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2.
PLoS One ; 19(8): e0297716, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39106290

RESUMEN

Magnetic drug delivery systems using nanoparticles present a promising opportunity for clinical treatment. This study explored the potential anti-inflammatory properties of RosA- CrFe2O4 nanoparticles. These nanoparticles were developed through rosmarinic acid (RosA) co-precipitation via a photo-mediated extraction technique. XRD, FTIR, and TEM techniques were employed to characterize the nanoparticles, and the results indicated that they had a cubic spinel ferrite (FCC) structure with an average particle size of 25nm. The anti-inflammatory and antioxidant properties of RosA- CrFe2O4 nanoparticles were evaluated by using LPS-induced raw 264.7 macrophages and a hydrogen peroxide scavenging assay, respectively. The results showed that RosA- CrFe2O4 nanoparticles had moderate DPPH scavenging effects with an IC50 value of 59.61±4.52µg/ml. Notably, these nanoparticles effectively suppressed the expression of pro-inflammatory genes (IL-1ß, TNF-α, IL-6, and iNOS) in LPS-stimulated cells. Additionally, the anti-inflammatory activity of RosA- CrFe2O4 nanoparticles was confirmed by reducing the release of secretory pro-inflammatory cytokines (IL-6 and TNF-α) in LPS-stimulated macrophages. This investigation highlights the promising potential of Phyto-mediated CrFe2O4-RosA as an anti-inflammatory and antioxidant agent in biomedical applications.


Asunto(s)
Antiinflamatorios , Antioxidantes , Cinamatos , Depsidos , Compuestos Férricos , Nanopartículas de Magnetita , Ácido Rosmarínico , Depsidos/farmacología , Depsidos/química , Animales , Ratones , Antioxidantes/farmacología , Antioxidantes/química , Antiinflamatorios/farmacología , Antiinflamatorios/química , Cinamatos/química , Cinamatos/farmacología , Compuestos Férricos/química , Compuestos Férricos/farmacología , Nanopartículas de Magnetita/química , Células RAW 264.7 , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Lipopolisacáridos/farmacología , Citocinas/metabolismo , Tamaño de la Partícula
3.
Chem Biodivers ; : e202401060, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38949232

RESUMEN

Research interest in examining Elaeagnus angustifolia's potential as a source of anti-inflammatory and antioxidant agents has grown as a result of the plant's endorsement as a rich source of bioactive chemicals with promising anti-inflammatory and antioxidant activity. In this study, zinc oxide (Fe0.25-ZnO) bimetallic nanoparticles (E.ang-Fe0.25-ZnO NPs) were synthesized using an aqueous extract of Elaeagnus angustifolia. Synthesized Fe0.25-ZnO nanoparticles were characterized by FTIR and XRD. The anti-inflammatory and antioxidant activities were investigated in LPS-stimulated RAW 264.7 macrophages using RT-PCR and ELISA techniques for antioxidant- and inflammation-related genes. The concentration of 39.6 µg/ml of E.ang-Fe0.25-ZnO NPs demonstrated a significant anti-inflammatory activity by suppressing the mRNA levels of TNF-α and IL-6 by 88.3 %±1.9 and 93.6 %±0.1, respectively, compared to LPS-stimulated cells. This was confirmed by the significant reduction of TNF-α and IL-6 secretion levels from 95.2 and 495.6 pg/ml in LPS-stimulated cells to 5.6 and 26.5 pg/ml in E.ang-Fe0.25-ZnO treated group. In addition, E.ang-Fe0.25-ZnO NPs nanoparticles treatment significantly enhanced the expression of antioxidant-related genes, SOD and CAT. Together, our results proved that phyto-mediated Fe0.25-ZnO nanoparticles using Elaeagnus angustifolia have great potential in biomedical applications such as anti-inflammatory and antioxidant.

4.
Sci Rep ; 14(1): 13987, 2024 06 17.
Artículo en Inglés | MEDLINE | ID: mdl-38886466

RESUMEN

The nuclear receptor-related factor 1 (Nurr1), an orphan nuclear receptor in microglia, has been recognized as a major player in attenuating the transcription of the pro-inflammatory genes to maintain CNS homeostasis. In this study, we investigate Nurr1 trans-repression activity by targeting this receptor with one of the indole derivatives 3-Indole acetic acid hydrazide (IAAH) loaded onto zinc iron oxide (ZnFe2O4) NPs coated with PEG. XRD, SEM, FTIR, UV-Vis spectroscopy, and DLS were used to characterize the synthesized IAAH-NPs. The anti-inflammatory properties of IAAH-NPs on LPS-stimulated SimA9 microglia were assayed by measuring pro-inflammatory cytokine gene expressions and protein levels using RT-PCR and ELISA, respectively. As a result, IAAH-NPs showed an ability to suppress pro-inflammatory genes, including IL-6, IL-1ß, and TNF-α in LPS-stimulated SimA9 via targeting Nurr1. The current study suggests that ZnFe2O4 NPs as a delivery system can increase the efficiency of cellular uptake and enhance the IAAH ability to inhibit the pro-inflammatory cytokines. Collectively, we demonstrate that IAAH-NPs is a potential modulator of Nurr1 that combines nanotechnology as a delivery system to suppress neuroinflammation in CNS which opens a window for possible ambitious neuroprotective therapeutic approaches to neuro disorders.


