LncRNA SNHG14 contributes to the progression of NSCLC through miR-206/G6PD pathway.
Thorac Cancer
; 11(5): 1202-1210, 2020 05.
Article
em En
| MEDLINE
| ID: mdl-32153123
ABSTRACT
BACKGROUND:
Previous studies have shown that the dysregulation of lncRNAs participates in non-small cell lung cancer (NSCLC) development. The purpose of this study was to research the biological function of lncRNA SNHG14 and its molecular mechanism in NSCLC progression.METHODS:
RT-PCR was applied for investigating the expression of SNHG14, miR-206 and G6PD. The progression of NSCLC was detected by CCK-8, Transwell and western blot assays. The targets of SNHG14 and miR-206 were measured by dual-luciferase reporter assay.RESULTS:
We found a higher expression of SNHG14 in NSCLC and upregulation of SNHG14 contributed to NSCLC cell proliferation, invasion and migration. However, knockdown of SNHG14 showed the opposite effect on the progression of NSCLC. Specifically, SNHG14 negatively regulated miR-206 expression by binding with it directly. Furthermore, G6PD served as the target of miR-206. Rescue experiments showed that SNHG14 promoted G6PD expression by inhibiting miR-206.CONCLUSIONS:
LncRNA SNHG14 contributed to NSCLC progression through miR-206/G6PD axis, providing novel clues for understanding the mechanism of NSCLC.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores Tumorais
/
Regulação Neoplásica da Expressão Gênica
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Carcinoma Pulmonar de Células não Pequenas
/
MicroRNAs
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RNA Longo não Codificante
/
Glucosefosfato Desidrogenase
/
Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Revista:
Thorac Cancer
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China