Exploring pectin from ripe and unripe Banana Peel: A novel functional fat replacers in muffins.

The study addresses global fruit waste concerns in the food industry by extracting pectin from both ripe and unripe banana peels at varying pH levels and time intervals using hydrochloric acid. The best results were observed for unripe banana peel pectin at pH 1.5 and 250 min exhibiting a yield of 16.46% and favorable characteristics. In muffin development, seven treatments (M0, M1, M2, M3, M4, M5 and M6) are prepared and analyzed for morphology, nutritional content, and sensory parameters. The M4 treatment, utilizing pectin from unripe banana peel at pH 1.5 and 250 min, displays superior qualities with reduced peroxide value, free fatty acids, percent moisture loss, and hardness. Sensory evaluations indicate high acceptability due to lower fat content. In conclusion, the extraction of pectin from unripe banana peels proves promising as a fat replacer in bakery items, maintaining muffin quality while addressing fruit waste challenges in the food industry.

Environmentally relevant concentrations of nickel and imidacloprid induce reproductive toxicity in earthworm (Eisenia fetida fetida).

The current research investigates individual and combined toxicity effects of nickel (Ni) and imidacloprid (IMI) on earthworm species Eisenia fetida fetida. Employing standardized toxicity parameters, we assessed the impact of environmentally relevant concentrations (ERC) of Ni, IMI, and their mixtures on key biomarkers and reproductive fitness of earthworms. Our findings reveal concentration-dependent responses with discernible adverse effects on physiological parameters. The ERC obtained for Ni was 0.095 ppm, and for imidacloprid was 0.01 ppm. Two concentrations (ERC and 1/5th) of both toxicants (individually and in combinations) were further given for 14 days, and parameters like avoidance behaviour, antioxidants, histology, and metabolomic profile were observed. The behaviour of earthworms was noted, where at 24-48 h, it was found to be in control soil, while later, at 72-96 h, they migrated to toxicants-treated soil. Levels of antioxidants (superoxide dismutase, catalase, reduced glutathione, ascorbic acid), lipid peroxidation, and lactate dehydrogenase were elevated in the testis, spermatheca, ovary, and prostate gland in a high concentration of Ni + IMI. Histological studies showed more vacuolization and disruption of epithelium that was increased in the prostate gland of the Ni + IMI high group, decreased number of spermatids, and damaged cell architecture was noted in testis and spermatheca of the Ni + IMI high group. The highest number of metabolites was found in Ni exposed group (181), followed by IMI (131) and Control (125). Thus, this study sheds light on the ecotoxicological effects of combinational exposure of these contaminants on an essential soil-dwelling organism, where IMI was more toxic than Ni, and both toxicants decreased earthworm reproductive fecundity.

FUS3: Orchestrating soybean plant development and boosting stress tolerance through metabolic pathway regulation.

Soybean research has gained immense attention due to its extensive use in food, feedstock, and various industrial applications, such as the production of lubricants and engine oils. In oil crops, the process of seed development and storage substances accumulation is intricate and regulated by multiple transcription factors (TFs). In this study, FUSCA3 (GmFUS3) was characterized for its roles in plant development, lipid metabolism, and stress regulation. Expressing GmFUS3 in atfus3 plants restored normal characteristics observed in wild-type plants, including cotyledon morphology, seed shape, leaf structure, and flower development. Additionally, its expression led to a significant increase of 25% triacylglycerols (TAG) and 33% in protein levels. Transcriptomic analysis further supported the involvement of GmFUS3 in various phases of plant development, lipid biosynthesis, lipid trafficking, and flavonoid biosynthesis. To assess the impact of stress on GmFUS3 expression, soybean plants were subjected to different stress conditions, and the its expression was assessed. Transcriptomic data revealed significant alterations in the expression levels of approximately 80 genes linked to reactive oxygen species (ROS) signaling and 40 genes associated with both abiotic and biotic stresses. Additionally, GmFUS3 was found to regulate abscisic acid synthesis and interact with nucleoside diphosphate kinase 1, which is responsible for plant cellular processes, development, and stress response. Overall, this research sheds light on the multifaceted functions of GmFUS3 and its potential applications in enhancing crop productivity and stress tolerance.

A randomized controlled trial of sweet basil leaf powder-enriched cookies for anemia management in adolescent girls.

The study aimed to evaluate the effectiveness of sweet basil leaf powder as a natural source of iron for the treatment of anemia in adolescent girls. Purposive sampling technique of two-stage sampling; part of the nonprobability sampling approach. Out of 2400 approached adolescent girls, 1645 agreed to participate and their nutritional status was assessed. Of these, 89.95% had clinical signs and symptoms of anemia, and 59.79% were found to be anemic based on Hb levels. From the anemic group, 65.18% were randomly selected to receive either B0 (Control), B1 (12.699 g FeSO4.7H2O/100 g), and B3 (16 g SBLP/100 g) cookies for 4 months. At the end of the intervention, the assessment of nutritional status, complete blood count, serum iron, serum ferritin, serum total iron-binding capacity (TIBC), and transferrin saturation was explored. Hematological parameters such as Hb, Hct, TIBC, MCV, MCH, MCHC, serum iron, and serum ferritin were significant (p ≤ .05). The result showed that the serum Fe was highest in group B3 while a significant decline was noted for group B0. Serum ferritin for B1 was better than B3. The entire treatment for transferrin saturation showed a highly significant increasing trend in B3 and B1, regardless of the control. TIBC levels raised in the control group while in all other treatments, it declined. The study demonstrated that SBLP-fortified cookies can be an effective treatment option for anemia, as evidenced by significant improvements in key hematological parameters.

Halogenated Secondary Metabolites from Higher Plants: Potent Drug Candidates for Chikungunya Using in silico Approaches.

Chikungunya virus (CHIKV) causes a debilitating fever and joint pain, with no specific antiviral treatment available. Halogenated secondary metabolites from plants are a promising new class of drug candidates against chikungunya, with unique properties that make them effective against the virus. Plants produce these compounds to defend themselves against pests and pathogens, and they are effective against a wide range of viruses, including chikungunya. This study investigated the interactions of halogenated secondary metabolites with nsP2pro, a therapeutic target for CHIKV. A library of sixty-six halogenated plant metabolites screened previously for ADME properties was used. Metabolites without violation of Lipinski's rule were docked with nsP2pro using AutoDock Vina. To find the stability of the pipoxide chlorohydrin-nsP2pro complex, the GROMACS suite was used for MD simulation. The binding free energy of the ligand-protein complex was computed using MMPBSA. Molecular docking studies revealed that halogenated metabolites interact with nsP2pro, suggesting they are possible inhibitors. Pipoxide chlorohydrin showed the greatest affinity to the target. This was further confirmed by the MD simulations, surface accessible area, and MMPBSA studies. Pipoxide chlorohydrin, a halogenated metabolite, was the most potent against nsP2pro in the survey.

An investigation on antimicrobial and anticancer competence of macro red algae under in-vitro condition.

The purpose of this study was to look into the proximate parameters (moisture, ash, total fat, protein, and total carbohydrate), mineral composition (Fe, Cu, Mg, and Zn), antimicrobial as well as cytotoxic (anticancer) properties of extracts from the marine red macro algae Gracilaria corticata, Chondrus ocellatus, and Posphyra perforata against a few prevalent microbial pathogens (Salmonella typhi, Streptococcus pneumoniae, Corynebacterium diphtheriae, Clostridium tetani, and Treponema pallidum as well as fungal pathogens such as Candida albicans, Aspergillus niger, and Cryptococcus neoformans) and two cancerous cell lines (HeLa and MCF7). The dry biomass of these red algae biomass contains considerable valuable proximate parameters and minerals. The diffusion technique on agar wells was used to evaluate the antimicrobial properties of these test red algae methanol and hexane extract; MTT assay was used to evaluate the cytotoxic effects of the methanol and hexane extracts on each cancer cell line. The methanol extracts demonstrated significant antimicrobial activity against most of the tested pathogenic organisms. Mortality of cells was effectively caused by methanol extract and it followed by hexane extract at increased dosage 10 mg mL-1. The MTT assay revealed that the methanol extract of the red algae was considerably cytotoxic to HeLa and MCF7 cells, accompanied by the hexane extract in a dose-dependent manner. These findings suggest that the methanol extract of these red algae may contain bioactive compounds with antimicrobial and anticancer properties, which could be studied for future use in the discovery of new drugs from marine ecosystems.

Strategies of plants to overcome abiotic and biotic stresses.

In their environment, plants are exposed to a multitude of abiotic and biotic stresses that differ in intensity, duration and severity. As sessile organisms, they cannot escape these stresses, but instead have developed strategies to overcome them or to compensate for the consequences of stress exposure. Defence can take place at different levels and the mechanisms involved are thought to differ in efficiency across these levels. To minimise metabolic constraints and to reduce the costs of stress defence, plants prioritise first-line defence strategies in the apoplastic space, involving ascorbate, defensins and small peptides, as well as secondary metabolites, before cellular processes are affected. In addition, a large number of different symplastic mechanisms also provide efficient stress defence, including chemical antioxidants, antioxidative enzymes, secondary metabolites, defensins and other peptides as well as proteins. At both the symplastic and the apoplastic level of stress defence and compensation, a number of specialised transporters are thought to be involved in exchange across membranes that still have not been identified, and information on the regeneration of different defence compounds remains ambiguous. In addition, strategies to overcome and compensate for stress exposure operate not only at the cellular, but also at the organ and whole-plant levels, including stomatal regulation, and hypersensitive and systemic responses to prevent or reduce the spread of stress impacts within the plant. Defence can also take place at the ecosystem level by root exudation of signalling molecules and the emission of volatile organic compounds, either directly or indirectly into the rhizosphere and/or the aboveground atmosphere. The mechanisms by which plants control the production of these compounds and that mediate perception of stressful conditions are still not fully understood. Here we summarise plant defence strategies from the cellular to ecosystem level, discuss their advantages and disadvantages for plant growth and development, elucidate the current state of research on the transport and regeneration capacity of defence metabolites, and outline insufficiently explored questions for further investigation.

An assessment of metal absorption competence of indigenous metal tolerant bacterial species- an in-vitro study.

Heavy metals pose a serious global threat to the environment. Hence, removing hazardous metals from soil samples has become complicated over the past few years. The current work looked into the remediation of heavy metals from aqueous solutions using a bacterial community and a unique bacterium obtained from metal-contaminated soil. In this investigation, the isolates of Bacillus anthracis A1-7, Bacillus. thuringiensis A1-3, Bacillus. cereus A1-5, and Pseudomonas aeruginosa A-33 actively demonstrated metal tolerances to various tested metals. Furthermore, an in-vitro biosorption study was performed under ideal concentration. The bacterial consortia achieved the highest biosorption effectiveness for Cu & Zn, 92.7% and 90.3%, respectively. When compared with a single bacterium, the group exhibited inferior Pb biosorption (86%). Since then, P. aeruginosa A33 has had the highest Pb biosorption. Finally, a bacterial consortium has devised an intriguing strategy for eliminating Cu and Pb from the polluted medium. P. aeruginosa A33 was found to be a mighty microbe that extracts Zn from polluted water. This metal-tolerant bacterium also exhibited specific proportions of selective commercially available antibiotics, which were analyzed using the Multiple Antibiotic Resistance (MAR) Index. In conclusion, these findings indicated that bacterial consortia composed of four bacterial isolates can remove metals from a metal-polluted medium.

Conversion of metal-enriched magnetite mine tailings into suitable soil for vegetation by phytoremediation process with Bougainvillaea glabra under the influence of Thiobacillus ferroxidance.

Magnetite mining is a significant contributor to land deterioration as well as HM-based soil contamination. The characteristics of magnetite mine tailing were examined in the present study, in addition to the positive and sustainable restoration strategy with Bougainvillaea glabra under the influence of Thiobacillus ferroxidance. The traits of test soil analysis findings demonstrated that the majority of the parameters exceeded the allowable limits (For instance: HMs such as Cr, Cu, Zn, Pb, Fe, and Co were found to be 208 ± 2.3, 131.43 ± 1.6, 185.41 ± 3.3, 312 ± 5.11, 956 ± 5.3, and 26.89 ± 2.43 mg kg-1 respectively). T. ferroxidance exhibited impressive HMs tolerance for as much as 800 g mL-1 concentrations of Cr, Cu, Zn, Pb, Fe, and Co. To prevent HMs toxic effects, the HMs contents in test soil were decreased by diluting with normal soil in the ratios of Ex-3 and Ex-2. A typical greenhouse study was carried out to assess the phytoremediation ability of B. glabra across six setups for experiments (Ex-1 to Ex-6). According to the findings of this research, the HMs tolerant T. ferroxidance from Ex-3 and Ex-2 had an outstanding impact on the growth, biomolecules level (such as chlorophylls: 65.84 & 41.1 mg g-1, proteins: 165.1 & 151.1 mg g-1, as well as carbohydrates: 227.4 & 159.3 mg g-1) as well as phytoremediation potential of B. glabra on magnetite mine soil. These findings indicated that a mixture of B. glabra as well as T. ferroxidance might serve as a valuable sustainable agent for removing HMs from contaminated soil.

Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water.

Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: "avoidance" by efficient sodium and chloride exclusion at the roots, and "acclimation" by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance.

An assessment of ozone risk for date palm suggests that phytotoxic ozone dose nonlinearly affects carbon gain.

Tropospheric ozone (O3) is a significant phytotoxic air pollutant that has a negative impact on plant carbon gain. Although date palm (Phoenix dactylifera L.) is a globally important crop in arid or semi-arid regions, so far O3 risk assessment for this species has not been reported. This study estimated leaf- and plant-level photosynthetic CO2 uptake for understanding how elevated levels of O3 affects date palm biomass growth. Ozone risks to date palm plants were assessed based on exposure- (AOT40) or flux-based indices (Phytotoxic Ozone Dose, PODy, where y is a threshold of uptake). For this purpose, plants were exposed to three levels of O3 [ambient air, AA (45 ppb as daily average); 1.5 × AA; 2.0 × AA] for 92 days in an O3 Free-Air Controlled Exposure facility. According to the model simulations, the negative effects of O3 on plant-level net photosynthetic CO2 uptake were attributed to reduced gross photosynthetic carbon gain and increased respiratory carbon loss. Season-long O3 exposure and elevated temperatures promoted the negative O3 effect because of a further increase of respiratory carbon loss, which was caused by increased leaf temperature due to stomatal closure. POD1 nonlinearly affected the photosynthetic CO2 uptake, which was closely related to the variation of dry mass increment during the experiment. Although the dose-response relationship suggested that a low O3 dose (POD1 < 5.2 mmol m-2) may even positively affect photosynthetic CO2 uptake in date palms, stomatal O3 uptake at the current ambient O3 levels has potentially a negative impact on date palm growth. The results indicate 5.8 mmol m-2 POD1 or 21.1 ppm h AOT40 as critical levels corresponding to a 4% reduction of net CO2 uptake for date palm, suggesting that this species can be identified as a species moderately sensitive to O3.

Synergism and toxicity of iron nanoparticles derived from Trigonella foenum-graecum against pyrethriod treatment in S. litura and H. armigera (Lepidoptera: Noctuidae).

The tobacco cutworm, Spodoptera litura and cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) are important pests of various agricultural crops that cause sevier economic loses throughout the world. Indiscriminate and frequent use of insecticide may lead to development of resistance in these pests. Nanotechnology has given an alternative to manage and overcome insecticide resistance for pest management strategies. In the present study the iron nanoparticles derived from Trigonella foenum-graecum leaf extract (FeNPs) was investigated for its ecofriendly management of pyrethroid resistance in two lepidopteron pest species at 24 h, 48 h and 72 h post treatment. The result showed high mortality (92.83% and 91.41%) of S. litura and H. armigera at 72 h treatment upon FeNPs and fenvalerate (Fen + FeNPs) teratment. Probit analysis revealed high LC50 upon Fen + FeNPs treatment (130.31 and 89.32 mg/L) with a synergism ratio of 1.38 and 1.36. Antifeedant activity of six dofferent concentration of FeNPs revelaed increased antifeedant activity with respect to increasing concentration of nanoparticles ranging from 10 to 90% and 20-95% againt both insects (p<0.05). Detoxification activity of carboxylesterase was elevated at 630 µmol/mg protein/min (p<0.05) in fenvalerate treatment, whereas decreased activity was found (392umole/mg protein/min) in FeNPs and Fen + FeNPs treatment (P<0.001). GST and P450 activity was also increased in fenvalerate treatment, whereas decreased activity was observed in FeNPs and Fen + FeNPs. Esterase isoenzyme banding pattern revealed four bands in fenvalerate treatment and two bans (E3 and E4) in Fen + FeNPs combination. Hence the present study concludes that T. foenum-graecum synthesized iron nanoparticles could be an effective alternate for ecofriendly management of S. litura and H. armigera.

Organic gelatin-coated ZnNPs for the production of biodegradable biopolymer films.

Petroleum-based polymers have raised significant environmental concerns. It is critical to create compostable, good biocompatibility, and nontoxic polymers to replace petroleum-based polymers. Thus, this research was performed to extract the gelatin from fish waste cartilage and coated it over the surface of spherical shaped pre-synthesized ZnNPs along with a suitable plasticizer to produce the biodegradable film. The presence of gelatin on the surface of ZnNPs was first confirmed using UV-visible spectrophotometers, as well as the characteristic functional groups involved in the coating were investigated using Fourier-Transform Infrared Spectroscopy (FTIR). The morphological appearance of gelatin coated ZnNPs was ranged from 41.43 to 52.31 nm, the shape was found as platonic to pentagonal shape, and the fabricated film was observed through Scanning Electron Microscope (SEM). The thickness, density, and tensile strength of fabricated film were found to be 0.04-0.10 mm, 0.10-0.27 g/cm3, and 31.7 kPa. These results imply that the fish waste cartilage gelatin coated ZnNPs-based nanocomposite can be used for film preparation as well as a wrapper for food and pharmaceutical packaging.

Mixed pollutants adsorption potential of Eichhornia crassipes biochar on Manihot esculenta processing industry effluents.

The starch is one of the most essential food stuff and serves as a raw material for number of food products for the welfare of human. During the production process enormous volume of effluents are being released into the environment. In this regard, this study was performed to evaluate the physicochemical traits of Manihot esculenta processing effluent and possible sustainable approach to treat this issue using Eichhornia crassipes based biochar. The standard physicochemical properties analysis revealed that the most the parameters (EC was recorded as 4143.17 ± 67.12 mhom-1, TDS: 5825.62 ± 72.14 mg L-1, TS: 7489.21 ± 165.24 mg L-1, DO: 2.12 ± 0.21 mg L-1, BOD 2673.74 ± 153.53 mg L-1, COD: 6672.66 ± 131.21 mg L-1, and so on) were beyond the permissible limits and which can facilitate eutrophication. Notably, the DO level was considerably poor and thus can support the eutrophication. The trouble causing E. crassipes biomass was used as raw material for biochar preparation through pyrolysis process. The temperature ranging from 250 to 350 °C with residence time of 20-60 min were found as suitable temperature to provide high yield (56-33%). Furthermore, 10 g L-1 concentration of biochar showed maximum pollutant adsorption than other concentrations (5 g L-1 and 15 g L-1) from 1 L of effluent. The suitable temperature required to remediate the pollutants from the effluent by biochar was found as 45 °C and 35 °C at 10 g L-1 concentration. These results conclude that at such optimized condition, the E. crassipes effectively adsorbed most of the pollutants from the M. esculenta processing effluent. Furthermore, such pollutants adsorption pattern on biochar was confirmed by SEM analysis.

