RESUMEN
El objetivo de este estudio fue determinar el conocimiento de profesores pertenecientes a establecimientos educacionales particulares subvencionados de Osorno con respecto al trauma dentoalveolar. Estudio de corte transversal, donde se realizó una encuesta presencial o en línea basada en un cuestionario con el fin de determinar el conocimiento sobre trauma dentoalveolar en profesores de enseñanza básica y media, pertenecientes a establecimientos educacionales de la comuna de Osorno, Chile. Los criterios de inclusión fueron, empleo a tiempo completo en el momento de la recolección de datos con al menos 1 año de experiencia docente. La jubilación, docentes con edad mayor o igual a 65 años y la falta de voluntad para participar en la encuesta, fueron considerados como criterios de exclusión. Para evaluar los resultados obtenidos se utilizó la prueba de la t de Student o ANOVA de una vía, con post hoc de Tukey. El nivel de significancia se fijó en p ≤ 0,05. Los profesores tuvieron un promedio de conocimiento sobre trauma dental de 5,0 ±3,0 puntos, de un puntaje máximo posible de 14, sin diferencias estadísticamente significativas entre el nivel de enseñanza donde ejerce la docencia (5,2±2,8, 4,9±3,3 y 4,9±3,3 puntos, p=0,88). El conocimiento sobre traumatismo dentoalveolar entre los profesores es deficiente. Se recomienda implementar programas educativos para el manejo de traumas dentales.
The aim of this study was to determine the knowledge of teachers affiliated to private subsidized schools in Osorno regarding dentoalveolar trauma. Cross-sectional study, where a survey was carried out face-to-face or online, based on a questionnaire to determine the knowledge about dentoalveolar trauma in elementary and middle school teachers, affiliated with educational establishments in the commune of Osorno, Chile. Inclusion criteria were full-time employment at the time of data collection, with at least one year of experience. Retirement, teachers aged 65 or older, and unwillingness to participate in the survey were considered exclusion criteria. The data were analyzed using the R software, Student's t test or one- way ANOVA, Tukey's post hoc. The significance level was set at p < 0.05. Teachers had an average knowledge of dental trauma of 5.0±3.0 points, out of a maximum possible score of 14, without statistically significant differences between the level of education where they teach (5.2±2.8, 4.9±3.3 and 4.9±3.3 points, p = 0.88). Knowledge regarding dentoalveolar traumatism among teachers of private subsidized schools in Osorno is deficient. It is recommended to implement educational programs for the management of dental trauma.
RESUMEN
While understanding the genetic underpinnings of osteogenesis has far-reaching implications for skeletal diseases and evolution, a comprehensive characterization of the osteoblastic regulatory landscape in non-mammalian vertebrates is still lacking. Here, we compared the ATAC-Seq profile of Xenopus tropicalis (Xt) osteoblasts to a variety of non mineralizing control tissues, and identified osteoblast-specific nucleosome free regions (NFRs) at 527 promoters and 6747 distal regions. Sequence analyses, Gene Ontology, RNA-Seq and ChIP-Seq against four key histone marks confirmed that the distal regions correspond to bona fide osteogenic transcriptional enhancers exhibiting a shared regulatory logic with mammals. We report 425 regulatory regions conserved with human and globally associated to skeletogenic genes. Of these, 35 regions have been shown to impact human skeletal phenotypes by GWAS, including one trps1 enhancer and the runx2 promoter, two genes which are respectively involved in trichorhinophalangeal syndrome type I and cleidocranial dysplasia. Intriguingly, 60 osteoblastic NFRs also align to the genome of the elephant shark, a species lacking osteoblasts and bone tissue. To tackle this paradox, we chose to focus on dlx5 because its conserved promoter, known to integrate regulatory inputs during mammalian osteogenesis, harbours an osteoblast-specific NFR in both frog and human. Hence, we show that dlx5 is expressed in Xt and elephant shark odontoblasts, supporting a common cellular and genetic origin of bone and dentine. Taken together, our work (i) unravels the Xt osteogenic regulatory landscape, (ii) illustrates how cross-species comparisons harvest data relevant to human biology and (iii) reveals that a set of genes including bnc2, dlx5, ebf3, mir199a, nfia, runx2 and zfhx4 drove the development of a primitive form of mineralized skeletal tissue deep in the vertebrate lineage.
