RAGE displays sex-specific differences in obesity-induced adipose tissue insulin resistance.
Biol Sex Differ
; 13(1): 65, 2022 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-36348465
ABSTRACT
BACKGROUND:
The receptor for advanced glycation end products (RAGE) plays an important role in obesity-associated insulin sensitivity. We have also previously reported that RAGE deficiency improved insulin resistance in obesity-induced adipose tissue. The current study was aimed to elucidate the sex-specific mechanism of RAGE deficiency in adipose tissue metabolic regulation and systemic glucose homeostasis.METHODS:
RAGE-deficient (RAGE-/-) mice were fed a high-fat diet (HFD) and subjected to glucose and insulin tolerance tests. Subcutaneous adipose tissue (sAT) was collected, and macrophage polarization was assessed by quantitative real-time PCR. Immunoblotting was performed to evaluate the insulin signaling in adipose tissues.RESULTS:
Under HFD feeding conditions, body weight and adipocyte size of female RAGE deficient (RAGE-/-) were markedly lower than that of male mice. Female RAGE-/- mice showed significantly improved glucose and insulin tolerance compared to male RAGE-/- mice, accompanied with increased M2 macrophages polarization. Expressions of genes involved in anti-oxidant and browning were up-regulated in adipose tissues of female RAGE-/- mice. Moreover, insulin-induced AKT phosphorylation was significantly elevated in adipose tissue in female RAGE-/- mice compared to male RAGE-/- mice.CONCLUSIONS:
Our findings suggest that RAGE-mediated adipose tissue insulin resistance is sex-specific, which is associated with different expression of genes involved in anti-oxidant and browning and insulin-induced AKT phosphorylation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência à Insulina
Limite:
Animals
Idioma:
En
Revista:
Biol Sex Differ
Ano de publicação:
2022
Tipo de documento:
Article