Eichhornia crassipes biochar aided pollutants sorption competence of multi-metal tolerant fungi species on South Pennar river.

The number of studies about the use of efficient techniques to treat contaminated water bodies has increased in recent years. The use of bioremediation method for the reduction of contaminants from aqueous system is receiving a lot of attention. Thus, this study was designed to assess the Eichhornia crassipes biochar amended pollutants sorption competence of multi-metal tolerant Aspergillus flavus on South Pennar River. The physicochemical characteristics declared that the, half of the parameters (turbidity, TDS, BOD, COD, Ca, Mg, Fe, free NH3, Cl-, and F-) of South Pennar River were beyond the permissible limits. Furthermore, the lab-scale bioremediation investigation with different treatment groups (group I, II, and III) revealed that the group III (E. crassipes biochar and A. flavus mycelial biomass) showed considerable remediation efficiency on South Pennar River water in 10 days of treatment. The metals adsorbed on the surface of E. crassipes biochar and A. flavus mycelial biomass was also affirmed by SEM analysis. Hence such findings, E. crassipes biochar amended A. flavus mycelial biomass could be a sustainable method of remediating contaminated South Pennar River water.

Simultaneous quantification of losartan potassium and its active metabolite, EXP3174, in rabbit plasma by validated HPLC-PDA.

Herein, we report a novel, accurate and cost-effective validated analytical method for the quantification of losartan potassium and its active metabolite, EXP 3174, in rabbit plasma by reversed-phase high-performance liquid chromatography. Valsartan was used as an internal standard. The method was validated as per International Conference on Harmonization guidelines. The analytes were extracted in rabbit plasma using liquid-liquid extraction technique and analyzed at 247 nm after separation through a reverse-phase C18 column. The isocratic mobile phase used is a mixture of acetonitrile, water and glacial acetic acid in the ratio of 60:40:1 v/v/v maintained at pH 3.4. All calibration curves showed a good linear relationship (r > 0.995) within the test range. Precision was evaluated by intra- and interday tests with RSDs <1.91% and accuracy showed validated recoveries of 86.20-101.11%. Based on our results, the developed method features good quantification parameters and can serve as an effective quality control method for the standardization of drugs.

Utilization of agricultural, industrial waste and nanosilica as replacement for cementitious material and natural aggregates - Mechanical, microstructural and durability characteristics assessment.

This study examines the effect of rice husk ash (RHA) and nanosilica, and ground granular blast furnace slag (GGBS) on concrete mechanical and durability properties. The cement had been partially replaced with nanosilica and RHA having substitution percentages up to 6% and 10% respectively whereas the sand had been partially replaced by GGBS at 20% for all mixes. A water-to-cementitious materials ratio of 0.38 and a sand-to-cementitious materials ratio of 2.04 were used to cast eight different concrete mixes. The nanosilica used in the present research possessed some favorable effects such as rich fineness, higher surface area and greater reactivity which signified one of the best cement replacement materials. Both the durability and strength of concrete specimens possessing nanosilica, RHA and GGBS was evaluated using in-elastic neutron scattering, SEM image, piezoresistive test, split tensile strength, flexural strength and compressive strength test. Concrete specimens were also subjected to chloride penetration and water absorption to examine the impact of replacement materials on the concrete's durability attributes. Concrete performance was increased by the ternary blending of concrete because of the active participation of nanosilica in durability and strength at early ages, both RHA and GGBS played an important role in improving packing density. It was found that as the percentage of cement replaced with nanosilica increases, the durability of concrete also significantly increases. But the optimum strength parameter was found when 4% of cement was replaced by the nanosilica effectively. The proposed ternary mix may be eco-friendly by saving cement and enhancing strength and durability effectively.

