Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP.

Zhang, Yaqin; Chen, Minyan; Liu, Chengrong; Chen, Jiaqi; Luo, Xinyi; Xue, Yingying; Liang, Qiming; Zhou, Li; Tao, Yu; Li, Mingqiang; Wang, Di; Zhou, Jianhua; Wang, Jiasi

Zhang, Yaqin; Chen, Minyan; Liu, Chengrong; Chen, Jiaqi; Luo, Xinyi; Xue, Yingying; Liang, Qiming; Zhou, Li; Tao, Yu; Li, Mingqiang; Wang, Di; Zhou, Jianhua; Wang, Jiasi.

Afiliação

Zhang Y; School of Life Sciences, Jilin University, Changchun, 130012, China.
Chen M; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China.
Liu C; Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Chen J; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China.
Luo X; School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518107, China.
Xue Y; School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518107, China.
Liang Q; Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Zhou L; Research Center of Translational Medicine, Shanghai Children's Hospital, Shanghai, 200025, China.
Tao Y; Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Li M; Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, 510006, China.
Wang D; Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, China.
Zhou J; Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, China.
Wang J; School of Life Sciences, Jilin University, Changchun, 130012, China.

Sens Actuators B Chem ; 345: 130411, 2021 Oct 15.

Article em En | MEDLINE | ID: mdl-34248284

Palavras-chave

AuNP, gold nanoparticle; COVID-19, Corona Virus Disease 2019; CRISPR, clustered regularly interspaced short palindromic repeats; CRISPR/Cas; Coronavirus disease; DMEM, Dulbecco's modified Eagle's medium; FDA, American Food and Drug Administration; Gold nanoparticle; HCRs, hybridization chain reactions; High-throughput on-site detection; LAMP, loop-mediated isothermal amplification; Loop-mediated isothermal amplification; NMPA, the Chinese National Medical Products Administration; POCT, point of care testing; RPA, recombinase polymerase amplification; RT-qPCR, reverse transcription-real time quantitative PCR; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; TCEP, Tris(2-carboxyethyl) phosphine; TEM, transmission electron microscopy

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Sens Actuators B Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google