RESUMEN
Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin, allophycocyanin or phycoerithrin (light absorption, fluorescence emission, and electrophoresis of the protein moieties) while cyanobacterial cells (Nostoc sp.) showed evidence of C-phycocyanin and allophycocyanin. The possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed.(AU)
Asunto(s)
Animales , Clorofila/análisis , Malus/crecimiento & desarrollo , Ficobilinas/análisis , Caracoles/crecimiento & desarrollo , Simbiosis , Espectroscopía de Resonancia Magnética , Malus/metabolismo , Ficocianina/metabolismo , Caracoles/metabolismo , Espectrometría de Masa Bombardeada por Átomos VelocesRESUMEN
La cirugía laparoscópica produjo una revolución en los procedimientos quirúrgicos ginecológicos durante la última decáda. Actualmente, diversas patologías ginecológicas son tratadas por cirugía laparoscópica, incluso la oncológica
Asunto(s)
Humanos , Femenino , Laparoscopía/estadística & datos numéricos , Procedimientos Quirúrgicos Ginecológicos/métodos , Patología , GinecologíaRESUMEN
La introducción de la tecnología necesaria para realizar microlaparoscopía ha creado una metodología de dignóstico ginecológico que puede convertirse, en casos seleccionados, en una alternativa a la laparoscopia tradicional
Asunto(s)
Humanos , Femenino , Laparoscopía/métodos , Procedimientos Quirúrgicos GinecológicosRESUMEN
A new species of cyclopoid copepod, Ozmana huarpium, is described as a symbiont to Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae). Rather large numbers (about one hundred copepods per snail)were found, althought there was no evidence of harm to the host. To our knowledge, O. haemophila (symbiont to P. maculata), and the currently described species, O. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. While O. haemophila is restricted to the haemocoel of its host, O. harpium predominate in the penis sheath, the ctenidium and the mantle cavity, figuring in these pallial organs 63-65 por ciento of total mature forms. The sex ratio of the symbiont is skewed to the female side in these organs, specially in male hosts. The hypothesis that a special female tropism for the male host s pallial organs might ensure interindividual transmission of the symbiont was tested, with indications that the symbiont is mainly transmitted during copulation. (AU)