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Selection of Gut-Resistant Bacteria and Construction of Microbial Consortia for Improving Gluten Digestion under Simulated Gastrointestinal Conditions.
De Angelis, Maria; Siragusa, Sonya; Vacca, Mirco; Di Cagno, Raffaella; Cristofori, Fernanda; Schwarm, Michael; Pelzer, Stefan; Flügel, Monika; Speckmann, Bodo; Francavilla, Ruggiero; Gobbetti, Marco.
Afiliação
  • De Angelis M; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy.
  • Siragusa S; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy.
  • Vacca M; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy.
  • Di Cagno R; Faculty of Science and Technology, Free University of Bozen, 39100 Bolzano, Italy.
  • Cristofori F; Interdisciplinary Department of Medicine-Pediatric Section, University of Bari Aldo Moro, Ospedale Pediatrico Giovanni XXIII, 70125 Bari, Italy.
  • Schwarm M; Evonik Operations GmbH, 63457 Hanau-Wolfgang, Germany.
  • Pelzer S; Evonik Operations GmbH, 63457 Hanau-Wolfgang, Germany.
  • Flügel M; Evonik Operations GmbH, 63457 Hanau-Wolfgang, Germany.
  • Speckmann B; Evonik Operations GmbH, 63457 Hanau-Wolfgang, Germany.
  • Francavilla R; Interdisciplinary Department of Medicine-Pediatric Section, University of Bari Aldo Moro, Ospedale Pediatrico Giovanni XXIII, 70125 Bari, Italy.
  • Gobbetti M; Faculty of Science and Technology, Free University of Bozen, 39100 Bolzano, Italy.
Nutrients ; 13(3)2021 Mar 19.
Article em En | MEDLINE | ID: mdl-33808622
This work aimed to define the microbial consortia that are able to digest gluten into non-toxic and non-immunogenic peptides in the human gastrointestinal tract. METHODS: 131 out of 504 tested Bacillus and lactic acid bacteria, specifically Bacillus (64), lactobacilli (63), Pediococcus (1), and Weissella (3), showed strong gastrointestinal resistance and were selected for their PepN, PepI, PepX, PepO, and PepP activities toward synthetic substrates. Based on multivariate analysis, 24 strains were clearly distinct from the other tested strains based on having the highest enzymatic activities. As estimated by RP-HPLC and nano-ESI-MS/MS, 6 cytoplasmic extracts out of 24 selected strains showed the ability to hydrolyze immunogenic epitopes, specifically 57-68 of α9-gliadin, 62-75 of A-gliadin, 134-153 of γ-gliadin, and 57-89 (33-mer) of α2-gliadin. Live and lysed cells of selected strains were combined into different microbial consortia for hydrolyzing gluten under gastrointestinal conditions. Commercial proteolytic enzymes (Aspergillusoryzae E1, Aspergillusniger E2, Bacillussubtilis Veron HPP, and Veron PS proteases) were also added to each microbial consortium. Consortium activity was evaluated by ELISA tests, RP-HPLC-nano-ESI-MS/MS, and duodenal explants from celiac disease patients. RESULTS: two microbial consortia (Consortium 4: Lactiplantibacillus (Lp.) plantarum DSM33363 and DSM33364, Lacticaseibacillus (Lc.) paracasei DSM33373, Bacillussubtilis DSM33298, and Bacilluspumilus DSM33301; and Consortium 16: Lp. plantarum DSM33363 and DSM33364, Lc. paracasei DSM33373, Limosilactobacillusreuteri DSM33374, Bacillusmegaterium DSM33300, B.pumilus DSM33297 and DSM33355), containing commercial enzymes, were able to hydrolyze gluten to non-toxic and non-immunogenic peptides under gastrointestinal conditions. CONCLUSIONS: the results of this study provide evidence that selected microbial consortia could potentially improve the digestion of gluten in gluten-sensitive patients by hydrolyzing the immunogenic peptides during gastrointestinal digestion.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Trato Gastrointestinal / Digestão / Microbioma Gastrointestinal / Glutens Limite: Humans Idioma: En Revista: Nutrients Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Trato Gastrointestinal / Digestão / Microbioma Gastrointestinal / Glutens Limite: Humans Idioma: En Revista: Nutrients Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália