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Lactate production is a prioritized feature of adipocyte metabolism.
Krycer, James R; Quek, Lake-Ee; Francis, Deanne; Fazakerley, Daniel J; Elkington, Sarah D; Diaz-Vegas, Alexis; Cooke, Kristen C; Weiss, Fiona C; Duan, Xiaowen; Kurdyukov, Sergey; Zhou, Ping-Xin; Tambar, Uttam K; Hirayama, Akiyoshi; Ikeda, Satsuki; Kamei, Yushi; Soga, Tomoyoshi; Cooney, Gregory J; James, David E.
Afiliação
  • Krycer JR; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Quek LE; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia; School of Mathematics and Statistics, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Francis D; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Fazakerley DJ; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia; Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Sci
  • Elkington SD; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Diaz-Vegas A; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Cooke KC; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Weiss FC; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Duan X; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Kurdyukov S; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia.
  • Zhou PX; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9038; School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China.
  • Tambar UK; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9038.
  • Hirayama A; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; AMED-CREST, Japan Agency for Medical Research and Development (AMED), 1-7-1 Otemachi, Chiyoda-Ku, Tokyo 100-0004, Japan.
  • Ikeda S; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
  • Kamei Y; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
  • Soga T; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; AMED-CREST, Japan Agency for Medical Research and Development (AMED), 1-7-1 Otemachi, Chiyoda-Ku, Tokyo 100-0004, Japan.
  • Cooney GJ; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia; Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia. Electronic address: gregory.cooney@sydney.edu.au.
  • James DE; School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2006, Australia; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales 2006, Australia; Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia. Electroni
J Biol Chem ; 295(1): 83-98, 2020 01 03.
Article em En | MEDLINE | ID: mdl-31690627
Adipose tissue is essential for whole-body glucose homeostasis, with a primary role in lipid storage. It has been previously observed that lactate production is also an important metabolic feature of adipocytes, but its relationship to adipose and whole-body glucose disposal remains unclear. Therefore, using a combination of metabolic labeling techniques, here we closely examined lactate production of cultured and primary mammalian adipocytes. Insulin treatment increased glucose uptake and conversion to lactate, with the latter responding more to insulin than did other metabolic fates of glucose. However, lactate production did not just serve as a mechanism to dispose of excess glucose, because we also observed that lactate production in adipocytes did not solely depend on glucose availability and even occurred independently of glucose metabolism. This suggests that lactate production is prioritized in adipocytes. Furthermore, knocking down lactate dehydrogenase specifically in the fat body of Drosophila flies lowered circulating lactate and improved whole-body glucose disposal. These results emphasize that lactate production is an additional metabolic role of adipose tissue beyond lipid storage and release.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos / Ácido Láctico / Homeostase Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos / Ácido Láctico / Homeostase Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália