Nanohybrid electrochemical enzyme sensor for xanthine determination in fish samples.
3 Biotech
; 11(5): 212, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33928000
ABSTRACT
An amperometric biosensor for xanthine was designed, based on covalent immobilization of xanthine oxidase (XO) of Bacillus pumilus RL-2d onto a screen-printed multi-walled carbon nanotubes gold nanoparticle-based electrodes (Nano-Au/c-MWCNT). The carboxyl groups at the electrode surface were activated by the use of 1-Ethyl-3-(3-dimethylaminopropyl carbodiimide) (EDC) and N-hydroxysuccinimide (NHS). The working electrode was then coated with 6 µL of xanthine oxidase (0.273 U/mg protein). The cyclic voltammetry (CV) study was done for the characterization of the sensor using [K3Fe(CN)6] as an artificial electron donor. The sensitivity (S) and the limit of detection (LOD) of the biosensor were 2388.88 µA/cm2/nM (2.388 µA/cm2/µM) and 1.14 nM, respectively. The developed biosensor was used for determination of fish meat freshness.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
3 Biotech
Ano de publicação:
2021
Tipo de documento:
Article