressoras de Tumor/metabolismo; Antagomirs/metabolismo; Linhagem Celular Tumoral; Neoplasias Colorretais/metabolismo; Neoplasias Colorretais/patologia; Proteínas de Ligação a DNA/química; Proteínas de Ligação a DNA/genética; Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos; Recuperação de Fluorescência Após Fotodegradação; Humanos; MicroRNAs/antagonistas & inibidores; MicroRNAs/genética; Microscopia Confocal; Hibridização de Ácido Nucleico; Fator de Crescimento Transformador beta/metabolismo; Proteínas Supressoras de Tumor/química; Proteínas Supressoras de Tumor/genética

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Proteínas Supressoras de Tumor / MicroRNAs / Proteínas de Ligação a DNA / Fluoruracila Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2019 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google