Growth performance, carcass composition, and qualitative meat features of broiler chickens after galactooligosaccharides and sodium butyrate in ovo administration.

The study aimed to analyze the growth performance, feed indicators, and quantitative and qualitative physicochemical features of carcass and meat from broiler chickens after rearing, stimulated in ovo on d 12 of incubation with various substances. In the experiment, 1,200 hatching eggs from meat-type hen Ross 308 parental flock were incubated. On d 12, the injection was performed. Group CON-0 was noninjected. Group CON-S was injected with saline. In the GOS group, the procedure was performed in ovo with galactooligosaccharides (dissolved 3.5 mg of GOS in 0.2 mL of NaCl). In the SB group, butyric acid sodium salt was administered in a 0.3% SB dissolved in 0.2 mL NaCl. After hatching, 336, 1-day-old chicks per group were transferred to the broiler house and kept in 7 pens with 12 chickens per group for 42 d. The body weight and feed intake indicators were calculated. Next, 40 birds were selected (n = 10 per group) and taken to analyze carcass composition and meat quality (pH, color, WHC, drip loss, chemical composition). Compared to the experimental groups, the highest body weight indicators were found in groups CON-0 and CON-S. The feed conversion ratio was the lowest in the SB group on d 36 to 42 (P < 0.05). The European Efficiency Production Factor in groups GOS and SB was lower than in group CON-S (P = 0.005). The GOS group showed higher pH24hours in the pectoral muscles than the CON-S group (P = 0.011). The leg muscles showed better WHC in the CON-S, GOS, and SB groups than in the CON-0 group (P < 0.001). A lower intramuscular fat of the pectoral and leg muscle content was demonstrated, especially in the SB group. Injection of galactooligosaccharides and sodium butyrate in ovo adversely affected broiler production but did not alter carcass composition. It varied pectoral muscles' pH and chemical composition and improved water holding capacity and chemical composition in leg muscles.

Pheasant hatchability and physicochemical features of egg and extra-embryonic structures depending on eggshell color.

The study aimed to analyze the biological value of eggs and extra-embryonic structures affecting pheasant hatchability depending on the eggshell's color. Eggs (1,415) from 62-wk-old pheasants were used. The quality of fresh blue (BL), brown (BR), and green (G) eggs were analyzed. Incubation lasted for 25 d. Thick albumen (d 0, 1, 7, 14), amniotic fluid (d 14, 18), and the yolk (d 0-14) were collected. The pH, viscosity, lysozyme activity, crude protein (CP) content in albumen and amnion, pH, vitelline membrane strength, and fatty acids (FA) content in the yolk were performed. The lowest hatchability was in the BL group, and the highest was in the G group. BL group showed lower eggshell thickness and strength and higher egg weight. In thick albumen and amniotic fluid, the pH decreased with the incubation. In the yolk, there was an increasing trend (P = 0.015), with a decrease on d 18 (P < 0.001). The vitelline membrane strength decreased after 1 d of incubation, excluding BR eggs (P < 0.001). Thick albumen viscosity was higher on d 14 in the G group than in other dates and groups, the lowest in amniotic fluid, and slightly higher in BL and BR eggs. On d 18, amniotic fluid viscosity increased (P < 0.001). The lowest viscosity was indicated in BL eggs (P < 0.001). The lysozyme activity in thick albumen on d 14 was the highest (uniquely in BR and G groups), and the lowest values were found in amniotic fluid on d 14; after four d, the activity increased (P < 0.001). The CP content was higher in the BL group on d 14. In amnion, on d 14, the CP content was the lowest (<1%) and increased on d 18 (P < 0.001). There was a higher FA content (especially UFA) in the G group and a decrease in FA content after d 14 (P < 0.001). It was found that eggs with green eggshells have the highest biological value, and blue eggs are the least useful for incubation.

Changes in physicochemical parameters of duck eggs and extra-embryonic structures during incubation.

