LDH nanoparticle adjuvant subunit vaccine induces an effective immune response for porcine epidemic diarrhea virus.
Virology
; 565: 58-64, 2022 01 02.
Article
em En
| MEDLINE
| ID: mdl-34739917
ABSTRACT
Porcine Epidemic Diarrhea (PED) is a highly contagious intestinal disease which mostly caused by Porcine Epidemic Diarrhea Virus (PEDV). The PED has caused huge economic losses to the pig industry all over the world and a valid PEDV vaccine is needed to prevent the infection. In this study, we constructed expression plasmid based on the spike (S) gene of the epidemic PEDV strain. The recombinant eukaryotic S (Se) and prokaryotic S (Sp) subunit proteins were expressed and purified as vaccine antigens. We designed a new subunit vaccine based on S proteins, adjuvanted with layered double hydroxide (LDH). The results indicated that the LDH adjuvanted subunit vaccines induced a better immune effect in terms of antibody level and cellular immune response. In conclusion, this study showed a new design of a PEDV subunit vaccine with nanotechnology and demonstrated the potential for its clinical application.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Coronavirus
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Vacinas de Subunidades Antigênicas
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Vírus da Diarreia Epidêmica Suína
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Nanopartículas
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Glicoproteína da Espícula de Coronavírus
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Hidróxidos
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Imunidade
Limite:
Animals
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Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2022
Tipo de documento:
Article