RESUMEN
Ctenostomes are a group of gymnolaemate bryozoans with an uncalcified chitinous body wall having few external, skeletal characters. Hence, species identification is challenging and their systematics remain poorly understood, even more so when they exhibit an endolithic (boring) lifestyle. Currently, there are four Recent families of endolithic bryozoans that live inside mineralized substrates like mollusk shells. In particular, Penetrantiidae Silén, 1946 has received considerable attention and its systematic affinity to either cheilostomes or ctenostomes has been debated. Species delimitation of penetrantiids remains difficult, owing to a high degree of colonial and zooidal plasticity. Consequently, an additional molecular approach is essential to unravel the systematics of penetrantiids, their phylogenetic placement and their species diversity. We therefore sequenced the mitochondrial (mt) genomes and two nuclear markers of 27 ctenostome species including nine penetrantiids. Our phylogeny supports the Penetrantiidae as a monophyletic group placed as sister taxon to the remaining ctenostomes alongside paludicellids, arachnidioids and terebriporids. The boring family Terebriporidae d'Orbigny, 1847 were previously considered to be among vesicularioids, but our results suggest an arachnidioid affinity instead. Ctenostome paraphyly is supported by our data, as the cheilostomes nest within them. A Multiporata clade is also well supported, including the former victorelloid genus Sundanella. Altogether, this study provides new insights into ctenostome systematics, assists with species delimitation and contributes to our understanding of the bryozoan tree of life.
RESUMEN
Chlorhexidine (CHX) is one of the most widely used antiseptics in the oral cavity due to its high antimicrobial potential. However, many authors have stated that the effect of CHX in nonsurgical periodontal therapy is hampered by its rapid elimination from the oral environment. The aim of this study was to determine the antibacterial efficacy of a new compound of chlorhexidine 0.20% + cymenol (CYM) 0.10% on a multispecies biofilm. For this, an in vitro study was designed using a multispecies biofilm model of Streptococcus mutans, Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis. Quantification of the microbial viability of the biofilm was performed using 5-cyano-2,3-ditolyl tetrazolium-chloride (CTC) to calculate the percentage of survival, and the biofilms were observed using a a confocal laser scanning microscopy (CLSM). It was observed that the bactericidal activity of the CHX + cymenol bioadhesive gel was superior to that of the CHX bioadhesive gel, in addition to higher penetrability into the biofilm. Therefore, there was greater elimination of bacterial biofilm with the new compound of chlorhexidine 0.2% plus cymenol 0.1% in a bioadhesive gel form compared to the formulation with only chlorhexidine 0.2% in a bioadhesive gel form.
RESUMEN
El Hepatoblastoma es el principal tumor maligno primario del hí gado en ni ños. El objetivo de este art ículo es recordar que el Hepatoblastoma se presenta principalmente antes de los 3 a ños de vida, y que siempre que palpemos una masa abdominal, pense- mos que es maligno hasta que no se dem - uestre lo contrario. El paciente del presente caso es un lactante masculino de 15 meses de edad, procedente de Santa B árbara , ingresado en la sala de Lactantes del Hos pital Nacional " Mario Catarino Rivas " ( HNMCR ) el 03 de marzo 2010 , con histo ria de dos meses de evoluci ón de masa en cuadrante superior derecho. Se le realiz ó resecci ón total del tumor y luego se inicio quimioterapia. La biopsia report ó Hepato blastoma patr ón morfol ógico cl ásico (Fetal)...(AU)
