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Size Matters: An Evaluation of the Molecular Basis of Ontogenetic Modifications in the Composition of Bothrops jararacussu Snake Venom.
Freitas-de-Sousa, Luciana A; Nachtigall, Pedro G; Portes-Junior, José A; Holding, Matthew L; Nystrom, Gunnar S; Ellsworth, Schyler A; Guimarães, Noranathan C; Tioyama, Emilly; Ortiz, Flora; Silva, Bruno R; Kunz, Tobias S; Junqueira-de-Azevedo, Inácio L M; Grazziotin, Felipe G; Rokyta, Darin R; Moura-da-Silva, Ana M.
Afiliação
  • Freitas-de-Sousa LA; Programa de Pós-Graduação em Ciências-Toxinologia, Laboratório de Imunopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Nachtigall PG; Laboratório Especial de Toxinologia Aplicada, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Portes-Junior JA; Laboratório de Coleções Zoológicas, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Holding ML; Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
  • Nystrom GS; Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
  • Ellsworth SA; Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
  • Guimarães NC; Programa de Pós-Graduação em Ciências-Toxinologia, Laboratório de Imunopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Tioyama E; Programa de Pós-Graduação em Ciências-Toxinologia, Laboratório de Imunopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Ortiz F; Laboratório de Coleções Zoológicas, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Silva BR; Laboratório de Coleções Zoológicas, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Kunz TS; Laboratório de Coleções Zoológicas, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Junqueira-de-Azevedo ILM; Laboratório Especial de Toxinologia Aplicada, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Grazziotin FG; Laboratório de Coleções Zoológicas, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Rokyta DR; Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
  • Moura-da-Silva AM; Programa de Pós-Graduação em Ciências-Toxinologia, Laboratório de Imunopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
Toxins (Basel) ; 12(12)2020 12 11.
Article em En | MEDLINE | ID: mdl-33322460
Ontogenetic changes in venom composition have been described in Bothrops snakes, but only a few studies have attempted to identify the targeted paralogues or the molecular mechanisms involved in modifications of gene expression during ontogeny. In this study, we decoded B. jararacussu venom gland transcripts from six specimens of varying sizes and analyzed the variability in the composition of independent venom proteomes from 19 individuals. We identified 125 distinct putative toxin transcripts, and of these, 73 were detected in venom proteomes and only 10 were involved in the ontogenetic changes. Ontogenetic variability was linearly related to snake size and did not correspond to the maturation of the reproductive stage. Changes in the transcriptome were highly predictive of changes in the venom proteome. The basic myotoxic phospholipases A2 (PLA2s) were the most abundant components in larger snakes, while in venoms from smaller snakes, PIII-class SVMPs were the major components. The snake venom metalloproteinases (SVMPs) identified corresponded to novel sequences and conferred higher pro-coagulant and hemorrhagic functions to the venom of small snakes. The mechanisms modulating venom variability are predominantly related to transcriptional events and may consist of an advantage of higher hematotoxicity and more efficient predatory function in the venom from small snakes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bothrops / Venenos de Crotalídeos / Proteômica / Tamanho Corporal / Transcriptoma Limite: Animals Idioma: En Revista: Toxins (Basel) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bothrops / Venenos de Crotalídeos / Proteômica / Tamanho Corporal / Transcriptoma Limite: Animals Idioma: En Revista: Toxins (Basel) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil