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An experimental model to perform dynamic studies of exocrine pancreatic secretion in mice.

Calvo, E L; Vaccaro, M I; Maillie, A J; Pivetta, O H.
Artículo en Inglés | BINACIS | ID: bin-49679
An experimental model to perform dynamic studies of exocrine pancreatic secretion from mice has been developed. It consists of a microsurgical procedure in anesthetized mice using a stereoscopic microscope. The bile-pancreatic common duct was individualized, isolated and cannulated. A specially calibrated capillary tube was placed in the free end of the cannula and put on a millimetrical rule. Readings of pure pancreatic juice flow rate were performed minute by minute to obtain a flow rate curve. The procedure allowed the discrimination among increasing betanechol doses of 0.1, 0.2, and 0.4 microgram/g body weight.
Biblioteca responsable: AR1.1