Oleoylethanolamide Exhibits GPR119-Dependent Inhibition of Osteoclast Function and GPR119-Independent Promotion of Osteoclast Apoptosis.

Oleoylethanolamide (OEA), a bioactive lipid in bone, is known as an endogenous ligand for G protein-coupled receptor 119 (GPR119). Here, we explored the effects of OEA on osteoclast differentiation, function, and survival. While OEA inhibits osteoclast resorptive function by disrupting actin cytoskeleton, it does not affect receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. OEA attenuates osteoclast spreading, blocks actin ring formation, and eventually impairs bone resorption. Mechanistically, OEA inhibits Rac activation in response to macrophage colony-stimulating factor (M-CSF), but not RANKL. Furthermore, the OEA-mediated cytoskeletal disorganization is abrogated by GPR119 knockdown using small hairpin RNA (shRNA), indicating that GPR119 is pivotal for osteoclast cytoskeletal organization. In addition, OEA induces apoptosis in both control and GPR119 shRNAtransduced osteoclasts, suggesting that GPR119 is not required for osteoclast apoptosis. Collectively, our findings reveal that OEA has inhibitory effects on osteoclast function and survival of mature osteoclasts via GPR119-dependent and GPR119-independent pathways, respectively.

Analysis of Computed Tomography Scan After Anterior Odontoid Screw Fixation with the Herbert Screw: Is It Effective to Reduce Fracture Gap?

OBJECTIVE: To assess computed tomography scans to evaluate the extent of reduction of fracture displacement and fracture gap after anterior odontoid screw fixation using the Herbert screw. METHODS: Thirty-seven odontoid fractures were reduced and treated by anterior odontoid screw fixation with the Herbert screw. There were 37 patients whose age ranged from 20 to 79 years. Three-dimensional computed tomography scans were obtained for all patients to assess the screw position, the presence of the penetration of superior cortex of dens, the extent of reduction of fracture displacement, and fracture gap. RESULTS: Mean fracture displacement was 2.6 ± 3.2 mm before surgery; after the operation this value was 1.0 ± 1.5 mm. The difference in fracture gap between the preoperative and the postoperative state was -0.1 ± 1.1 mm, which was not statistically significant (P = 0.667). We achieved cortical purchase in only 16 of 37 patients (43.2%); cortical purchase was not obtained in 21 patients (56.7%) due to the fear of the risk of the damage of neural and vascular structures. Of these 21 patients who had no penetration of the superior cortex of dens, widening of the fracture gap occurred in 12 patients (57%), no change in 6 patients (29%), and there was shortening in 3 patients (14%). However, of the 16 patients with penetration of apical dens tip, we achieved significant reduction of fracture gap (P = 0.002). CONCLUSIONS: To maximize reduction of fracture gap using the Herbert screw, it is essential to penetrate the apical dens tip.