CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-Hodgkin lymphoma.
Blood Adv
; 4(8): 1624-1627, 2020 04 28.
Article
em En
| MEDLINE
| ID: mdl-32311011
ABSTRACT
Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(-) Hodgkin lymphoma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doença de Hodgkin
/
Infecções por Vírus Epstein-Barr
Limite:
Humans
Idioma:
En
Revista:
Blood Adv
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Moldávia