A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening.

Carrington, Blake; Ramanagoudr-Bhojappa, Ramanagouda; Bresciani, Erica; Han, Tae-Un; Sood, Raman

Carrington, Blake; Ramanagoudr-Bhojappa, Ramanagouda; Bresciani, Erica; Han, Tae-Un; Sood, Raman.

Afiliação

Carrington B; Translational and Functional Genomics Branch, Zebrafish Core, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Ramanagoudr-Bhojappa R; Cancer Genetics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Bresciani E; Oncogenesis and Development Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Han TU; Molecular Neurogenetics Section, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Sood R; Translational and Functional Genomics Branch, Zebrafish Core, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA. rsood@mail.nih.gov.

BMC Genomics ; 23(1): 810, 2022 Dec 07.

Article em En | MEDLINE | ID: mdl-36476416

Assuntos

Mutação Puntual; Peixe-Zebra; Humanos; Animais; Peixe-Zebra/genética; Epitopos; Pesquisa

Palavras-chave

CRISPR/Cas9; Epitope tags; Knock-in; Point mutations; Zebrafish; ssODN

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Mutação Puntual Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

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