Duckling embryogenesis should be deepened due to the hatching technology and its modification possibilities. Many changes occur in incubated eggs, which expose the embryo to hazards. The study aimed to analyse the physicochemical properties of eggshell, yolk, thick albumen (TA), and amniotic fluid (AF) of incubated hatching eggs from 52-week-old Cherry Valley ducks. The morphological features of 18 fresh eggs were analysed. Over 28 days, a total of 800 eggs underwent incubation. Eggshell surface temperature and egg weight loss were measured on days 1, 4, 7, 10, 14, 18, 21, and 25. Eggshell, TA, AF, and yolk were collected from eggs at incubation days 1-21 (every week). TA was collected on days 0, 1, and 7, while AF on days 7, 14, and 21. The analysis covered a range of physicochemical parameters. Eggshell thickness decreased with incubation, reaching its lowest point posthatch (P < 0.001). The highest pH for TA was recorded on day 1, while the lowest was on day 7 when comparing days 0, 1, and 7 (P < 0.001). TA pH was consistently higher than in AF (P < 0.001). However, the pH of TA was the highest on day 1 and the lowest on day 7 (P < 0.001). Yolk pH increased from days 1 to 21 (P < 0.001). There was also a noticeable in egg weight loss (0.34% daily) (P < 0.001). Vitelline membrane strength decreased from day 0 to day 1 (P < 0.001). Lysozyme activity in thick albumen on day 7 was higher than on days 0 and 1 (P < 0.001). Lysozyme activity in AF was higher on day 21 than days 7 and 14 (P < 0.001). TA viscosity was highest on day 0 and lowest on day 1, compared to other days (P < 0.001). AF viscosity and CP content exhibited an increase on day 21 as compared to days 7 and 14 (P < 0.001). The CP content in TA was notably higher on day 7 than on days 0 and 1 (P < 0.001). Polyunsaturated fatty acids declined, while monounsaturated and transfatty acids increased (P < 0.001). Viscosity and lysozyme activity increased on day 7 in TA and day 21 in AF. TA and the amniotic cavity appeared to facilitate the transfer of substances, particularly CP. Viscosity could be an indicator for optimising incubation conditions, as incorrect changes can affect embryo mortality. The results showed the different utilisation of nutrients, such as fatty acids. It could support research on the in-ovo administration of various substances.

Use of coffee husks - comparison of pellet bedding quality, performance features, and some welfare indicators of broiler chickens.

BACKGROUND: The study aimed to evaluate the influence of wheat straw and different coffee husk (CHs) levels in pellet bedding on its quality, broiler chickens' performance, meat quality, and welfare indicators. In total, 200 Ross 308 chickens were divided into 4 groups: C - control with wheat straw pellet; CH10 - pellet with 10% CHs, CH25 - pellet with 25% CHs, and CH50 - pellet with 50% CHs. During 42 days of rearing, each bedding's physicochemical features were analyzed. The production results were controlled, and the footpad dermatitis, hock burns, and feather quality were assessed. From chosen birds, carcass composition was analyzed, as well as the qualitative features (color, water-holding capacity, drip loss) and breaking bone strength. RESULTS: The bedding material and rearing days influenced the content of dry matter, crude fiber, nitrogen, phosphorus, potassium, NDF, ADF, and pH. The results were inconclusive. The increasing trends in nitrogen, phosphorus, and potassium content were noticed at the end of rearing. Strong coefficient determination in bedding features was found (0.580 - 0.986). The pellet with CHs had no adverse effect on the growth performance of broilers. In the CH50 group, a lower fat percentage was found. A beneficial effect on water-holding capacity was noticed in leg muscles from CH10 and pectoral muscles from CH25. A significant decrease was found in footpad dermatitis incidence in groups CH25 and CH50. CONCLUSIONS: It can be concluded that CHs reuse in broilers as the pellet bedding material is possible due to the beneficial effect on some meat quality features and no adverse effect on the performance of broiler chickens. The positive impact on lower foot pad dermatitis incidence indicated the possibility of using CHs in pellet bedding.

The biological value of hatching eggs of broiler chicken in the early-mid incubation period based on physicochemical and morphologic features.

The study aimed to assess various quality characteristics (physical, morphologic, mechanical) of hatching eggs during the early-mid incubation period. Hatching eggs (1,200) were bought from a broiler Ross 308 breeder flock. Before incubation, 20 eggs were analyzed for dimensions and morphologic composition. Eggs (1,176) were incubated for 21 d. Hatchability was analyzed. On d 1, 2, 4, 6, 8, 10, and 12, eggs were collected (n = 20). The eggshell surface temperature and water loss were measured. The eggshell strength and thickness and the vitelline membrane strength were analyzed. The pH of thick albumen, amniotic fluid, and yolk were determined. The viscosity and lysozyme activity were studied for the thick albumen and amniotic fluid. Water loss was proportional and significantly different between incubation days. The yolk vitelline membrane strength highly depended on incubation days, decreasing steadily within the first 2 d (R2 = 0.9643). The albumen pH decreased from d 4 till d 12 of incubation, whereas the yolk pH first increased from d 0 to d 2 before a decline on d 4. Albumen viscosity was highest on d 6. There was a strong dependence of viscosity decrease with increasing shear rate (R2 = 0.7976). On the first day of incubation, the highest lysozyme hydrolytic activity was demonstrated (33,790 U/mL) compared to the activity from the amniotic fluid (8-12 d). From d 6, lysozyme activity decreased to 70 U/mL (d 10). On d 12, amniotic fluid lysozyme activity increased by over 6,000 U/mL compared to d 10. The lysozyme hydrolytic activity was lower in the amniotic fluid (d 8-12) compared to the thick albumen (0-6 d) (P < 0.001). The embryo's protective barriers are changed, and the fractions are hydrated during incubation. It could be concluded that the lysozyme is transferred from the albumen to the amniotic fluid due to its activity.

Production Efficiency and Utility Features of Broiler Ducks Fed with Feed Thinned with Wheat Grain.

The aim of this study was to evaluate the production efficiency (economics), growth, and meat quality of ducks fed with feed partially replaced with wheat. A total of 200 ducks were reared for 49 days. Each group consisted of 50 ducks (5 pens with 10 birds). For slaughter, 10 birds per group were chosen. The control group (C) was provided with a complete feed. In the experimental groups, from 42 to 49 days, the feed was replaced with wheat grains at the level of 10% (W10), 20% (W20), or 40% (W40). In the W20 and W40 groups, the cost of feed was reduced. In the W40 group, the profit per 1 kg carcass was increased by PLN 3.34 (more than 24% higher than the C group profit). A higher percentage of pectoral muscles and intramuscular fat was observed in the W20 group, with lower water content. A lower water-holding capacity (WHC) was observed in the duck leg muscles in group W40. The muscles from the W20 group had higher protein, collagen, and water content, and the fat was highest in the W40 group. A lower toughness of cooked meat was observed in the W20 group, and lower shear force in the pectoral muscles of groups C and W40. Thinning feed with wheat grains could represent an alternative to conventional feeding of broiler ducks, owing to reduced feed costs, with no negative impact on utility features, including growth, except the share of pectoral muscle and water absorption traits.

Growth of broiler chickens, and physical features of the digestive system, and leg bones after aluminosilicates used.

The assessment of aluminosilicates' impact on the production of chickens, the physical features of the intestines, and leg bones was done. 500 Ross 308 chickens were used and divided into 5 groups. The control group was I. Groups II, III, IV, and V were fed with halloysite and zeolite (1:3 ratio) at 0,5% (1-35 days; starter, grower 1 and 2) and 1% (36-42 days; finisher) levels. Aluminosilicates were also used for the peat litter: II-500 g of halloysite/m2; III-250 g of halloysite/m2 and 250 g of zeolite/m2; IV-500 g of zeolite/m2; V-130 g halloysite/m2, 370 g zeolite/m2. During 42 days, growth and feed indicators were recorded. 10 birds from each group were selected for slaughter. The digestive tract, femur, and tibia bones were sampled, and physical features were analyzed (weight, length, and strength). A lower feed conversion ratio on days 23-35 was found in the groups with the aluminosilicates addition. In group V a lower weight of the gizzard was found than in group I. A liver weight was higher in group V than in group III. A higher strength of the femurs was demonstrated in group IV. The tibia bones were characterized by higher strength than the femurs of broiler chickens. The aluminosilicates to feed and litter had no adverse effect.

Goose Embryonic Development, Glucose and Thyroid Hormone Concentrations, and Eggshell Features Depend on Female Age and Laying Period.

This study aimed to evaluate embryonic development; analyze the glucose, triiodothyronine (T3), and thyroxine (T4) concentrations in the blood of embryos and goslings; and assess the structure and temperature (EST) of the eggshell. The eggs that were analyzed were from four laying seasons of White Koluda® geese at three periods (90 eggs × 4 groups × 3 periods). The different embryo proportions, fetal membranes in the egg, and sizes of internal organs indicate a different growth rate and degree of embryo development depending on the laying age and laying period. The goose age influenced the hormone concentrations in the embryos' blood on the 28th day of incubation, which supports a relationship between the females' age and development. The eggshell thickness and density change depending on the laying age and the laying period. A decrease in eggshell thickness in the eggs up to the third season was found after the 16th day of incubation (simultaneously, the density showed an increasing trend). A lower EST distinguished the eggs from the oldest geese in the first half of the hatch. The formation of the chorioallantois membrane was associated with an increase in EST in the oldest geese.

The age of the geese from the parent flock and the laying period affect the features of the eggs.

The study aimed to assess the goose hatching egg features in four reproductive seasons from 3 stages of laying. Three hundred sixty eggs were used in the study from geese in the first, second, third, and fourth laying season. From each group, 90 eggs were analyzed (30 eggs from the beginning, the peak, and the end of the laying cycle). The structure of the egg and morphological and physical features of the yolk, albumen, and eggshell were analyzed. It was shown that the weight and structure of eggs increased, but the shape index was lower in 2-yr-old geese, as well Haugh's units decreased. The yolk share was lower in the first year, but albumen and eggshell were higher than in other groups. The eggshell whiteness was higher in the first year than in the second, and third. The pores' quantity was higher in the first year in the blunt and equatorial parts, but the total number in the egg was the highest in the fourth year. The yolk, albumen, and eggshell' density increased with the age. Changes in laying periods were inversely proportional to the changes shown depending on the layers' age. Geese's age and laying period impact the eggs' features. Based on the egg quality features, the incubation conditions could be adapted, as well as it can be treated as an indicator of the effectiveness of hatching and goslings quality. Research has shown that the biological value of hatching eggs changes with the age of the geese and the laying period.

Effect of parent flock age on hatching, growth rate, and features of both sexes goose carcasses.

The study aimed to assess the hatchability of goslings from parent flocks of geese in 4 laying seasons and to analyze the growth, carcass, and muscles characteristics. The hatching eggs from the White Koluda geese from the 1st to 4th laying season were incubated by the waterfowl hatching technology. Hatchability rates were calculated. 40 goslings were selected from each group. The geese were reared and fattened for 16 wk, (sex ratio of 1:1). From the 1-day-old goslings and at the end of the fattening period, the pectoral muscles were sampled to evaluate the muscle fibers. The body weight of the geese and the growth rate were analyzed. Body measurements were taken on the day of slaughter (6 birds/each group). The dissection was performed and the tissue composition of the carcasses was analyzed, including the percentage of carcass elements. The results were analyzed in terms of the age of the parent flock and the sex of oat geese. Hatchability performance was similar in all groups. Lower body weight of geese from group I was demonstrated at 0, 1, 7, 10 to 12 wk compared to birds from older geese. The growth rate in this group was higher than in groups III (2nd wk) and IV (1st wk). From 4 wkof age, the males had a higher body weight. In 1-day-old male goslings, a higher diameter of muscle fibers was demonstrated than in females. The body measurements of ganders were significantly higher compared to females, except for the length of the jump. Males were characterized by a higher weight of carcass parts. However, the share of abdominal fat was higher in females. No significant differences were found in the remaining features. Geese from different ages' parent flocks don't differ in the carcass features (the compensation phenomenon). The sex of the geese was influenced. It is reasonable to hatch goslings for fattening from parent flocks during 4 years of reproductive use.

