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1.
Bull Exp Biol Med ; 169(5): 639-643, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32979126

RESUMEN

The presence of a complex of lipoprotein(a) and proprotein convertase subtilisin/kexin type 9 (PCSK9) in the blood of healthy volunteers and patients with cardiovascular diseases was analyzed by ELISA. The levels of the complex varied in a wide range and did not depend on the concentrations of Lp(a) and PCSK9. Moreover, the complex was found not only in patients with cardiovascular diseases, but also in healthy volunteers, which can indicate physiological role of lipoprotein(a) as PCSK9 transporter.


Asunto(s)
Lipoproteína(a)/metabolismo , Proproteína Convertasa 9/metabolismo , Enfermedades Cardiovasculares/genética , Enfermedades Cardiovasculares/metabolismo , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Lipoproteína(a)/genética , Masculino , Proproteína Convertasa 9/genética
2.
Bull Exp Biol Med ; 167(1): 24-29, 2019 May.
Artículo en Inglés | MEDLINE | ID: mdl-31177468

RESUMEN

Increased concentration of lipoprotein(a) is a risk factor of coronary heart disease. lipoprotein(a) consists of LDL-like and highly polymorphic apolipoprotein(a). Here we studied the effect of lipoprotein(a)-containing sera with different apolipoprotein(a) phenotypes on lipid accumulation by THP-1 monocyte-like cells. Cholesterol concentration in lysates of THP-1 cells was significantly higher after their incubation with lipoprotein(a)-containing serum samples with low-molecular-weight phenotype of apolipoprotein(a) in comparison with samples with a high-molecular-weight apolipoprotein(a) phenotype irrespective of initial cholesterol level as well as serum concentrations of apoB-100, oxidized LDL, and circulating immune complexes. The presence of the most atherogenic small dense LDL subfractions in examined sera in addition to a low-molecular-weight apolipoprotein(a) phenotype resulted in significant elevation of cholesterol accumulation by THP-1 cells. The data obtained explain greater atherogenicity of lipoprotein(a) with low-molecular-weight apolipoprotein(a) phenotype.


Asunto(s)
Apolipoproteínas A/metabolismo , Colesterol/metabolismo , Apolipoproteínas/metabolismo , Medio de Cultivo Libre de Suero , Humanos , Macrófagos/metabolismo , Monocitos/metabolismo , Células THP-1
3.
Bull Exp Biol Med ; 166(3): 330-333, 2019 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-30627915

RESUMEN

In a 2-year prospective study, prognostic significance of the blood content of IL-10-producing CD4+ T lymphocytes for progression of coronary artery atherosclerosis was assessed. Patients with verified stable angina (n=36) admitted for scheduled coronary angiography and coronary stenting were enrolled. The blood levels of CD4+FoxpP3+ Treg, CD4+IFNγ+ Th1, CD4+IL17+ Th17, CD4+IL10+ cells, sCD25, IL-10, IL-17, C-reactive protein, and lipoprotein (a) were assayed before endovascular interventions. The blood content of CD4+IL10+ T cells below 3.3% was associated with progression of coronary artery atherosclerosis (OR 12.0 (2.3, 61.0), sensitivity 77%, specificity 78%, p=0.003). No differences in other immunological parameters and common atherosclerosis risk factors in the groups were revealed. We hypothesize that the content of CD4+IL10+ T cells can be an important predictive marker for the progression of coronary atherosclerosis.


Asunto(s)
Angina Estable/sangre , Aterosclerosis/sangre , Enfermedad de la Arteria Coronaria/sangre , Interleucina-10/sangre , Linfocitos T Reguladores/inmunología , Anciano , Angina Estable/diagnóstico por imagen , Angina Estable/inmunología , Angina Estable/patología , Aterosclerosis/diagnóstico por imagen , Aterosclerosis/inmunología , Aterosclerosis/patología , Biomarcadores/sangre , Proteína C-Reactiva/inmunología , Proteína C-Reactiva/metabolismo , Recuento de Linfocito CD4 , Angiografía Coronaria , Enfermedad de la Arteria Coronaria/diagnóstico por imagen , Enfermedad de la Arteria Coronaria/inmunología , Enfermedad de la Arteria Coronaria/patología , Progresión de la Enfermedad , Femenino , Humanos , Interleucina-10/inmunología , Interleucina-17/sangre , Interleucina-17/inmunología , Lipoproteína(a)/sangre , Lipoproteína(a)/inmunología , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Factores de Riesgo , Linfocitos T Reguladores/patología , Células TH1/inmunología , Células TH1/patología , Células Th17/inmunología , Células Th17/patología
4.
Bull Exp Biol Med ; 162(2): 231-236, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27905032

RESUMEN

We studied the composition of circulating immune complexes precipitated in the presence of various concentrations of polyethylene glycol in patients with coronary heart disease (CHD) and high concentration of lipoprotein(a) - Lp(a). Precipitation of highly purified Lp(a) preparation with polyethylene glycol was evaluated. The contents of Lp(a), autoantibodies to Lp(a), IgG, and IgM in circulating immune complexes isolated from the sera of donors and CHD patients with normal and high levels of Lp(a) were measured. Circulating immune complexes containing Lp(a) were detected in the plasma of CHD patients with high Lp(a) concentrations. The presence of high concentrations of Lp(a), autoantibodies to Lp(a), and circulating immune complexes in CHD patients suggests that immunological factor contributes to high atherothrombogenicity of Lp(a).


Asunto(s)
Complejo Antígeno-Anticuerpo/sangre , Aterosclerosis/diagnóstico , Autoanticuerpos/sangre , Enfermedad de la Arteria Coronaria/diagnóstico , Lipoproteína(a)/sangre , Apolipoproteína B-100/sangre , Aterosclerosis/sangre , Aterosclerosis/inmunología , Aterosclerosis/patología , Biomarcadores/sangre , Estudios de Casos y Controles , Precipitación Química , HDL-Colesterol/sangre , LDL-Colesterol/sangre , VLDL-Colesterol/sangre , Enfermedad de la Arteria Coronaria/sangre , Enfermedad de la Arteria Coronaria/inmunología , Enfermedad de la Arteria Coronaria/patología , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Polietilenglicoles/química , Triglicéridos/sangre
5.
Vopr Virusol ; 61(6): 263-269, 2016 Dec 28.
Artículo en Inglés | MEDLINE | ID: mdl-36494985

RESUMEN

The article presents the results of clinical studies on the comparative effectiveness of different forms of the drug Cytovir-3 (syrup and powder for solution for oral administration) and Immunal l in the treatment of acute respiratory viral infections in children. It was found in a comparative randomized parallel-group study of 90 children aged two through six years that the drug Cytovir-3 (syrup and powder) became active faster than the comparison drug Immunal, providing normalization of body temperature, reduction of some manifestations of the general intoxication and respiratory syndrome, as well as elevated levels of serum immunoglobulin A. All drugs in the study had an equally normalizing effect on the content of peripheral blood leukocytes and erythrocyte sedimentation rates. The compared products were characterized by good tolerability, lack of side effects and high preventive efficacy against respiratory disease complications. Cytovir-3 drugs (syrup and powder for oral solution) and Immunal had similar ratios of clinical and laboratory safety, efficacy and tolerability. Both products can be used for the treatment of respiratory diseases in children aged two through six years.

6.
Kardiologiia ; 54(6): 4-8, 2014.
Artículo en Ruso | MEDLINE | ID: mdl-25178070

RESUMEN

OBJECTIVE: Lipoprotein(a) - Lp(a) is an independent risk factor of atherosclerosis and its complications. In spite of the long period of Lp(a) research there is no complete understanding of its physiological role and atherogenic action. The goal of this study was to investigate the presence in human plasma of circulating autoantibodies to Lp(a) belonging to different classes of immunoglobulins, and to elucidate their relationship to the presence and severity of coronary atherosclerosis in middle aged patients with coronary heart disease. MATERIAL AND METHODS: Autoantibodies to Lp(a) and LDL level were measured in 97 men with quantitative angiography data. Among these patients 17 had intact vessels and 80 had stenoses >50% in 1 or more large coronary arteries. RESULTS: Lp(a) level in patients with coronary atherosclerosis (CA) was significantly higher (median 19.0 [95%CI 12.9-27.3] mg/dl) than in patients without CA (median 10.2 [95%CI 4.5-18.3] mg/dl, p=0.05). Level of IgG autoantibodies to Lp(a) was also elevated in patients with verified CA (264 ± 188 and 187 ± 59 RLU, respectively, p=0,003). Correlation analysis showed significant association between Lp(a) concentration and presence of CA (r=0.20, p=0.05). Level of antibodies against Lp(a) correlated with the quantity of affected coronary arteries (r=0.27, p=0.008). CONCLUSION: Plasma concentration of Lp(a) as well as elevated level of autoantibodies to Lp(a) were associated with the presence and severity of coronary atherosclerosis in examined patients.


Asunto(s)
Autoanticuerpos/sangre , Enfermedad de la Arteria Coronaria/inmunología , Enfermedad Coronaria , Lipoproteína(a)/inmunología , Anciano , Angiografía Coronaria/métodos , Enfermedad de la Arteria Coronaria/complicaciones , Enfermedad de la Arteria Coronaria/diagnóstico , Enfermedad Coronaria/etiología , Enfermedad Coronaria/inmunología , Enfermedad Coronaria/fisiopatología , Vasos Coronarios/patología , Humanos , Masculino , Persona de Mediana Edad , Factores de Riesgo , Índice de Severidad de la Enfermedad , Estadística como Asunto
7.
Kardiologiia ; 54(4): 10-5, 2014.
Artículo en Ruso | MEDLINE | ID: mdl-25177780

RESUMEN

We studied effect of cascade plasma filtration on subfractions of low density lipoproteins (LDL) in 16 patients with ischemic heart disease (IHD) and hyperlipidemias refractory to drug therapy. The procedure caused 37.6 ± 1.4%, 43.1 ± 2.5%, and 50.9 ± 1.4% lowering of total, low density lipoprotein cholesterol, and apolipoprotein B-100, respectively. The use of filters led to removal of larger less atherogenic LDL-1 and LDL-2 subfractions with relative accumulation of small dense LDL-3, LDL-4, and LDL-5 subfractions. Lowering of concentrations of all LDL subfractions was caused by specific LDL apheresis by immunosorbtion in 4 patients with IHD. Removal of small dense LDL was more effective than that of large less atherogenic LDL-1 and LDL-2 subfraction. Thus we found differences in spectrum of LDL removed by cascade plasma filtration and immunosorbtion. The use of filters was associated with less effective removal of most atherogenic small dense LDL than of large LDL particles what led to redistribution of concentrations of various LDL subfractions towards atherogenic profile of lipoproteins. These results could constitute a basis for revision of methods of conduct of cascade plasma filtration procedures in patients with IHD.


Asunto(s)
Hemofiltración/métodos , Hiperlipidemias , Lipoproteínas LDL/metabolismo , Isquemia Miocárdica , Adulto , Apolipoproteína B-100/sangre , LDL-Colesterol/sangre , Resistencia a Medicamentos , Femenino , Humanos , Hiperlipidemias/sangre , Hiperlipidemias/complicaciones , Hiperlipidemias/terapia , Hipolipemiantes/uso terapéutico , Lipoproteínas LDL/química , Masculino , Persona de Mediana Edad , Monitoreo Fisiológico , Isquemia Miocárdica/sangre , Isquemia Miocárdica/complicaciones , Isquemia Miocárdica/terapia , Resultado del Tratamiento
8.
Bioorg Khim ; 40(2): 166-9, 2014.
Artículo en Ruso | MEDLINE | ID: mdl-25895335

RESUMEN

DNA aptamer based sorbents are synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were from 11 x 10(-l0) to 1.7 x 10(-10) M depending on the orientation of the aptamer, an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were picked up. These chromatographic materials can be used for medical and biotechnological applications.


Asunto(s)
Aptámeros de Nucleótidos/química , Cromatografía , Inmunoglobulina E/química , Oligonucleótidos/química , ADN/química , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/aislamiento & purificación , Unión Proteica
9.
Klin Lab Diagn ; (4): 24-7, 2013 Apr.
Artículo en Ruso | MEDLINE | ID: mdl-23984550

RESUMEN

The article deals with specification of technique of immune-enzyme analysis to detect autoantibodies to beta-adrenergic receptors (beta1-AP) using compound of oligopeptids representing the fragmentations of extracellular sites beta1-AP and chimeric molecule of extracellular section of receptor This technique significantly exceeds the analogues defined in publications by its sensitivity and correlation with diagnosis.


Asunto(s)
Autoanticuerpos/sangre , Cardiomiopatía Dilatada/sangre , Receptores Adrenérgicos beta 1/aislamiento & purificación , Autoanticuerpos/inmunología , Cardiomiopatía Dilatada/inmunología , Ensayo de Inmunoadsorción Enzimática , Humanos , Péptidos/síntesis química , Péptidos/inmunología , Receptores Adrenérgicos beta 1/sangre , Receptores Adrenérgicos beta 1/inmunología
10.
Vopr Onkol ; 59(1): 72-7, 2013.
Artículo en Ruso | MEDLINE | ID: mdl-23814829

RESUMEN

There was investigated the relationship of lipid metabolism, humoral immunity, high sensitivity C-reactive protein and soluble receptors for TNF-alpha with the presence, severity and prognosis of the disease in 85 patients with morphologically verified diagnosis of prostate cancer. Connection with the dynamics of change in PSA as a major predictor of the disease was observed for concentration sRI and sRII to TNF, PSA baseline, total cholesterol and LDL-cholesterol. During follow-up the relative risk of poor prognosis increased by 3 times, and death-in 8.7 times with increasing concentrations of soluble receptors for TNF. The concentration of soluble receptors for TNF may serve as an additional predictor of the presence and clinical course of disease in patients with prostate cancer.


Asunto(s)
Biomarcadores de Tumor/sangre , Inmunidad Humoral , Metabolismo de los Lípidos , Neoplasias de la Próstata/metabolismo , Receptores Tipo II del Factor de Necrosis Tumoral/metabolismo , Receptores Tipo I de Factores de Necrosis Tumoral/metabolismo , Anciano , Anciano de 80 o más Años , Proteína C-Reactiva/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Valor Predictivo de las Pruebas , Pronóstico , Antígeno Prostático Específico/sangre , Neoplasias de la Próstata/inmunología , Neoplasias de la Próstata/mortalidad , Neoplasias de la Próstata/patología , Factores de Riesgo , Índice de Severidad de la Enfermedad
11.
Bioorg Khim ; 38(1): 58-63, 2012.
Artículo en Ruso | MEDLINE | ID: mdl-22792706

RESUMEN

New chromatographic material based on tryptophil-threonil-tirosine was prepared. This sorbent effectively binds human, sheep, goat and cow immunoglobulins G. New sorbent shows high selectivity for removing immunoglobulins from blood plasma. Effective sorption capacity is 15-25 mg of immunoglobulin G per ml of matrix. Optimal method of covalent attachment ligand to polysaccharide matrix allows achieving high stability of the sorbents in terms of use and storage. This sorbent can be used in medicine and biotechnology.


Asunto(s)
Cromatografía Liquida/métodos , Inmunoglobulina G/aislamiento & purificación , Oligopéptidos/química , Plasma/química , Humanos , Inmunoglobulina G/química
12.
Kardiologiia ; 51(5): 9-16, 2011.
Artículo en Ruso | MEDLINE | ID: mdl-21649590

RESUMEN

PURPOSE: To assess effects of niacin on risk factors of atherosclerosis in men with coronary heart disease (CHD) and high lipoprotein(a) [Lp(a)] levels. MATERIAL AND METHODS: Sixty men (mean age 54+/-6 years) with angiographic evidence of CHD were randomized into two groups. Active group (n=30) received extended release nicotinic acid 1500 mg, control group consisted of remaining 30 patients. All patients received basic therapy with atorvastatin 10-40 mg qd. Blood samples were collected for total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), Lp(a), lipoprotein-associated phospholipase A2 (Lp-PL-2), high-sensitivity C-reactive protein (hsCRP), complex of tissue-type plasminogen activator with plasminogen activator inhibitor type 1 (tPA/PAI-1). Carotid intima media thickness (CIMT) was measured at baseline and after 6-months therapy. RESULTS: There was no statistically significant difference between the groups in the clinical and biochemical characteristics. During the study lipid profile data were within the target levels. In the active group median percent decrease of Lp(a) level was 23% (from 84+/-40 to 67+/-25 mg/dl after 6 weeks and up to 65+/-37 mg/dl after 6 months of treatment, p<0.01); LDL-C, TG, tPA/PAI-1, and Lp-PL-2 mass levels decreased by 25, 20, 25, and 32%, respectively; HDL-C increased by 16% (p<0.05 vs baseline, respectively). Nicotinic acid treatment produced statistically significant reduction nicotinic acid of the mean CIMT (right: 0.83+/-0.16 vs 0.77+/-0.17 mm, p<0.05; left: 0.88+/-0.21 vs 0.82+/-0.17, p<0.05). In control group no changes of CIMT or blood tests were observed. CONCLUSION: In men with CHD and Lp(a) excess of addition to atorvastatin results in regression of CIMT on an average of 0.06 mm in 6 months. Such rapid and significant effect on the arterial wall structure can be attributed to the complex influence of nicotinic acid on Lp(a), lipids, Lp-PL-2 and thrombogenic factors. This is the first study providing the evidence of using Lp(a) as one of therapeutic targets in patients with high Lp(a) levels for achieving beneficial effect on a surrogate marker of atherosclerosis.


Asunto(s)
Aterosclerosis , Arterias Carótidas , Metabolismo de los Lípidos/efectos de los fármacos , Lipoproteína(a)/metabolismo , Niacina , Túnica Íntima , 1-Alquil-2-acetilglicerofosfocolina Esterasa/sangre , Aterosclerosis/complicaciones , Aterosclerosis/tratamiento farmacológico , Aterosclerosis/metabolismo , Aterosclerosis/patología , Atorvastatina , Arterias Carótidas/efectos de los fármacos , Arterias Carótidas/patología , Enfermedad de la Arteria Coronaria/tratamiento farmacológico , Enfermedad de la Arteria Coronaria/etiología , Enfermedad de la Arteria Coronaria/metabolismo , Quimioterapia Combinada , Ácidos Heptanoicos/administración & dosificación , Ácidos Heptanoicos/efectos adversos , Humanos , Hiperlipoproteinemias/tratamiento farmacológico , Hiperlipoproteinemias/etiología , Hiperlipoproteinemias/metabolismo , Hipolipemiantes/administración & dosificación , Hipolipemiantes/efectos adversos , Masculino , Persona de Mediana Edad , Niacina/administración & dosificación , Niacina/efectos adversos , Inhibidor 1 de Activador Plasminogénico/metabolismo , Pirroles/administración & dosificación , Pirroles/efectos adversos , Resultado del Tratamiento , Túnica Íntima/efectos de los fármacos , Túnica Íntima/patología
13.
Kardiologiia ; 51(1): 18-22, 2011.
Artículo en Ruso | MEDLINE | ID: mdl-21626797

RESUMEN

Study aim was to investigate the association of lipoprotein (a) [Lp(a)] level with the development of cardiovascular complications in long-term follow-up period after coronary artery bypass grafting (CABG). Patients with chronic ischemic heart disease (IHD) (n = 361, 88% men, mean age 55 +/- 9 years) who had had CABG were included in the study. Before surgery we assessed presence of classical risk factors, left ventricular ejection fraction, concentrations of lipids and Lp(a) in blood serum. During follow-up (from 1 to 140, mean 66 +/- 34 months) we registered cardiac deaths, nonfatal myocardial infarctions (MI), strokes, repeat procedures of revascularization, and hospitalizations due to relapse or progression of angina pectoris. Information on prognosis was obtained from 263 patients. In 109 of them we registered 142 serious events including cardiac death n = 20 (14%), nonfatal MI n = 14 (10%), myocardial revascularization (n = 35), 29 (20%) with stenting), repeat CABG n = 6 (4%), hospitalization due to angina pectoris n = 53 (37%), stroke n = 4 (3%), noncardiac outcome n = 16 (10%). In subjects with hyperlipidemia (a) [HLp(a) - Lp(a) > 30 mg/l] survival after CABG was lower (log rank p < 0.001): 11 of 93 (11.3%) and 9 of 170 (5.2%) patients died among those with Lp(a) > 30 and < 30 mg/I, respectively. Relative risk (RR) of any cardiovascular complication was 3.24 (95% confidence interval [CI] 2.18 to 4.83, p < 0.001), of death - 2.89 (95% CI 1.31 to 6.35, p < 0.01), and of MI A 1.01 (95% CI 1.00 to 1.02; p = 0.02). RR of development of MI and cardiac death in patients with HLp(a) in 5 years was 2.61 (95% CI 1.11 to 5.74; p = 0.02), in 10 years - 2.95 (95% CI 1.50 to 5.79; p < 0.001). In patients with chronic IHD high level of Lp(a) can serve as independent predictor of unfavorable events including death and nonfatal MI during 10 years after CABG.


Asunto(s)
Enfermedades Cardiovasculares , Puente de Arteria Coronaria/efectos adversos , Lipoproteína(a)/sangre , Isquemia Miocárdica , Complicaciones Posoperatorias , Enfermedades Cardiovasculares/epidemiología , Enfermedades Cardiovasculares/etiología , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/fisiopatología , Muerte , Femenino , Estudios de Seguimiento , Hospitalización , Humanos , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/diagnóstico , Isquemia Miocárdica/epidemiología , Isquemia Miocárdica/metabolismo , Isquemia Miocárdica/fisiopatología , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/metabolismo , Complicaciones Posoperatorias/fisiopatología , Pronóstico , Reoperación , Factores de Riesgo
14.
Biomed Khim ; 56(6): 739-46, 2010.
Artículo en Ruso | MEDLINE | ID: mdl-21395076

RESUMEN

Preparation and stability of a few examples of medical sorbents are described. A simple and practical technique has been developed for sorbent preparation with the low weight synthetic ligands such as amino acids, peptides or oligosaccharides. This approach to sorbent preparation enables the development of the new affine columns generation for medicine and biotechnology to be carried out with ease.


Asunto(s)
Aminoácidos/química , Eliminación de Componentes Sanguíneos/métodos , Oligosacáridos/química , Péptidos/química , Humanos
15.
Bull Exp Biol Med ; 148(2): 275-8, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20027346

RESUMEN

We studied interferon status in children during acute respiratory infections and found that it depends on etiology, course of the disease, and individual features of the organism. The efficiency of IFN inductor anaferon (pediatric formulation) and the possibility of its application in the therapy of children with acute respiratory infections were demonstrated.


Asunto(s)
Antivirales/uso terapéutico , Inductores de Interferón/uso terapéutico , Interferones/sangre , Infecciones del Sistema Respiratorio/sangre , Infecciones del Sistema Respiratorio/tratamiento farmacológico , Enfermedad Aguda , Adolescente , Adulto , Niño , Preescolar , Femenino , Humanos , Lactante , Interferón-alfa/sangre , Interferón gamma/sangre , Masculino , Infecciones del Sistema Respiratorio/virología , Adulto Joven
16.
Bioorg Khim ; 35(3): 311-22, 2009.
Artículo en Ruso | MEDLINE | ID: mdl-19621046

RESUMEN

Two fragments corresponding to the 125-133 and 206-218 sequences of a molecule of the beta(1) adrenoreceptor (autoantibodies to this protein are often found in patients with dilated cardiomyopathy) were synthesized by the solid phase method with the use of Fmoc technology. Two new conformational antigens were prepared by directed (regioselective) and undirected (spontaneous) formation of intramolecular and intermolecular disulfide bridges between the corresponding cysteine residues of the synthesized peptides. One of these antigens consisted of a mixture of disulfide isomers, and another antigen was an isomer with a natural arrangement of S-S bridges. Immunosorbents were obtained by immobilization of the synthesizes antigens on the bromocyanogenactivated sepharose and applied to the removal of autoantibodies in a beta(1)-adrenoreceptor from the blood plasma of patients. We demonstrated that the sorbents on the basis of the conformational antigens were more effective in comparison with those containing linear peptide precursors.


Asunto(s)
Antígenos/química , Disulfuros/síntesis química , Péptidos/síntesis química , Receptores Adrenérgicos beta 1/química , Antígenos/inmunología , Autoanticuerpos/sangre , Autoanticuerpos/aislamiento & purificación , Cardiomiopatía Dilatada/sangre , Cardiomiopatía Dilatada/inmunología , Cromatografía Líquida de Alta Presión , Disulfuros/química , Humanos , Técnicas de Inmunoadsorción , Péptidos/química , Péptidos/inmunología , Estructura Terciaria de Proteína , Receptores Adrenérgicos beta 1/inmunología
17.
Ter Arkh ; 81(5): 50-4, 2009.
Artículo en Ruso | MEDLINE | ID: mdl-19537587

RESUMEN

AIM: To determine distribution of different atherosclerosis risk factors and their correlation with severity of coronary arteries (CA) affection in young men. MATERIAL AND METHODS: A total of 235 males aged 28-45 years were examined with coronarography which detected more than 50% narrowing in one or several major CA in 200 patients (the study group with coronary artery disease), 35 males without coronary stenosis served control. Atherosclerosis risk factors, serum lipids, lipoprotein(a), fibrinogen, C-reactive protein (CRP) were assessed. RESULTS: Smoking and hyperlipidemia (HLE) were registered in the study group more frequently. The level of lipoprotein(a) above 30 mg/dl was recorded in 98 (49%) and 7 (20%) patients of the study and control group, respectively (p = 0.001). As shown by a correlation analysis, the number of affected CA was associated with smoking (r = 0.2; p < 0.001), HLE (r = 0.23; p < 0.001), concentration of total cholesterol (r = 0.23; p < 0.0.001), lipoprotein (a) (r = 0.26; p < 0.001) and CRP (r = 0.26; p < 0.05). The level of lipoprotein(a) in patients with myocardial infarction and occluded artery was significantly higher than in patients without myocardial infarction and non-occlusive CA affection. CONCLUSION: The presence and severity of coronary atherosclerosis in young men with coronary artery disease are associated with smoking, HLE and high concentration of lipoprotein (a).


Asunto(s)
Enfermedad de la Arteria Coronaria/complicaciones , Hiperlipidemias/complicaciones , Lipoproteína(a)/sangre , Isquemia Miocárdica/etiología , Fumar/efectos adversos , Adulto , Factores de Edad , Estudios de Casos y Controles , Enfermedad de la Arteria Coronaria/sangre , Humanos , Hiperlipidemias/sangre , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/sangre , Factores de Riesgo
18.
Bull Exp Biol Med ; 142(5): 587-90, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-17415469

RESUMEN

Efficiency of sorbents for LDL apheresis was compared in vitro. The sorbents based on ion-exchange interaction of the ligand with LDL (Liposorber, DALI) exhibited minimum specificity towards the eliminated component, while immunosorbents (LNP-Lipopak, LDR-TheraSorb) were most efficient. By sorption capacity, the available hemosorbents are inferior to plasmasorbents, which explains low efficiency of the therapy based on single application of hemosorbents especially in patients with consederably increased content of LDL cholesterol.


