[The effectiveness of photodynamic effects on the clinical and functional state of periodontal tissues in periodontitis]. / Effektivnost' fotodinamicheskogo vozdeistviya na kliniko-funktsional'noe sostoyanie tkanei parodonta pri parodontite.

The aim the study. Increasing the effectiveness of treatment of chronic generalized periodontitis of moderate severity according to microhemodynamics and oxygenation in periodontal tissues. MATERIALS AND METHODS: A clinical and functional study and treatment of chronic generalized periodontitis of moderate degree were conducted in 56 people (22 men and 34 women) aged 37 to 55 years. The patients were divided into 2 groups depending on the type of treatment: group 1 (main) - 28 people (11 men and 17 women, the average age of the group was 43.5±2.8 years, photodynamic therapy (PDT) with 1% dimegin gel using an AFS Spectrum LED emitter with a wavelength of 660 nm and an energy density of 95 J/cm2), in group 2 (control) - 28 people. (9 men and 19 women, the average age of the group was 45.0±3.1 years) standard treatment was performed without PDT. The level of tissue blood flow, its activity and vasomotor activity of microvessels, as well as the blood flow bypass index were determined by laser Doppler flowmetry. The study of oxygenation was carried out by optical tissue oximetry with determination of the index of oxygenation, specific oxygen consumption and perfusion oxygen saturation. RESULTS: After PDT, the periodontal tissues showed an increase in blood flow by 65.5% after 3 months, the level of oxygen metabolism increased by 51%, which increased after 6 months. The use of PDT in the complex treatment of chronic generalized periodontitis of moderate severity has an activating effect on the microcirculation system and the level of oxygen metabolism in periodontal tissues both in the near and long-term follow-up periods. CONCLUSIONS: The use of PDT with 1% dimegin gel has an effective effect on the state of microhemodynamics and oxygenation in periodontal tissues.

[Evaluation of the photodynamic effect on the state of microcirculation and oxygenation in periodontal tissues in the treatment of chronic generalized periodontitis]. / Otsenka fotodinamicheskogo vozdeistviya na sostoyanie mikrotsirkulyatsii i oksigenatsii v tkanyakh parodonta pri lechenii khronicheskogo generalizovannogo parodontita.

OBJECTIVE: Increasing the effectiveness of treatment of chronic generalized periodontitis using PDT based on clinical and functional substantiation of the effects of a photosensitizer. MATERIALS AND METHODS: A clinical and functional study and treatment of moderate chronic generalized periodontitis was carried out in 62 people (26 men and 36 women) aged from 35 to 55 years without a somatic model with an orthognathic occlusion diagnosed according to ICD-10 - K05.3. Of these, 2 groups were divided depending on the type of treatment: Group 1 (main) - patients with moderate chronic generalized periodontitis - 32 people. (17 men and 15 women, average age of the group - 43.2±2.2 years); Group 2 (control) - patients with moderate chronic generalized periodontitis - 30 people. (14 men and 16 women, average age of the group - 44.0±3.3 years). Complex treatment consisted of sanitation of the mouth, removal of dental plaque and curettage of periodontal pockets in group 1, followed by PDT with Revixan gel using a special wired aligner REVIXAN DENTAL LED (16 r). The clinical condition of the periodontium was assessed using the Greene Vermillion Hygienic Index (OHI-S), the Mühlleman Bleeding Index (SBI) modified by Cowell, and the periodontal index PI. To study the state of microcirculation in the gum tissue, the laser Doppler flowmetry (LDF) method was used using the LAKK-M device (NPP «Lazma¼, Russia). The state of microcirculation was assessed by the microcirculation index (M), which characterizes the level of tissue blood flow; parameter - «σ¼, which determines the fluctuation of the erythrocyte flow. According to Wavelet analysis of LDF-grams, the shunt index (SH) of blood flow was determined. In the «LDF + spectrometry¼ mode, oxygenation in periodontal tissues was studied using optical tissue oximetry (OTO), based on the results of which the perfusion saturation index (Sm) and the specific oxygen consumption index (U, %) were determined. RESULTS: According to LDF data, after PDT (group 1), normalization of clinical indices and the level of microcirculation in periodontal tissues was established, which was accompanied by an increase in the level of blood flow (M) and its activity (σ), which persisted after 3 and 6 months. after PDT. The perfusion saturation index (Sm) and specific oxygen consumption (U) increased more significantly after PDT, which persisted after 3 and 6 months. In the control group, the dynamics of indicators was less pronounced. CONCLUSION: The use of PDT with Revixan gel normalizes the clinical condition of the periodontium, indicators of microhemodynamics and oxygen metabolism.

[Modern achievements of the functional diagnostics in dentistry]. / Sovremennye dostizheniya funktsional'noi diagnostiki v stomatologii.

The article presents the development of functional research methods in the maxillofacial region, methods of their application and the results of scientific research on the study of the neuromuscular balance of the masticatory muscles with defects and deformations in the maxillofacial region. Methods of blood circulation research (laser doppler flowmetry, ultrasound dopplerography, computer capillaroscopy) have been developed and implemented in the tissues of the maxillofacial region: periodontal, tooth pulp, in the mucous membrane of the alveolar ridge, which allowed us to study the mechanisms of disease pathogenesis and monitor the effectiveness of treatment in the maxillofacial region.

[Rationale for the use of bioactive cements by in vitro simulation of accidental pulp opening]. / Obosnovanie primeneniya bioaktivnykh tsementov dlya sokhraneniya zhiznesposobnosti pul'py pri ee sluchainom vskrytii.

OBJECTIVE: To study the features of reparative dentinogenesis when using biomaterials during accidental opening of the pulp in an experiment. MATERIAL AND METHODS: In an experiment on 3 dwarf pigs of the Svetlogorsk breed a traumatic opening of the tooth cavity was carried out in 18 teeth (6 molars, 6 premolars, 6 incisors), then Biodentin (9 teeth) and Aurosil (9 teeth) were applied on the opened pulp horn. After 14, 30 and 60 days, dwarf pigs were euthanized, and jaw blocks taken. Histological examination of the teeth was carried out using the method of embedding in methyl methacrylate (OsteoBead, Sigma, USA). Thin sections of 35-50 microns were made from the samples and stained using the «Heavenly Trichrome¼ method. Documentation was carried out using a Leica DM100 microscope and an EC3 digital camera in reflected and transmitted light. RESULTS: Histological examination of the thin sections of teeth stained according to the «Heavenly Trichrome¼ method revealed that 2 weeks after the pulp opening and applying of Aurosil and Biodentin, comparable processes occurred in the tooth cavity. Thus, the proliferation of odontoblasts was noted on the lateral surfaces of the chamber. New dentin layers and odontoblasts spread over the lower surface of the material with the formation of dentinal bridges by 4 weeks of observation in both groups. By week 12, there was a decrease in the proliferative activity of odontoblasts and dentin maturation in all cases. At the initial stages of the experiment, some pulp edema and moderate plethora were noted, which was no longer observed at 4 weeks. By 12 weeks, because of the proliferation of odontoblasts, the volume of the tooth cavity was reduced by a third. By the 6th month in the experiment, no significant changes in the structure of dentin and pulp were revealed. CONCLUSION: According to morphological data the studied biomaterials (Aurosil and Biodentin) contribute to dentin regeneration equally.