Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins.

Caputo, Alessandro T; Eder, Oliver M; Bereznakova, Hana; Pothuis, Heleen; Ardevol, Albert; Newman, Janet; Nuttall, Stewart; Peat, Thomas S; Adams, Timothy E

Caputo, Alessandro T; Eder, Oliver M; Bereznakova, Hana; Pothuis, Heleen; Ardevol, Albert; Newman, Janet; Nuttall, Stewart; Peat, Thomas S; Adams, Timothy E.

Afiliação

Caputo AT; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia. alex.caputo@csiro.au.
Eder OM; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Bereznakova H; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Pothuis H; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Ardevol A; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Newman J; CSIRO Synthetic Biology Future Science Platform, GPO Box 1700, Canberra, ACT, 2601, Australia.
Nuttall S; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Peat TS; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.
Adams TE; Biomedical Manufacturing, Commonwealth Scientific and Industrial Research Organisation, 343 Royal Parade, Parkville, Victoria, 3052, Australia.

Sci Rep ; 11(1): 5247, 2021 03 04.

Article em En | MEDLINE | ID: mdl-33664348

Assuntos

Acetilcoenzima A/química; Acetiltransferases/ultraestrutura; Proteínas Recombinantes/ultraestrutura; Streptomyces/ultraestrutura; Acetilcoenzima A/genética; Acetilação; Acetiltransferases/química; Acetiltransferases/genética; Animais; Domínio Catalítico/genética; Linhagem Celular; Cristalografia por Raios X; Regulação da Expressão Gênica/genética; Células HEK293; Humanos; Mutação/genética; Puromicina/química; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Streptomyces/enzimologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcoenzima A / Acetiltransferases / Streptomyces / Proteínas Recombinantes Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália

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