Asunto(s)
Eliminación de Componentes Sanguíneos/métodos , Técnicas de Inmunoadsorción , Lipoproteínas LDL/aislamiento & purificación , Humanos
19.
Kardiologiia ; 42(7): 47-52, 2002.
Artículo en Ruso | MEDLINE | ID: mdl-12494113

RESUMEN

There are limited data concerning influence of hormone replacement therapy (HRT) on lipid profile in women with type 2 diabetes. Aim of the study was to compare changes of blood lipids during HRT in postmenopausal women with and without type 2 diabetes. Seventy seven women included in the study were assigned to 1 of 4 groups, basing on being diabetic or nondiabetic, and further subdivided into users of estrogen alone (ERT), and of estrogen plus progestin (EPRT). Effect of 6-month ERT (oral estradiol valerate 2 mg/day) and EPRT (oral estradiol valerate 2 mg/day sequentially combined with cyproterone acetate 1 mg/day) on total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides and lipoprotein (a) [Lp(a)] was separately assessed. EPRT and ERT caused decrease in LDL-C by 15% and 12%, and increase in HDL-C by 12% and 13%, respectively, in patients with diabetes (p<0.05 in all cases). LDL-C decreased by 11% and 15%, respectively, in women without diabetes (p<0.05 in all cases). Lp(a) was also reduced 25% with EPRT (p<0.01) and ERT (p<0.05). HDL-C increased 10% (p<0.05) with ERT but remained unchanged with EPRT. In conclusion, changes in all lipid parameters except Lp(a) caused by ERT and EPRT were comparable in postmenopausal women with and without type 2 diabetes.


Asunto(s)
HDL-Colesterol/sangre , LDL-Colesterol/sangre , Diabetes Mellitus Tipo 2 , Terapia de Reemplazo de Estrógeno/métodos , Estrógenos/uso terapéutico , Posmenopausia/fisiología , Congéneres de la Progesterona/uso terapéutico , Triglicéridos/sangre , Anciano , Quimioterapia Combinada , Femenino , Humanos , Persona de Mediana Edad
20.
Kardiologiia ; 42(5): 4-8, 2002.
Artículo en Ruso | MEDLINE | ID: mdl-12494139

RESUMEN

Relationship between level of lipoprotein (a) and presence of ischemic heart disease (IHD) was studied in 191 patients with hypertensive disease (essential hypertension) (112 men, 79 women, age 56.9-/+10.6 years) and 33 patients with normal blood pressure (23 men, 10 women, age 50.8-/+11.0 years). Concentration of lipoprotein (a) in men with hypertension and IHD (n=62) was significantly higher than in men with hypertension without IHD (n=50) (median 19 and 9 mg/dl, respectively, p=0.02). Women with hypertension and with (n=23) and without (n=56) IHD had similar blood levels of lipoprotein (a) (median 13 and 15 mg/dl, respectively, p=0.89). Difference in lipoprotein (a) levels between patients with hypertensive disease and those with normal blood pressure was not significant (median 14 and 10 mg/dl, respectively, p=0.50).


Asunto(s)
Hipertensión/sangre , Lipoproteína(a)/sangre , Isquemia Miocárdica/sangre , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Biomarcadores , Angiografía Coronaria , Interpretación Estadística de Datos , Complicaciones de la Diabetes , Femenino , Humanos , Hipertensión/complicaciones , Técnicas para Inmunoenzimas , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/diagnóstico , Isquemia Miocárdica/diagnóstico por imagen , Estudios Prospectivos , Estudios Retrospectivos , Factores Sexuales , Fumar/efectos adversos
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