A simple highly specific fluorescent probe for simultaneous discrimination of cysteine/homocysteine and glutathione/hydrogen sulfide in living cells and zebrafish using two separated fluorescence channels under single wavelength excitation.
Analyst
; 144(14): 4258-4265, 2019 Jul 21.
Article
em En
| MEDLINE
| ID: mdl-31215916
ABSTRACT
Biothiols such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H2S) are widely found in mammalian cells. They are closely related to the production and metabolic pathways and play very important roles in physiological and pathological activities. Therefore, the quantitative detection of these biothiols is of great significance. Although many fluorescent probes have been successfully used to track biothiols in biological samples, the fluorescence method for simultaneously detecting these biothiols using separated fluorescence emission channels under single wavelength excitation is still immature. In this work, we prepared the conjugate of seminaphthorhodafluor (SNARF) dye and 7-nitro-1,2,3-benzoxadiazole (NBD) using as a simple long-wavelength fluorescent probe SNARF-NBD for specific detection of biothiols. Cys/Hcy and GSH/H2S were identified by two separated fluorescence emission channels under single wavelength excitation, which showed good selectivity and sensitivity. In addition, SNARF-NBD has low cytotoxicity and shows good imaging ability in living cells and zebrafish.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cisteína
/
Corantes Fluorescentes
/
Glutationa
/
Homocisteína
/
Sulfeto de Hidrogênio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Analyst
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China