Diazepam-insensitive GABAA receptors on postnatal spiral ganglion neurones in culture.
Neuroreport
; 8(3): 591-6, 1997 Feb 10.
Article
em En
| MEDLINE
| ID: mdl-9106729
ABSTRACT
Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to gamma-aminobutyric acid (GABA) using patch-clamp techniques. In our recording conditions, GABA elicited inward currents in > 95% of the neurones which reversed around 0 mV. Similar inward currents were measured using isoguvacin, a specific agonist of GABAA receptors. GABA-gated currents were reversibly inhibited by the channel blocker picrotoxin and the GABA competitive antagonist bicuculline. These functional GABAA receptors are characterized by an insensitivity to benzodiazepines and a relatively high sensitivity to beta-carbolines and barbiturates. These results show that the GABAA receptor pharmacological properties of spiral ganglion neurones are close to those of cerebellar granule cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gânglio Espiral da Cóclea
/
Receptores de GABA-A
/
Diazepam
/
Ácido gama-Aminobutírico
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Neuroreport
Assunto da revista:
NEUROLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Bélgica