ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna / Indirect ELISA for the detection of IgG specific to Newcastle disease virus in quail serum

An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100 percent). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation

Genotyping of infectious bursal disease virus strains by restriction fragment length polymorphism analysis of the VP1, VP2, and VP3 genes.

SUMMARY. This study aimed to genotype infectious bursal disease virus (IBDV) isolates from the Minas Gerais state poultry industry. RNA was extracted from bursae obtained from field cases without passage or commercial vaccines. Genetic subtyping of IBDV isolates and vaccine strains was carried out by the reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) analysis. A 588-bp fragment in the VP1 gene, an 847-bp fragment in the VP2 gene, and a 320-bp fragment in the VP3 gene were amplified by PCR and digested with restriction enzymes PstI and ScaI (VP1); BamHI, BstEII, and PstI (VP2); and NcoI, ScaI, and XbaI (VP3). Our work shows that complementing the clinical history of the outbreaks with RT-PCR followed by RFLP analysis using PstI for VP1, BamHI for VP2, and XbaI for VP3 allowed an accurate classification of a causative agent as a very virulent IBDV.

Teor hídrico traqueal como expressäo do potencial patogênico de vírus vacinais da doença de Newcastle e da bronquite infecciosa / Tracheal moisture content as an expression of the pathogenic potential of Newcastle disease and infectious bronchitis vaccinal viruses

Comparou-se o potencial patogênico de amostras vacinais virais da doença de Newcastle (VDN) e da bronquite infecciosa (VBI), através do teor hídrico traqueal (THT) de galinhas livres de anticorpos anti-VDN e anti-VBI, infectadas por via respiratória, com amostras La Sota e VG-GA de VDN e H-52 e H-120 de VBI. Maior THT foi induzido pelas amostras mais patogênicas (La Sota e H-52) do que pelas amostras menos patogênicas (VG-GA e H-120). O método determinou o potencial patogênico das amostras, sem associaçäo bacteriana no processo infeccioso

Cuidados primarios de saude no Distrito Federal. / Primary health care in the Distrito Federal

O presenta trabalho detalha o plano de assistencia a saude no Distrito Federal para o periodo de 1979-1985. A implantacao da rede basica de saude e enfatizada e sao formuladas recomendacoes sobre regionalizacao dos servicos de saude e formacao de recursos humanos basicos para o funcionamento do sistema