Effect of Zeolite Supplementation on Gene Expression in the Intestinal Mucosa in the Context of Immunosafety Support in Poultry.

Zeolite is an effective and non-toxic silicate mineral. Its properties are widely used in industry due to its sorption and ion exchange properties. Due to its excellent chemical properties, it has also great potential in poultry production as a food additive or supplement to bedding. This is of great importance for the biosafety and hygiene of production. The study aimed to analyse the effects of simultaneous application of zeolite to feed and bedding on production parameters and expression of genes related to intestinal tightness, organism defence, and immune response. Male Ross 308 broiler chickens were used in the experiment. In the experimental group, an external factor in the form of a powdery zeolite was used for feed and pelleted bedding. On the day of slaughter, the caecal mucosa was collected for gene expression analysis. We showed no significant changes in the tissue composition of the carcasses, but zeolite had a beneficial effect on the carcass yield. The analysis of the immune gene panel showed a significant increase in the expression of the interleukins and interferons genes. We have demonstrated the effect of zeolite on the improvement of the intestinal barrier and increasing the tightness of the intestines. There were no changes in gene expression related to the host's defence against infections; therefore, based on the obtained results, it was concluded that zeolite can be considered an immunomodulating factor of the immune system.

The effect of partial replacement of milled finisher feed with wheat grains on the production efficiency and meat quality in broiler chickens.

The study's aim was to assess the production efficiency, evaluate the carcass and meat quality of chickens fed with wheat grains. 200 Ross 308 chickens were divided into 4 groups (5 replicates with 10 birds in each): control (C) and experimental groups, including W50, where the finisher feed was diluted with wheat grain in 50%, W25-25%, and W10-10%. The production efficiency and chemical composition of the feed were analyzed. After 42 d of rearing, 10 birds from each group were selected, and the tissue composition, pH, color, water-holding capacity, drip loss, the chemical composition of meat, and the apparent protein digestibility, bone, and jejunum strength were investigated. It was proved that ground feed had an unfavorable effect on the body weight (BW) in all groups. Wheat decreased the protein level (P < 0.001) and digestibility (P < 0.001). The body weight gain (BWG) in group W50 was lower than in groups C and W10 (P = 0.009), however, this had no effect on the final feed conversion ratio (FCR) (P = 0.146). Finisher feed costs were reduced in groups W50, W25 compared to group C (P < 0.001). The European Production Efficiency Factor and the European Broiler Index in groups W10 and W25 were similar to group C, whereas in W50 they were reduced (P = 0.035; 0.034). No negative effect on carcass traits was shown in groups W10 and W25, however, 50% feed replacement was unbeneficial compared to group C. Pectoral muscles from the experimental groups were characterized by higher lightness (P < 0.001). In group W10 femur bones' strength and in group W25 tibia bones' strength was higher than in group W50 (P = 0.014; 0.006). Jejunum tensile strength was higher in group W25 than in W10 (P = 0.002). The nutritional strategies based on the dilution of the feed with wheat grain could be applied at the level of 10/25%, but 50% had a negative effect.

Research Note: Growth and meat features of broiler chicken with the use of halloysite as a technological additive to feed and peat litter.

The current trends in the production of broiler chickens indicate the need to look for natural solutions that affect the efficiency of production and the quality of meat. The aim of the study was to evaluate the growth performance and quality of broiler chicken meat with the addition of halloysite in feed and litter. Two hundred Ross 308 were used and divided into 2 groups (10 replicates each). The control group (C) did not have any additive, while in the experimental group (H) 0.5% halloysite was used in feed and 0.500 kg/m2 in peat litter. The production results and litter traits were checked. The presence of footpad dermatitis (FPD) was assessed. After 42 d, 20 birds were randomly selected and slaughtered. Dissection was performed. The slaughter yield and the percentage of carcass elements were calculated, including offal. Breast and leg muscles were analysed qualitatively (pH, colour, water-holding capacity, drip loss, chemical composition). In group H there were no changes to FPD, and in group C there were 6 cases of benign lesions and 1 with severe damage and scabs on the soles of the feet. Significantly higher preslaughter body weight was shown, as well as carcass and wings weight in group H. The H group had a higher pH24 than the C group, as well as higher protein and water content, and lower intramuscular fat and salt, both in the breast and leg muscles. No significant differences were found in growth performance carcass, meat' colour or water-holding capacity features. The litter with halloysite was characterized by a lower pH but without statistical confirmation. Addition of halloysite to feed and litter reduced the occurrence of skin lesions and had a positive effect on higher protein content and lower intramuscular fat in the breast and leg muscles. This demonstrates the potential for the practical use of halloysite in the production of broiler chickens, both as a feed and peat litter additive.

Aluminosilicates at different levels in rye litter and feed affect the growth and meat quality of broiler chickens.

Litter sanitation treatments and feed supplements that stimulate bird growth. The aim of this study was to analyse the effects of zeolite (z) and halloysite (h) in feed and rye straw litter on growth performance, meat quality in chickens. 500 males Ross 308 were allocated to 5 groups (10 replicates). Feed for groups 2-5 was supplemented with halloysite and zeolite (25:75). The content of aluminosilicates in the feed was different depending on the feeding phase: 0.5, 1, 1.5, or 2%. The following doses were used in litter: 2, 0.800 kg/m2 h; 3, 0.400 kg/m2 h and 0.400 kg/m2 z; 4, 0.800 kg/m2 z; 5, 0.200 kg/m2 h, and 0.600 kg/m2 z. Growth, and meat quality were analysed. Body weight and body weight gain were higher in groups 2-5 than in group 1, while feed intake was lower in groups 1 and 2 (p < 0.05). Body weight, the weight of carcass, and most of its elements were higher in the experimental groups (p < 0.05). Breast muscles from group 1 were characterised by better water-holding capacity and higher protein content, while those from 4 had higher content of collagen and water (p < 0.05). Breast muscles from group 3 were characterized by lower yellowness than in 2 (p < 0.05). Leg muscles from group 1 were characterized by lower redness than in 4 (p < 0.05). Water-holding capacity was better in group 3 and protein content was higher in 2 (p < 0.05). The content of fat in leg muscle was lower in all experimental groups compared to control (p < 0.05). This indicates the suitability of aluminosilicates in poultry management practice, with better growth and meat quality.

Growth performance and meat quality from broiler chickens reared with zeolite and halloysite in feed and straw pellet.

The study's aim was to analyze the effects of different levels of halloysite (H) and zeolite (Z) in feed and pellets on the growth and meat quality in chickens; 500 Ross 308 broilers were assigned to five groups (1 g, control; 2-5 g, experimental). In Groups 2-5, different proportions of Z and H in feed (25:75 ratio; 0.5% to 2%) and pellet in kg/m2 (2, 0.650H; 3, 0.325H and 0.325Z; 4, 0.650Z; 5, 0.160H and 0.490Z) were used. Body weight was higher than 1 in all experimental groups, and carcass weight was higher, except for Group 2. The feed consumption was lower in Groups 3 and 5 than in Group 4. The breast muscle weight was higher in Group 3 than in the control group. In Groups 2, 3, and 5, the water holding capacity in the breast muscles was better than in Group 4 and in the leg muscles in Groups 3 and 4 than in Groups 1 and 5. Most of the tested characteristics indicate a beneficial effect of aluminosilicates in feed and litter on the growth and quality of meat.

Wheat litter and feed with aluminosilicates for improved growth and meat quality in broiler chickens.

BACKGROUND: Natural minerals have many beneficial properties in poultry production, taking into account production as well as hygiene, health, safety, and quality of broiler meat. The aim of the study was to evaluate the effect of aluminosilicates in feed and litter on the growth performance and meat quality in chickens. Aluminosilicates, including halloysite and zeolite, could be a good alternative for synthetics, as a good solution for the environment in line with the current trends. METHODS: Five-hundred male Ross 308 chickens were managed in 5 groups (10 replicates/group): LITTER: 1, control; 2, 0.95 kg/m2 of halloysite; 3, 0.475 kg/m2 of halloysite and 0.475 zeolite; 4, 0.95 kg/m2 of zeolite; 5, 0.25 kg/m2of halloysite and 0.7 kg/m2 of zeolite. FEED: groups 2-5, halloysite and zeolite addition (25:75 ratio; 0.5-2%). Growth performance (body weight and feed indicators), carcass, and meat quality (pH, colour, water-holding capacity, chemical composition of muscles) were recorded. The experimental setup, where the aluminosilicate additives were applied simultaneously, was proposed and approved by experts after pilot testing and on the basis of extensive literature where feed or litter additives were tested. RESULTS: Body weight and its gain were higher in groups 3 and 4 than in 1, and feed intake was higher in 4. The weight of the carcass and some of its components, including muscles and skin with subcutaneous fat, were higher in 2-4. Water loss from leg muscles was lower in 4. The content of protein in muscles was significantly higher in 3. The addition of aluminosilicates in feed and litter had a positive effect on the growth performance and some traits of carcasses and meat quality, especially in group 3. Halloysite and zeolite can be used in feed and litter (especially 0.475 kg/m2 for each mineral in the wheat litter).

The Impact of Hydrated Aluminosilicates Supplemented in Litter and Feed on Chicken Growth, Muscle Traits and Gene Expression in the Intestinal Mucosa.

The aim of the study was to compare the production, muscle traits and gene expression in the intestinal mucosa of chickens supplemented with aluminosilicates in feed and litter simultaneously. A total of 300 Ross 308 were maintained for 42 days. Group 1 was the control group. In group 2, 0.650 kg/m2 of halloysite was added to the litter and 0.5-2% to the feed (halloysite and zeolite in a 1:1 ratio); in group 3, we added zeolite (0.650 kg/m2) to the litter and 0.5-2% to the feed. The production parameters, the slaughter yield and analyses of muscle quality were analyzed. There was a higher body weight, body weight gain and feed conversion ratio on day 18 and 33 in group 3, and a higher feed intake on day 19-33 in groups 2 and 3 than in 1. A lower water-holding capacity was found in the breasts of group 2 and in the legs of group 3 compared to group 1. The expression of genes related to the immune response, host defense and intestinal barrier and nutrient sensing in the intestinal tissue was analyzed. The results show a beneficial effect on the immune status of the host without an adverse effect on the expression of genes related to intestinal tightness or nutritional processes. Due to the growth, meat characteristics and the positive impact of immunostimulant and regulating properties, aluminosilicates can be suggested as a litter and feed additive in the rearing of chickens.

Characteristics of carcass and physicochemical traits of meat from male and female ducks fed a diet based on extruded soybean.

Duck' meat is characterized by good nutritional properties and gaining popularity in the consumer market. Extruded soybean is potentially more digestible than commonly use soybean meal (SBM), and is expected to influence carcass traits and the quality of breast and leg muscles. The study' aim was to compare meat quality from both sexes' ducks fed a diet with extruded soybean (ESB) as a substitute for SBM. Cherry Valley ducks were divided into two groups. The control group (1) was fed an SBM-based diet, and the treatment group (2) with ESB. Each group was divided into sex subgroups with 50 birds in each (5 replicates, 10 ducks each). Dissection and analysis of meats' pH, colour, water-holding capacity (WHC), drip loss and chemical composition of breast and leg muscles were done. Interaction of Diet and Sex was calculated. In group 2 higher carcass weight, dressing percentage, weight of wings, leg muscles, total muscles, and better WHC were found. Dressing percentage, the proportion of neck with skin, breasts' and stomach' weight, and the weight and proportion of fat, and pH45min were higher in females (P < 0.05). The interaction was found for the pre-slaughter body weight, the weight of carcass remains, WHC in breasts (P < 0.05). The ESB feed had no negative effect on the analyzed traits and can be used in the ducks' diet. Improved the WHC indicates the high suitability of meat for processing. A positive effect of diet on the muscles' proportion and dressing percentage was noticed, which is important for consumers' market. The sex-related differences and interactions between variables suggest separate rearing due to sex.

Ducks' Growth, Meat Quality, Bone Strength, and Jejunum Strength Depend on Zeolite in Feed and Long-Term Factors.

The safety of production and the high quality of meat are important aspects of rearing poultry, especially when natural solutions are used. Because of the increasing popularity of duck meat, the aim of the present study was to assess the effect of a diet with an inclusion of zeolite on the growth performance, meat quality, and strength of the bones and jejunum in ducks of different origin, sex, and age at slaughter. The study was conducted on 320 Orvia and Cherry Valley ducks. Birds were allocated to eight groups, according to their sex and origin. Half of the birds received feed with a 4% inclusion of zeolite. Body weight gain, feed intake, and feed conversion ratio per kilogram of gain were calculated. After six or seven weeks, five birds from each group were selected and slaughtered. After dissection, meat quality (pH, water-holding capacity, colour) and the strength of the bones and gut were analysed. The analysis revealed that zeolite was associated with reduced body weight gains and increased feed conversion ratio, but with a higher water-holding capacity of breast muscles in seven-week-old ducks, and with a higher yellowness and water-holding capacity of leg muscles in six-week-old ducks. A positive effect of long-term factors (age, sex, origin) and the interaction with zeolite was found for most of the analysed traits. Findings on the effect of 4% inclusion of zeolite in duck diet were inconclusive. The study implies the need for further research, since zeolite has potential as a natural sanitizing agent and can improve the quality of produced duck meat.

Egg quality depending on the diet with different sources of protein and age of the hens.

Due to the planned limitations in the use of genetically modified soybean meal, the search for alternative sources of protein in animal nutrition is ongoing, which also supports the consumers' expectations of good quality products, such as eggs. The aim of the study was to assess and compare morphological traits of eggs, fatty acid composition in yolk lipids, and the content and activity of lysozyme in thick and thin albumen in eggs from hens fed a diet based on legume seeds as a substitute for soybean meal depending on the hens age. Analyses were carried out for 300 eggs on dates I-V (19-39 weeks age of hens), obtained from Rosa 1 hens managed in the semi-intensive system. Quality analysis was performed for 30 eggs from each group at a time. The control group of hens (A) was fed a diet based on soybean meal (SBM) and the treatment group (B) was fed a diet based on seeds from narrow-leaved lupin (Boruta), yellow lupin (Mister), and pea (Muza). Eggs were analysed for morphological traits (egg weight, the weight and density of egg components, egg shape index and egg surface area), parameters of albumen (height, Haugh units), yolk colour (La Roche, colorimetry in the CIE Lab system), lysozyme content and activity in albumen, and fatty acids composition in yolk lipids. In group B the egg shape index was higher (p = 0.002), and the eggshell colour index was lower (p = 0.007), as well as the height of thick albumen, Haugh score, and yolk colour (La Roche) were higher in group B (p < 0.05), while redness of yolk was significantly lower (p < 0.001) than in group A. Lower content of C15:0 and C18:1n9 and higher content of C18:2n6 and C18:3n3, (p < 0.05), as well as lower content of MUFA and OMEGA 9, but higher content of PUFA, OMEGA 3, OMEGA 6, PUFA/SFA, OMEGA 9/6 and OMEGA 9/3 were found in group B compared to group A (p < 0.05). There were significant differences in traits between age of hens (p < 0.05). Egg weight, yolk weight and its proportion in egg, as well as shell weight, its strength and thickness increased with the age of hens (p < 0.05). The content of lysozyme and its activity were also higher in eggs from older hens (p < 0.05). Fatty acids composition was beneficial at the beginning and end of the analysed egg production period. The proposed feed mixture based on legume seeds had no negative effect on the quality of eggs, and had a positive effect on yolk colour, the quality of albumen. There was no detrimental effect of diet and age of hens on fatty acid composition of eggs from both groups throughout the study period. To sum up, diet with legume seeds could be accepted as a substitute for soybean meal, due to the beneficial effects for some mentioned traits of eggs. Use of legumes could give a partial self-sufficient for producers from small farms, where is not possible to produce soybean meal. At the same time, the level of anti-nutrients in legumes should be considered.