Surfactants Influencing the Biocatalytic Performance of Natural Alkane-Degrading Bacteria via Interfacial Biocatalysis in Pickering Emulsions.

Setting superhydrophobic Mycobacterium sp. as an example, the hydrophobic bacteria acting as demulsifying agents of surfactant-stabilized conventional emulsions, vice versa, the synergistic/antagonistic influence of nonionic surfactants (Tween 80 or Span 80) on the stability of the bacteria-stabilized Pickering emulsions was investigated. At the same time, the activated/suppression effect of nonionic surfactants on microbial degradation of tetradecane, which exhibited a dose-response relationship, was also found. The hydrophobic bacteria acting as demulsifying agents and the suppression influence of nonionic surfactants on the biocatalytic performance (indexing as biomass) of natural alkane-degrading bacteria, believed to be totally separated concepts previously, are for the first time found to be closely related to in situ surface modification of bacteria with nonionic surfactants. During the degradation of tetradecane by Mycobacterium sp. in the presence of nonionic surfactants, demulsification due to the bacteria acting as demulsifying agents and interfacial biocatalysis in the bacteria-stabilized Pickering emulsions are involved, which provides useful information to select optimal dispersants for marine oil spills.

Study on the Quality, Relevance, and Comparability of YouTube Videos Expressing Stroke and Its Consequences From Various Sources.

Background and objective Stroke-related deaths have been one of the major causes of death worldwide due to its rising risk factors. As a result, several people rely on YouTube as a great source of information on stroke without knowing the genuineness of the content. This study aims to assess the quality and reliability of the information on stroke uploaded on the YouTube platform using the Global Quality score (GQS) and DISCERN score (DS), respectively. Methodology A cross-sectional observational study was conducted in April 2023. Stroke-related keywords were used to search for videos on YouTube. Videos that met inclusion criteria were evaluated for baseline characteristics (likes, comments, views, duration of video, time since posted, and uploader type) and type of information in the video about stroke (symptoms, etiology, treatment, and other parameters). These videos were then evaluated for quality and reliability of information using GQS and DS, respectively. Results After applying inclusion and exclusion criteria and removing the duplicates, 73 YouTube videos were selected. The videos had a total number of 23,927,445 views, 385,324 likes, and 31,927 comments. Maximum videos were uploaded by hospitals (25, 34.2%). Several videos described the symptoms (54, 73.97%), treatment (50, 68.49%), and etiology (49, 67.12%) of stroke. The reach of videos measured by the Video Power Index (VPI) was highest for videos uploaded by healthcare organizations (mean VPI = 120.11). There was no statistically significant difference (P > 0.05) in the quality (GQS score) and reliability (DS) of videos uploaded by doctors, hospitals, healthcare organizations, and news channels.  Conclusions YouTube can become an important source to disseminate information about health-related conditions like stroke if the videos uploaded are of high quality (GQS score) and reliable (DS).

Hierarchical SAPO-34 Catalysts as Host for Cu Active Sites.

Cu-containing hierarchical SAPO-34 catalysts were synthesized by the bottom-up method using different mesoporogen templates: CTAB encapsulated within ordered mesoporous silica nanoparticles (MSNs) and sucrose. A high fraction of the Cu centers exchanged in the hierarchical SAPO-34 architecture with high mesopore surface area and volume was achieved when CTAB was embedded within ordered mesoporous silica nanoparticles. Physicochemical characterization was performed by using structural and spectroscopic techniques to elucidate the properties of hierarchical SAPO-34 before and after Cu introduction. The speciation of the Cu sites, investigated by DR UV-Vis, and the results of the catalytic tests indicated that the synergy between the textural properties of the hierarchical SAPO-34 framework, the high Cu loading, and the coordination and localization of the Cu sites in the hierarchical architecture is the key point to obtaining good preliminary results in the NO selective catalytic reduction with hydrocarbons (HC-SCR).

Adding nanoparticles to improve emulsion efficiency and enhance microbial degradation in Pickering emulsions.

