RESUMEN
Presentamos el caso de una paciente en edad reproductiva con dolor abdominal crónico y antecedente de endometriosis pelviana. Los estudios por imágenes muestran masa en topografía apendicular. Se decidió exploración laparoscópica programada y se realizó la resección del tumor apendicular. La anatomía patológica mostró endometriosis del apéndice cecal. Posteriormente se realiza una revisión bibliográfica de esta patología y se hacen consideraciones sobre su frecuencia, presentación clínica, hallazgos intraoperatorios, forma de estudio y posibilidades terapéuticas.
We report the case of a female patient in childbearing age with chronic pain and a history of pelvic endometriosis. The image tests showed the presence of a mass at the level of the cecal appendix. The patient underwent a scheduled diagnostic laparoscopy and the appendiceal tumor was resected. The pathological examination revealed appendiceal endometriosis. We performed a review of the literature and made considerations about its prevalence, clinical presentation, interoperative findings, diagnostic tests and therapeutic management.
Asunto(s)
Humanos , Femenino , Apéndice , Dolor Abdominal , Endometriosis , Endometriosis/complicaciones , Prevalencia , Causalidad , Laparoscopía , Endometriosis/diagnóstico , Informe de Investigación , Dolor Crónico , AnatomíaRESUMEN
SDS-CTAB cat-anionic vesicles are supramolecular aggregates forming complexes with biopolymers and enter the cells via membrane fusion or endocytosis. Different applicative areas exist: gene therapy, drug delivery and nanotechnology. We previously examined the absorption/release of biopolymers from vesicles in solution. Here we evaluate their cytotoxicity in cultured cells; to this end we characterized the vesicles and analyzed their biological effects at cellular and molecular level. At low concentration these vesicles have scarce consequences on normal cell growth; at higher dosage they activate apoptotic death processes, due to membrane damage. In conclusion, the use of these particles in nano-biotechnology represents an actual possibility.
Asunto(s)
Apoptosis/efectos de los fármacos , Compuestos de Cetrimonio/farmacología , Liposomas Unilamelares/farmacología , Análisis de Varianza , Animales , Aniones/química , Western Blotting , Cationes/química , Línea Celular , Supervivencia Celular/efectos de los fármacos , Cetrimonio , Compuestos de Cetrimonio/química , Fragmentación del ADN/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Células HL-60 , Células HeLa , Humanos , Etiquetado Corte-Fin in Situ , Poli(ADP-Ribosa) Polimerasas/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Dodecil Sulfato de Sodio/química , Liposomas Unilamelares/químicaRESUMEN
In this report we investigate the alterations of the dielectric properties of the plasma membrane caused by the infection of cultured fibroblasts with murine polyomavirus. The approach consists in a well-established dielectric spectroscopy technique, electrorotation, which has been successfully used in our laboratory to study the alterations of the plasma membrane of cells exposed to various forms of stress. The response to viral proliferation was time dependent as shown by evaluation of the de novo synthesis of viral DNA. This response was paralleled by gradual damage of the membrane evidenced by alteration of the dielectric parameters, specific capacitance and conductance. The electrorotation results show a reduced effect on the dielectric properties of the membrane when infection is carried out in the presence of a natural oil (MEX). In this case a drastic reduction in viral DNA synthesis was also monitored, thus indicating an antiviral action of this product.
