Arthrospira platensis transglutaminase derived antioxidant peptide-packed electrospun chitosan/ poly (vinyl alcohol) nanofibrous mat accelerates wound healing, in vitro, via inducing mouse embryonic fibroblast proliferation.

In this present study, we have carried out the antioxidant function of transglutaminase (TG) identified from Arthrospira platensis (Ap) transcriptome. The antioxidant peptide ML11 (MLRSIGIPARL) has been predicted from the transglutaminase core domain and the peptide's free radical scavenging potential was evaluated and it shows that it functions on a dose dependent manner. The ML11 peptide cell toxicity was analysed in the human blood leucocytes which resulted no cytotoxic activity in any of the cell population. Moreover, the nanofibre mat encapsulated with antioxidant peptide ML11 was prepared by electrospinning technique. The antioxidant peptide ML11 encapsulated mat showed increase in fibre diameter compared to the chitosan polyvinyl alcohol blended mat. The change in the crystalline behaviour of both chitosan and polyvinyl alcohol polymer to the amorphous nature was determined by X-ray diffraction at the broad band between 20 and 30° (2θ°). FTIR revealed the functional groups which present in the polymer as well as the interaction between their components of chitosan (CS) and polyvinyl alcohol (PVA). The fibre retains the antioxidant activity due to the peptide encapsulated by scavenging the intracellular ROS that was confirmed by flowcytometry and fluorescence microscopy. The ML11 peptide encapsulated mat showed no cytotoxicity in the NIH-3T3 mouse embryonic fibroblast cells. Also, ML11 peptide encapsulated fibre showed potential wound healing activity in NIH-3T3 cells. Taken altogether, the study indicates that the wound healing potential of the ML11 peptide encapsulated nano fibre mat may be used as biopharmaceutical drug.

Assessment of in-vivo biocompatibility evaluation of phytogenic gold nanoparticles on Wistar albino male rats.

Nanomedicine is an interdisciplinary approach that involves toxicology and other medicinal applications. Gold nanoparticles (AuNPs) may serve as a promising model to address the size and shape-dependent biological response because they show good biocompatibility. This study is to prepare phytosynthesis AuNPs from ten different Cassia sp. Among them, the aqueous leaf extract of C. roxburghii produced greater efficient and stable AuNPs. The AuNPs were optimised for different physicochemical conditions. Highly stable AuNPs were synthesised at pH 7.0, 37°C, 1.0 ml of C. roxburghii leaf extract and 1.0 mM concentration of HAuCl4 with the particle size of ∼50 nm and these AuNPs were stable up to 12 months. To determine the safety profile of AuNPs in-vivo, the nanoparticles were injected intravenously into male Wistar albino rats in varying dosages. The authors noticed no significant difference in body weights, haematological and biochemical parameters and the histopathological sections of all vital organs. Highest accumulation was seen in spleen and least in brain. The authors' results show that the AuNPs were biocompatible and did not produce any adverse or abnormalities in-vivo. The implications of the bioaccumulation of AuNPs need to be further studied to rule out any adverse effects on long-term exposure.

Efficacy of mycosynthesised AgNPs from Earliella scabrosa as an in vitro antibacterial and wound healing agent.

The silver nanoparticles (AgNPs) with their unique chemical and physical properties are proving as a new therapeutical agent. In the present study, the AgNPs synthesised from an aqueous extract of a macrofungus, Earliella scabrosa, were characterised by field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDX), high-resolution transmission electron microscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and further evaluate for its in vitro antibacterial and wound healing efficacy. The mycosynthesised AgNPs exhibited the surface plasmon resonance peak at 410 nm with good stability over a period of a month. The FESEM and EDX analyses revealed the spherical-shaped AgNPs of an average size of 20 nm and the presence of elemental Ag, respectively. The XRD pattern showed the crystalline nature of AgNPs. The FTIR spectra confirmed the conversion of Ag+ ions to AgNPs due to reduction by biomolecules of macrofungus extract. The mycosynthesised AgNPs showed effective antibacterial activity against two Gram-positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The pathogens were highly sensitive to AgNPs, whereas less sensitive to AgNO3. The mycosynthesised AgNPs showed significant wound healing potential with 68.58% of wound closure.