Interfacial microbial degradation of alkane in Pickering emulsions stabilized by hydrophobic bacterial cells is a new mechanism for microbial degradation of water-insoluble chemicals, where both water-insoluble chemicals in the oil phase and water-soluble nutrients (such as nitrogen and phosphorus) in the water phase are bio-accessible to living microorganisms anchoring onto the oil-water interfaces. In the present work, super-hydrophobic Mycobacterium sp. (contact angle 168.6°) degradation of tetradecane was set up as a model. Addition of fumed SiO2 particles (Aerosil® R974) as a new strategy was developed to enhance tetradecane degradation where the biodegradation rate (based on the accumulated biomass) increased by approximately 80%. The enhanced effect of SiO2 particles on the tetradecane degradation attributed to the synergistic effect of SiO2 particles on the emulsion efficiency of Pickering emulsions stabilized by bacterial cells and then on the enhancement of interfacial microbial degradation in Pickering emulsions. KEY POINTS: • Interfacial microbial degradation in bacterial cells stabilized Pickering emulsions. • Adding fumed SiO2 particles to enhance microbial degradation of tetradecane. • Correlation relationship between emulsion efficiency and interfacial microbial degradation.

Achieving the IA2030 Coverage and Equity Goals through a Renewed Focus on Urban Immunization.

The 2021 WHO and UNICEF Estimates of National Immunization Coverage (WUENIC) reported approximately 25 million under-vaccinated children in 2021, out of which 18 million were zero-dose children who did not receive even the first dose of a diphtheria-tetanus-pertussis-(DPT) containing vaccine. The number of zero-dose children increased by six million between 2019, the pre-pandemic year, and 2021. A total of 20 countries with the highest number of zero-dose children and home to over 75% of these children in 2021 were prioritized for this review. Several of these countries have substantial urbanization with accompanying challenges. This review paper summarizes routine immunization backsliding following the COVID-19 pandemic and predictors of coverage and identifies pro-equity strategies in urban and peri-urban settings through a systematic search of the published literature. Two databases, PubMed and Web of Science, were exhaustively searched using search terms and synonyms, resulting in 608 identified peer-reviewed papers. Based on the inclusion criteria, 15 papers were included in the final review. The inclusion criteria included papers published between March 2020 and January 2023 and references to urban settings and COVID-19 in the papers. Several studies clearly documented a backsliding of coverage in urban and peri-urban settings, with some predictors or challenges to optimum coverage as well as some pro-equity strategies deployed or recommended in these studies. This emphasizes the need to focus on context-specific routine immunization catch-up and recovery strategies to suit the peculiarities of urban areas to get countries back on track toward achieving the targets of the IA2030. While more evidence is needed around the impact of the pandemic in urban areas, utilizing tools and platforms created to support advancing the equity agenda is pivotal. We posit that a renewed focus on urban immunization is critical if we are to achieve the IA2030 targets.

Multivariate assessment of vaccine equity in Nigeria: A VERSE tool case study using demographic and health survey 2018.

Nigeria experiences wide heterogeneity in vaccination rates by vaccine and region. However, inequities in vaccination status extend beyond just geographic covariates. Traditionally, inequity is represented by a single metric pertaining to socioeconomic status. A growing body of literature suggests that this view is limiting, and a multi-factor approach is necessary to comprehensively evaluate relative disadvantage between individuals. The Vaccine Economics Research for Sustainability and Equity (VERSE) tool produces a composite equity metric, which accounts for multiple factors influencing inequity in vaccination coverage. We apply the VERSE tool to Nigeria's 2018 Demographic and Health Survey (DHS) to cross-sectionally evaluate equity in vaccination status for national immunization program (NIP) vaccines over the following contributing covariates: age of child, sex of child, maternal education level, socioeconomic status, health insurance status, state of residence, and urban or rural designation. We also assess equity for zero-dose, fully immunized for age, and completion of NIP. Results show that socioeconomic status contributes substantially to variation vaccination coverage, but it is not the most substantial factor. For all vaccination statuses, except for NIP completion, maternal education level is the greatest contributor towards a child's immunization status among model variables. We highlight the outputs for zero-dose, fully immunized at infancy, MCV1 and PENTA1. The percentage point gap in vaccination status between the top and bottom quintiles of disadvantage, as ranked by the composite indicator is 31.1 (29.5-32.7) for zero-dose status, 53.1 (51.3-54.9) for full immunization status, 48.9 (46.9-50.9) for MCV1, and 67.6 (66.0-69.2) for PENTA1. Though concentration indices indicate inequity for all statuses, full immunization coverage is very low at 31.5% suggesting significant gaps in reaching children after initial doses for routine immunizations. Applying the VERSE tool to future Nigeria DHS surveys can allow decisionmakers to track changes in vaccination coverage equity, in a standardized manner, over time.

