Uso de las células de sarcoma 180 TG para la obtención de fluídos ascíticos hiperinmunes de ratón para virus aftoso. / [Use of sarcoma 180 TG cells for obtaining ascitic fluid from mice hyperimmune to the foot-and-mouth disease virus]

Mice immunized with FMDV C3 Arg 84 antigen were inoculated intraperitoneally with sarcoma 180 TG. The ascitic fluid obtained by ventral puncture contained high titers of antibodies, similar to those obtained from serum, as determined by neutralization and ELISA tests. Ascitic volumes were 10 to 20 times greater than those obtainable with.

Neutralización del virus de la fiebre aftosa O1 Campos por anticuerpos inducidos por un péptido sintético. / [Neutralization of foot-and-mouth disease virus O1 Campos by antibodies induced by a synthetic peptide]

Foot-and-Mouth disease virus (FMDV), contains a positive single-stranded RNA enclosed in a protein capsid. Previous studies have shown that a synthetic peptide located at the carboxyterminal end of VP1 of FMDV strain O1 Kaufbeuren (O1K) at the amino acid positions 144-159, and coupled to KLH was able to elicit high titers of neutralizing antibodies in guinea pigs and protected them against challenge with the homologous virus (8, 15). FMDV strain O1 Campos (O1 Ca) has a similar amino acid sequence to O1K, differing in amino acid 144 (leucin instead valine). We report that the O1K synthetic peptide 144-159 protects adult mice against challenge with FMDV O1Ca when the peptide is coupled to KLH with glutaraldehyde as coupling agent (Table 2) whereas protection is very low when other carrier proteins are used.

Neutralización del virus de la fiebre aftosa O1 Campos por anticuerpos inducidos por un péptido sintético / Neutraliztion of fost-and-mouth diseases virus O1 Campos by antibodies induced by synthetic peptide

Un hexadecapéptido correspondiente a la secuencia 144-159 de la proteína estructural VP1 del virus de la fiebre aftosa (CFA), cepa O1K, fue sintetizado por métodos químicos. El mismo, acoplado covalentemente a distintas proteínas portadoras, demostró su capacidad de inducir anticuerpos neutralizantes del VFA cepa O1 Campos (O1Ca) en ratones adultos. La protección fue del 100% cuando se lo acopló a la hemocianina de un molusco (KLH) y fue inoculado en cantidad de 60 microng, dividida en dos dosis y adyuvada con mezcla oleosa. Las proteínas utilizadas como portadoras: albúmina bovina, purificado proteico de Mycobacterium (PPD), gliadina y células de Brocelas produjeron una respuesta inmune menor que la KLH (AU)

Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa. / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa. / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.