Comparison of pennywort and hyacinth in the development of membraned sediment plant microbial fuel cell for waste treatment.

Membraned Sediment Plant Microbial Fuel cells (SPMFCs) are appealing bioelectrochemical systems that generate power from organic compounds in sediment through exoelectrogen decomposition and are used to treat wastewater. This research was designed to develop a single-chambered sediment plant microbial fuel cell using two membrane electrodes; one carbon plate cathode and one anode. Wastewater and sediment mixture was sampled from Rawalpindi, Pakistan, and bacterial isolation was performed by serial dilution. Five strains were selected on the basis of morphology and growth-promoting characteristics. The selected strains were identified by 16s rRNA sequencing and designated as A (Geobacter sulfurreducens OP527025), B (Shawanella putrefaciens OP522353), C (Bacillus subtilus OP522349), D (Azospirillum humicireducens OP527050) and E (Pseudomonas putida OP526951). Consortium of five strains was developed. Two aquatic plants pennyworts (Hydrocotyle umbellate), and Hyacinth (Eichhornia crassipes) were used in the SPMFCs along with consortium. A maximum voltage of 1120mv was observed in SPMFCs treated with the consortium and water hyacinth, which was followed by 543.3 mv of SPMFCs treated with water pennyworts. Physicochemical analysis of wastewater showed a remarkable reduction of 74.5%, 71%, and 76% in nitrate, phosphate, and sulphate content of wastewater treated with microbes and water hyacinth. The heavy metal analysis showed a reduction of Zn (99.8%), Mg (99.9%), and Ni (98.4%) in SPMFCs treated with the consortium and water hyacinth. Mebraned SPMFCs showed an increase of 30% and 20% in shoot and root length of water hyacinth. A remarkable increase of 25%, 18%, and 12% were recorded in chlorophyll content, membrane stability index and relative water content of water hyacinth in SPMFCs treated with consortium compared to untreated cells. Osmolyte content had shown significant increase of 25% with consortium treated water hyacinth plant as compared to untreated one. An increase of 15%, 20% and 12% was noted in superoxide dismutase (SOD), peroxidase dismutase (POD) and catalase content of consortium treated water hyacinth as compared to control one. The present research gave insight into the potential of sediment plant microbial fuel cells along with aquatic plants for treatment of wastewater. This could be a effective method for removal of hazrdaous substances from wastewater and alternative approach for voltage production.

Chronic ozone exposure impairs the mineral nutrition of date palm (Phoenix dactylifera) seedlings.

Chronic ozone (O3) exposure in the atmosphere preferentially disturbs metabolic processes in the roots rather than the shoot as a consequence of reduced photosynthesis and carbohydrate allocation from the leaves to the roots. The aim of the present study was to elucidate if mineral nutrition is also impaired by chronic O3 exposure. For this purpose, date palm (Phoenix dactylifera) plants were fumigated with ambient, 1.5 × ambient and 2 × ambient O3 in a free air controlled exposure (FACE) system for one growing season and concentrations of major nutrients were analyzed in leaves and roots. In addition, concentrations of C and N and their partitioning between different metabolic C and N pools were determined in both organs. The results showed that calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), sodium (Na) and potassium (K) acquisition by roots was diminished by O3 exposure of the shoot. For Ca, Mg, Fe and Zn reduced uptake by the roots was combined with reduced allocation to the shoot, resulting in a decline of foliar concentrations; for Na and K, allocation to the shoot was maintained at the expense of the roots. Thus, elevated O3 impaired both mineral uptake by the roots and partitioning of minerals between roots and shoots, but in an element specific way. Thereby, elevated O3 affected roots and shoots differently already after one growing season. However, considerable changes in total C and N concentrations and their partitioning between different metabolic pools upon chronic O3 exposure were not observed in either leaves or roots, except for reduced foliar lignin concentrations at 2 × ambient O3. Significant differences in these parameters were shown between leaves and roots independent of O3 application. The physiological consequences of the effects of chronic O3 exposure on mineral acquisition and partitioning between leaves and roots are discussed.