Asunto(s)
Microglía , Nanopartículas , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares/metabolismo , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares/genética , Microglía/efectos de los fármacos , Microglía/metabolismo , Animales , Ratones , Nanopartículas/química , Línea Celular , Indoles/farmacología , Indoles/química , Regulación de la Expresión Génica/efectos de los fármacos , Compuestos Férricos/química , Compuestos Férricos/farmacología , Lipopolisacáridos/farmacología , Citocinas/metabolismo , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Ligandos , Antiinflamatorios/farmacología , Antiinflamatorios/química , Ácidos Indolacéticos
5.
PLoS One ; 19(4): e0297217, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38635692

RESUMEN

This study focuses on isolated thermophilic Bacillus species' adaptability and physiological diversity, highlighting their ecological roles and potential industrial applications. We specifically investigated their capacity to thrive in extreme conditions by examining their environmental tolerances and adaptations at the metabolic and genetic levels. The primary objective is to evaluate the suitability of these species for biotechnological applications, considering their resilience in harsh environments. We conducted a comparative analysis of the environmental adaptability parameters for various Bacillus species. This included examining growth temperature ranges, pH tolerance, oxygen requirements, carbohydrate fermentation patterns, colony morphology, enzymatic activities, and genetic properties. Controlled laboratory experiments provided the data, which were then analyzed to determine patterns of adaptability and diversity. The research revealed that Bacillus species could endure temperatures as high as 73°C, with a generally lower growth limit at 43°C. However, strains TBS35 and TBS40 were exceptions, growing at 37°C. Most strains preferred slightly alkaline conditions (optimal pH 8), but TBS34, TBS35, and TBS40 exhibited adaptations to highly alkaline environments (pH 11). Oxygen requirement tests classified the species into aerobic, anaerobic, and facultative aerobic categories. Genetic analysis highlighted variations in DNA concentrations, 16s rRNA gene lengths, and G+C content across species. Although glucose was the primary substrate for carbohydrate fermentation, exceptions indicated metabolic flexibility. The enzymatic profiles varied, with a universal absence of urease and differences in catalase and oxidase production. Our findings underscore thermophilic Bacillus species' significant adaptability and diversity under various environmental conditions. Their resilience to extreme temperatures, pH levels, varied oxygen conditions, and diverse metabolic and genetic features emphasize their potential for biotechnological applications. These insights deepen our understanding of these species' ecological roles and highlight their potential industrial and environmental applications.


Asunto(s)
Bacillus , ARN Ribosómico 16S/genética , Calor , Oxígeno , Carbohidratos , Filogenia
6.
Nanoscale Adv ; 6(6): 1611-1642, 2024 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-38482039

RESUMEN

Iron oxide nanozymes (IONzymes) are a class of magnetic nanoparticles that mimic the enzymatic activity of natural enzymes. These particles have received significant attention in recent years due to their unique properties, such as high stability, tunable magnetic responsiveness, and ability to act as biocatalysts for various chemical reactions. In this review, we aim to provide an overview of the production methods of magnetic nanozymes, including chemical, physical, and biological synthesis. The structure and design of magnetic nanozymes are also discussed in detail, as well as their applications in various fields such as biomedicine and environmental science. The results of various studies and the latest advances in the field of magnetic nanozymes are also discussed. This review provides valuable insights into the current state of magnetic nanozymes and highlights their potential for further development and application in various fields.

7.
Biomed Rep ; 20(3): 36, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38343659

RESUMEN

The use of plants for nanoparticle (NP) synthesis, grounded in green chemistry principles, is an environmentally friendly and economically viable approach. In the present study, the leaf extract of Elaeagnus angustifolia L. was used as a biosynthetic agent to generate bimetallic zinc oxide NPs. The present study investigated the effect of ZnO NPs on anti-angiogenesis and cell migration. Various bimetallic NPs, including zinc-iron oxide and nickel-zinc oxide, underwent characterization through Fourier-transform infrared spectroscopy and X-ray Diffraction within the 25-65˚ range. Confirmation of NP formation was determined by identifying the surface plasmon resonance peak. MTT assay was used to determine the cytotoxic properties of E. angustifolia L. extracts, ZnO NPs and associated metals in MCF-7 breast cancer cells. The plant extract demonstrated antiproliferative effects at 200 µg/ml, whereas E. ang-Fe2ZnO4 NPs showed varying cytotoxic effects based on concentration. The rat aortic ring and cell migration assays illuminated anti-angiogenic attributes, with the E. ang-Fe2ZnO4 NPs blocking blood vessel development entirely at 100 µg/ml, implying profound anti-angiogenic efficacy. Therefore, E. ang-Fe2ZnO4 NPs may serve a role in antiangiogenic therapy.

