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Women in sports: The applicability of reference intervals for 6 commercially available testosterone immunoassays (HemSter Study).
Radisic Biljak, Vanja; Duras, Anamarija; Cicak, Helena; Vidranski, Valentina; Vidranski, Tihomir; Peersman, Nele; Vermeersch, Pieter; Ostroski, Ivanka; Ruzic, Lana; Simundic, Ana-Maria.
Afiliação
  • Radisic Biljak V; Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia. Electronic address: vanja.radisic@gmail.com.
  • Duras A; Department of Medical Biochemistry Laboratory, General Hospital "Varazdin", Varazdin, Croatia.
  • Cicak H; Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.
  • Vidranski V; Department of Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Zagreb, Croatia.
  • Vidranski T; Department of Kinesiology, Faculty of Education, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
  • Peersman N; Laboratory Medicine, UZ Leuven, Leuven, Belgium.
  • Vermeersch P; Laboratory Medicine, UZ Leuven, Leuven, Belgium.
  • Ostroski I; Department of Medical Biochemistry Laboratory, General Hospital "Varazdin", Varazdin, Croatia.
  • Ruzic L; Department of Sport and Exercise Medicine, Faculty of Kinesiology, University of Zagreb, Zagreb, Croatia.
  • Simundic AM; Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
Clin Biochem ; 84: 55-62, 2020 Oct.
Article em En | MEDLINE | ID: mdl-32533955
ABSTRACT

BACKGROUND:

Testosterone levels in female athletes are increased due to their physical activity and correlate with their exercise volume. We therefore hypothesized that the reference intervals (RIs) derived from the general population are not applicable for female athletes. The aim of this study was to evaluate the applicability of the given RIs for 6 commercially available testosterone immunoassays in a group of female athletes.

METHODS:

Our study included 121 female athletes from various sporting disciplines (water polo, handball, volleyball, football, and basketball). The physical activity score was assessed by the Short Form of the International Physical Activity Questionnaire. Total testosterone was measured in serum samples by the reference LC-MS/MS method and six different immunoassays (Abbott Architect 2nd Generation Testosterone, Beckman Coulter Access Testosterone, Roche Elecsys Testosterone II, Siemens Atellica® IM Testosterone II (TSTII), Siemens IMMULITE 2000 Total Testosterone, and Snibe MAGLUMI™ Testosterone).

RESULTS:

There were statistically significant differences in age (P = 0.042), weight (P = 0.001), height (P < 0.001), and BMI (P < 0.001) between athletes across different sports. Their quantitative measurements of physical activity and testosterone concentration did not differ significantly between subgroups of various sports, P = 0.167 and P = 0.181, respectively. All immunoassays had a positive absolute and relative bias, in comparison with the LC-MS/MS. The manufacturer's RI was not verified for Abbott Architect, Beckman Coulter Access, and Roche Elecsys Testosterone methods, with the highest percentage of athletes above RI for Beckman Coulter (30%).

CONCLUSIONS:

We demonstrated that the upper reference limit provided was too low for some young female athletes. Clinical laboratories should consider implementation of the new proposed RIs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esportes / Testosterona / Exercício Físico Limite: Adolescent / Adult / Female / Humans Idioma: En Revista: Clin Biochem Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esportes / Testosterona / Exercício Físico Limite: Adolescent / Adult / Female / Humans Idioma: En Revista: Clin Biochem Ano de publicação: 2020 Tipo de documento: Article