Pinpointing basic sites formed upon incorporation of iron in hierarchical SAPO-11 using catalytic model reactions.

By utilizing previously established catalytic model reactions, a method for probing the topological location of transition metal sites incorporated in hierarchical silicoaluminophosphates (SAPOs) is presented. For the first time, iron(III)-incorporated hierarchical SAPO-11 (FeCTAB-11) was prepared and thoroughly characterized with conventional iron(III)-incorporated SAPO-11 (FeSAPO-11) as a reference. Initially, inductively coupled plasma - mass spectrometry (ICP-MS) indicated that the FeSAPOs contained similar amounts of metal (∼2.0 wt%), while N2-physisorption confirmed the bimodal porosity of the hierarchical FeCTAB-11. Furthermore, X-ray absorption spectroscopy (XAS) revealed that iron(III) was isomorphously incorporated into both SAPO-11 samples, whereas CO2-temperature programmed desorption (TPD) revealed the first reported presence of strong basic sites in the vicinity of a transition metal incorporated into a SAPO framework. The location of the basic sites, and thus the incorporated iron, was subsequently probed by studying the products of the base-catalyzed vapor phase isomerization of cyclohexanone oxime (Beckmann rearrangement, BMR) model reaction. Through an increased lifetime for the base-catalyzed production of aniline, the incorporated iron for FeCTAB-11 was found to be located in highly accessible mesopores, whereas the conventional FeSAPO-11 had incorporated iron located in its micropores. Lastly, the methanol-to-hydrocarbons (MTH) model reaction showed that both FeSAPOs only had Brønsted acid sites in the micropores of the structures. This was used to verify the pore connectivity of the hierarchical FeCTAB-11 by utilizing the base-catalyzed BMR mechanism's dependency on acid sites.

Combining cluster surveys to estimate vaccination coverage: Experiences from Nigeria's multiple indicator cluster survey / national immunization coverage survey (MICS/NICS), 2016-17.

In 2015 immunization stakeholders in Nigeria were proceeding with plans that would have fielded two nationally representative surveys to estimate vaccination coverage at the same time. Rather than duplicate efforts and generate either conflicting or redundant results, the stakeholders collaborated to conduct a combined Multiple Indicator Cluster Survey (MICS) / National Immunization Coverage Survey (NICS) with MICS focusing on core sampling clusters and NICS adding supplementary clusters in 20 states, to improve precision of outcomes there. This paper describes the organizational and technical aspects of that collaboration, including details on design of the sample supplement and analysis of the pooled dataset. While complicated, the collaboration was successful; it yielded a unified set of relevant coverage estimates and fostered some novel sub-national results dissemination work.

ß-Adrenergic receptor, an essential target in cardiovascular diseases.

ß-Adrenergic receptors (ßARs) belong to a large family of cell surface receptors known as G protein-coupled receptors (GPCRs). They are coupled to Gs protein (Gαs) for the activation of adenylyl cyclase (AC) yielding cyclic AMP (CAMP), and this provides valuable responses, which can affect the cardiac function such as injury. The binding of an agonist to ßAR enhances conformation changes that lead to the Gαs subtype of heterotrimeric G protein which is the AC stimulatory G protein for activation of CAMP in the cells. However, cardiovascular diseases (CVD) have been reported as having an increased rate of death and ß1AR, and ß2AR are a promising tool that improves the regulatory function in the cardiovascular system (CVS) via signaling. It increases the Gα level, which activates ßAR kinase (ßARK) that affects and enhances the progression of heart failure (HF) through the activation of cardiomyocyte ßARs. We also explained that an increase in GPCR kinases (GRKs) would practically improve the HF pathogenesis and this occurs via the desensitization of ßARs, which causes the loss of contractile reserve. The consistency or overstimulation of catecholamines contributes to CVD such as stroke, HF, and cardiac hypertrophy. When there is a decrease in catecholamine responsiveness, it causes aging in old people because the reduction of ßAR sensitivity and density in the myocardium enhances downregulation of ßARs to AC in the human heart.

Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China.

Based on the feature of high-altitude permafrost topography and the diverse microbial ecological communities of the Qinghai-Tibetan Plateau, soil samples from thirteen different collection points around Qinghai lake were collected to screen for extremophilic strains with the ability to degrade phenol, and one bacterial strain recorded as TIBETAN4 that showed effective biodegradation of phenol was isolated and identified. TIBETAN4 was closely related to Kocuria based on its observed morphological, molecular and biochemical characteristics. TIBETAN4 grew well in the LB medium at pH 7-9 and 0-4% NaCl showing alkalophilicity and halophilism. The isolate could also tolerate up to 12.5 mM phenol and could degrade 5 mM phenol within 3 days. It maintained a high phenol degradation rate at pH 7-9 and 0-3% NaCl in MSM with 5 mM phenol added as the sole carbon source. Moreover, TIBETAN4 could maintain efficient phenol degradation activity in MSM supplemented with both phenol and glucose and complex water environments, including co-culture Penicillium strains or selection of non-sterilized natural lake water as a culture. It was found that TIBETAN4 showed enzymatic activity of phenol hydroxylase and catechol 1,2-dioxygenase after induction by phenol and the corresponding genes of the two enzymes were detected in the genome of the isolate, while catechol 2,3-dioxygenase or its gene was not, which means there could be a degradation pathway of phenol through the ortho-pathway. The Q-PCR results showed that the transcripts of both the phenol hydroxylase gene and catechol 1,2-dioxygenase gene were up-regulated under the stimulation of phenol, demonstrating again that the strain degraded phenol via ortho-degradation pathway.

Strengthening Routine Immunization in Areas of Northern Nigeria at High Risk for Polio Transmission During 2012-2014.

BACKGROUND: Following the 2012 declaration by World Health Organization (WHO) Regional Director for Africa and the WHO Executive Board to ramp up routine immunization (RI) activities, began to intensify activities to strengthen RI. This study assessed how the intensification of RI helped strengthen service delivery in local government areas (LGAs) of northern Nigeria at high risk for polio transmission. METHODS: A retrospective study was performed by analyzing RI administrative data and findings from supportive supervisory visits in 107 high-risk LGAs. RESULTS: Our study revealed that administrative coverage with 3rd dose of diphtheria-pertussis-tetanus vaccine in the 107 high-risk LGAs improved from a maximum average coverage of 33% during the preintensification period of 2009-2011 to 74% during the postintensification period of 2012-2014. CONCLUSIONS: Routine immunization could be strengthened in areas where coverage is low, and RI has been identified to be weak when certain key routine activities are intensified.

Limitations of using administratively reported immunization data for monitoring routine immunization system performance in Nigeria.

BACKGROUND: Efforts are underway to strengthen Nigeria's routine immunization system, yet measuring impact poses a challenge. We document limitations in using administrative data from 12 states in Nigeria and explore alternative approaches. METHODS: We compared state-reported coverage with the third dose of diphtheria-tetanus-pertussis vaccine (DTP3) to district-reported coverage and data from coverage surveys conducted during 2006-2013. We used district-reported data during 2010-2013 to calculate the annual change in immunization coverage, the percentage of the target population that was unimmunized, and the number of vaccine doses administered. Data quality indicators were also assessed. RESULTS: State-reported DTP3 coverage was 66%-102% in 2010, 49%-98% in 2011, 38%-84% in 2012, and 75%-123% in 2013 and was a median 46%-114% greater than survey coverage during 2006-2013. The mean local government area (LGA)-reported coverage varied substantially (standard deviation range, 10%-33% across years). For 2010-2013, the mean annual percentage change in LGA-reported DTP3 coverage was -15% from 2010 to 2011, -9% from 2011 to 2012, and 74% from 2012 to 2013; the mean annual percentage change in the percentage of the target population unimmunized was -62%, 426%, and -62%, respectively; and the mean annual percentage change in the number of doses administered was -13%, -7%, and 90%, respectively. Annually, a mean 14% of LGAs reported DTP3 coverage of >100%. DISCUSSION: Assessing immunization system performance by using administrative data has notable limitations. In addition to long-term improvements in administrative data management, alternatives for measuring routine immunization performance should be considered.