Cytotoxicity and apoptosis induction of the marine Conus flavidus venom in HepG2 cancer cell line.

Cancer is still an area of continuous research for finding more effective and selective agents, so our study aimed to explore new anticancer medicines from Cone snails' venoms as marine natural products with promising biological activities. Venoms from seven cone snails collected from two locations on the Red Sea coast (Marsa Alam (Ma) and Hurghada (Hu)) were extracted and subjected to SDS for protein concentrations. The venoms of C. vexillum (Ma), C. vexillum (Hu), and C. flavidus were found to have the highest protein concentrations (2.66, 2.618, and 2.611 mg/mL, respectively). The venom of C. vexillum (Ma) was found to be cytotoxic against the lung cancer cell line A549 (IC50 = 4.511 ± 0.03 µg/mL). On the other hand, the venom of C. flavidus showed a strong cytotoxic effect on both liver and lung cancer cell lines (IC50 = 1.593 ± 0.05 and 7.836 ± 0.4 µg/mL, respectively) when compared to their normal cell lines. Investigating the apoptotic cell death of C. flavidus venom on HepG2 cell lines, it showed total apoptotic cell death by 22.42-fold compared to untreated control and arresting the cell cycle at G2/M phase. Furthermore, its apoptotic cell death in HepG2 cells was confirmed through the upregulation of pro-apoptotic markers and down-regulation of Bcl-2 in both gene and protein expression levels. These findings confirmed the cytotoxic activity of C. flavidus venom through apoptotic cell death in HepG2 cells. So, a detailed study highlighting its structure and molecular target for developing new anticancer agents from natural sources is required.Communicated by Ramaswamy H. Sarma.

Schindleria parva, a new species of Schindler's fish (Teleostei: Schindleriidae: Schindleria) from Jeddah, Saudi Arabia, Red Sea.

Schindleria parva, a new species of the family Schindleriidae, is described from two specimens collected from the central Red Sea of Saudi Arabia. The new species is characterized by lack of pigmentation on the body, possession of an inconspicuous gas bladder and the presence of small teeth on the premaxillae. The holotype is a female of 11 mm standard length (SL) (11.9 mm total length) and the paratype is a male of 9 mm SL. Dorsal fin rays 10 (9) anal fin rays 9 (7). The body depth at pectoral-fin origin 5% (4%) of SL, depth at anal-fin origin 8% (7%) SL, predorsal length 63% (65%) SL, preanal length 72% (72%) SL, the first anal-fin ray situated below the fourth dorsal-fin ray), a total of 23 + 16 myomeres. The female contained a series of 30 rectangular eggs in a single row, whereas the male is characterized by a short rod-like urogenital papilla. Species of the genus Schindleria are likely the smallest marine vertebrates on the planet and S. parva is likely the smallest Schindleria species in the Red Sea. The global diversity of Schindleria is likely underestimated due to the paedomorphic features of this genus. Its fast generation times make it a species-rich genus of high turnover rates, thus potentially highly important for the trophic food webs of coral reefs. Thus, this finding advances knowledge on the biodiversity of the Red Sea, highlights its conservation significance, and contributes towards the understanding of the complexity of the coral-reef fish community.

Metals in a northern Nile Delta lake: water, suspended particulates, sediments, and biota.

Lake Edku is one of the important fishing areas in the Nile Delta. It is exposed to different quantities of serious pollutants in particular metals. To overall appraise the risk and status of metals in the lake, a comprehensive study of total concentrations of cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), and zinc (Zn) in sediments and spatial-temporal variations of these metals in the dissolved and suspended particulates, and some tissues of Tilapias niloticus, was conducted from ten sampling sites during 2016. Results showed that none of the investigated metals exceeded the limits considered as hazardous for aquatic life in water. The highest concentrations of Cd, Cu, Ni, and Zn were observed in suspended particulate matter, which may precipitate on the surface of the sediments. Potential ecological risk analysis of the majority of the investigated metals in the sediment indicated that Lake Edku posed a low ecological risk. The estimated values of all metals in tissues of Tilapia niloticus were below the international permissible limits. Moreover, the potential risk of metals to human via the consumption of Tilapia niloticus was estimated using the weekly intake levels, which was lower than the WHO's safe provisional tolerant weekly intake levels. These results prove the importance of performing measurements of contaminants in various compartments of Lake Ecosystem including sediment, biota, and suspended particulate matter for proper management.