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SNHG10 Promotes Cell Proliferation and Migration in Gastric Cancer by Targeting miR-495-3p/CTNNB1 Axis.
Yuan, Xiu; Yang, Tianwen; Xu, Yun; Ou, Shan; Shi, Peng; Cao, Ming; Zuo, Xin; Liu, Qinglan; Yao, Jie.
Afiliação
  • Yuan X; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Yang T; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Xu Y; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Ou S; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Shi P; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Cao M; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Zuo X; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Liu Q; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China.
  • Yao J; Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, 400000, China. jrek06@163.com.
Dig Dis Sci ; 66(8): 2627-2636, 2021 08.
Article em En | MEDLINE | ID: mdl-32920660
ABSTRACT

BACKGROUND:

Long non-coding RNAs have been acknowledged as the crucial regulators in the progression of human cancers, including gastric cancer (GC). Small nucleolar RNA host gene 10 (SNHG10) has been identified as an oncogene in several cancer types. Nonetheless, it is unclear whether SNHG10 exerts functions in GC cells.

AIMS:

The aims of the current study were to explore the function and underlying mechanism of SNHG10 in GC.

METHODS:

The expression levels of SNHG10, miR-495-3p and catenin beta 1 (CTNNB1) were detected by RT-qPCR. Loss-of-function assays, including CCK-8, colony formation assay, flow cytometry analysis and transwell assays, were conducted to verify the effect of SHNG10 on the proliferation, apoptosis, migration and invasion of GC cells. Mechanism experiments were performed to identify the downstream molecular mechanism of SNHG10.

RESULTS:

SNHG10 was expressed at a high level in GC cells. Knockdown of SNHG10 inhibited the proliferation, migration and invasion of GC cells. Silencing of SNHG10 led to the downregulation of core factors of WNT signaling pathway. Knockdown of SNHG10 could decline the expression of CTNNB1 through sequestering miR-495-3p.

CONCLUSIONS:

SNHG10 promotes the procession of GC through targeting miR-495-3p/CTNNB1 and activating WNT signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / MicroRNAs / Proliferação de Células / Beta Catenina / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Dig Dis Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / MicroRNAs / Proliferação de Células / Beta Catenina / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Dig Dis Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China