8.
PLoS One ; 19(2): e0298200, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38358999

RESUMEN

The Wadi Hanifah, a crucial aquatic ecosystem, has unfavorable consequences from natural occurrences and human activities. Recognizing the critical need for sustainable water management, this study provides an in-depth evaluation of wadi water quality. A comprehensive assessment was conducted, analyzing physical properties (temperature, pH, electrical conductivity, turbidity, color, and odor), chemical constituents (nitrogen compounds, ion concentrations, heavy metals), and bacterial diversity. The study found significant temperature fluctuations, particularly in sun-exposed or stagnant water areas. The water exhibited slight alkalinity and variable electrical conductivity and turbidity, indicating differing pollution levels. High ammonia and heavy metal concentrations suggested organic and industrial contamination, respectively. In addition, the prevalent fecal-indicator bacteria pointed to possible sewage or agricultural runoff. The research highlights the complex interplay of natural and anthropogenic factors affecting Wadi Hanifah's water quality. It emphasizes the need for location-specific environmental management strategies focusing on pollution control and conservation to safeguard the wadi's ecological health. This study provides vital insights for effective water resource management in Wadi Hanifah, serving as a model for similar ecosystems.


Asunto(s)
Metales Pesados , Contaminantes Químicos del Agua , Humanos , Calidad del Agua , Ecosistema , Monitoreo del Ambiente , Contaminantes Químicos del Agua/análisis , Metales Pesados/análisis
9.
Chem Biodivers ; 21(5): e202301739, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38243670

RESUMEN

Newly, green metallic-nanoparticles (NPs) have received scientists' interest due to their wide variable medicinal applications owned to their economical synthesis and biologically compatible nature. In this study, we used rosmarinic acid (RosA) to prepare Cu0.5Zn0.5FeO4 NPs and later encapsulated them using PEG polymer. Characterization of NPs was done using the XRD method and SEM imaging. Further, we explored the encapsulated NPs for anti-inflammatory properties by downregulating the expression of pro-inflammatory cytokines mRNA in LPS-stimulated Raw 264.7 cells. Besides, employing DPPH, NO and ABTS radical scavenging assays to examine the antioxidant activity of the synthesized Cu0.5Zn0.5FeO4 NPs. Cu0.5Zn0.5FeO4 NPs revealed moderate antioxidant activity by scavenging DPPH and nitric oxide. We demonstrated that the NPs showed high potential anti-inflammatory activity by suppressing the mRNA and protein levels of pro-inflammatory cytokines in a dose-dependent manner, in LPS-induced Raw 264.7 cells. To our best knowledge, this is the first report where RosA was found to be a suitable phyto source for the green synthesis of Cu0.5Zn0.5FeO4 NPs and their in vitro anti-inflammatory and antioxidant effects. Taken together, our findings suggest that the RosA is a green resource for the eco-friendly synthesis of Cu0.5Zn0.5FeO4/PEG NPs, which further can be employed as a novel anti-inflammatory therapeutic agent.


Asunto(s)
Antiinflamatorios , Antioxidantes , Cinamatos , Cobre , Depsidos , Lipopolisacáridos , Nanopartículas del Metal , Ácido Rosmarínico , Ratones , Animales , Depsidos/farmacología , Depsidos/química , Células RAW 264.7 , Cinamatos/química , Cinamatos/farmacología , Antioxidantes/farmacología , Antioxidantes/química , Cobre/química , Cobre/farmacología , Lipopolisacáridos/antagonistas & inhibidores , Lipopolisacáridos/farmacología , Antiinflamatorios/química , Antiinflamatorios/farmacología , Nanopartículas del Metal/química , Zinc/química , Zinc/farmacología , Picratos/antagonistas & inhibidores , Compuestos de Bifenilo/antagonistas & inhibidores , Compuestos de Bifenilo/química , Óxido Nítrico/metabolismo , Óxido Nítrico/biosíntesis , Óxido Nítrico/antagonistas & inhibidores , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Ácidos Sulfónicos/antagonistas & inhibidores , Ácidos Sulfónicos/química , Relación Dosis-Respuesta a Droga
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