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sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA.
Li, Jing; Li, Na; Ning, Chengcheng; Guo, Yun; Ji, Chunhui; Zhu, Xiaozhen; Zhang, Xingxing; Meng, Qingling; Shang, Yunxia; Xiao, Chencheng; Xia, Xianzhu; Cai, Xuepeng; Qiao, Jun.
Afiliação
  • Li J; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Li N; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Ning C; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Guo Y; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Ji C; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Zhu X; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Zhang X; Institute of Animal Science and Veterinary Research, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, China.
  • Meng Q; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Shang Y; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Xiao C; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Xia X; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
  • Cai X; State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
  • Qiao J; College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.
FEMS Microbiol Lett ; 368(18)2021 10 04.
Article em En | MEDLINE | ID: mdl-34543394
Small RNAs (sRNAs) are essential virulent regulators in Salmonella typhimurium (STM). To explore the role of sRNA STnc150 in regulating STM virulence, we constructed a STnc150 deletion strain (ΔSTnc150) and its complementary strain (ΔSTnc150/C). Then, we compared their characteristics to their original parent strain experimentally, identified the target genes of STnc150 and determined the expression levels of target genes. The results showed that the ΔSTnc150 strain exhibited delayed biofilm formation, enhanced adhesion to macrophages, significantly reduced LD50, increased liver and spleen viral loads and more vital pathological damaging ability than its parent and complementary strains. Further, bioinformatics combined with the bacterial dual plasmid reporter system confirmed that the bases 72-88 of STnc150 locating at the secondary stem-loop structure of the STnc150 are complementary with the bases 1-19 in the 5'-terminal of fimA mRNA of the type 1 fimbriae subunit. Western blot analysis showed that fimA protein level was increased in STnc150 strain compared with its parent and complementary strains. Together, this study suggested that STnc150 can down-regulate STM fimA expression at the translation level, which provided insights into the regulatory mechanisms of sRNAs in virulence of STM.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Virulência / RNA Bacteriano / Regulação Bacteriana da Expressão Gênica / Proteínas de Fímbrias / Antígenos de Bactérias Tipo de estudo: Prognostic_studies Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Virulência / RNA Bacteriano / Regulação Bacteriana da Expressão Gênica / Proteínas de Fímbrias / Antígenos de Bactérias Tipo de estudo: Prognostic_studies Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China