Effects of genotype and environment on forage yield, nutritive value and morphology of lablab (Lablab purpureus (L.) sweet).

The goal of the study is to determine the effect of genotype and environment on forage yield, forage nutritive value and to determine the relation between morphology and forage yield and nutritive value of lablab. Thirteen genotypes (one local and 12 improved) were replicated 3 times in a randomized complete block trial across three locations in Ethiopian lowlands namely, Bechi, Kite and Tepi. All forage samples were analyzed for dry matter (DM), crude protein (CP), and in vitro dry matter digestibility (IVDMD) using a combination of conventional nutritional analyses and near infrared reflectance spectroscopy. There was a significant (P < 0.001) effect of genotype, location and genotype*location on forage yield of DM, forage yield of CP, forage yield of IVDMD, CP, and IVDMD. The difference between means of minimum and maximum genotypes was 12.9 t/ha of DM, 3.12 t/ha CP, 8.22 t/ha IVDMD, 57 g/kg of CP and 56 g/kg of IVDMD. The correlation between plant morphology and forage yield and nutritive value was weak (r ≤ 0.41) in all locations and the combined data. Both genotype and location should be considered by the farmers when they decide to grow lablab for forage production. Morphological traits of lablab are not suitable to evaluate forage yield and nutritive value. Enhancing the awareness of farmers about the effect genetic-environment interaction effect of forage yield and nutritive value and the relation between morphology and yield and nutritive value would improve the uptake of lablab in mixed the farming system leading to more sustainable agricultural production.

Effect of rosemary (Rosmarinus officinalis) leaf meal supplementation on production performance and egg quality of laying hens.

This experiment was conducted to investigate the supplemental effect of rosemary leaf meal (RLM) on egg production and egg quality in Lohmann-Brown laying hens. For each treatment, six replicates were performed with four 24-week-old laying hens for a total of 96 chickens. Dried rosemary leaf meal (RLM) was present in the commercial laying hen ration at 0, 1.7, 3.5 and 5.2%, respectively. The interactions between dietary treatments, egg storage temperatures (4 °C, 21.5 °C), and egg storage durations (7, 14, 21, 28, and 35 days) were examined in factorial designs using a fully randomized design. The data were analyzed using the Statistical Analysis System (SAS, 2014, Ver. 9.3) and Tukey's multiple range tests to separate the means. The inclusion of dried RLM at a level of 5.2% (p < 0.05) significantly improved the hens' daily egg production (78.9%). Feed conversion ratio (2.25) and egg weight (56.7 g) compared to control treatment. Similarly, at 3.5% and 5.2% RLM supplementation, eggshell weight (5.876 g) and eggshell strength increased significantly (p < 0.05) (compared to control, a weight of 3.76 kg/cm2 and a shell thickness (0.37 mm) was observed. In addition, the groups fed 5.2% RLM had significantly higher albumin weight (34.9 g/L), albumin height (6.9 mm), and yolk weight (15.6 g/L). l), yolk height (15.9 mm), yolk color (5.3 points), and Hough unit (83.9 points) than the control groups. Regarding the external characteristics of stored eggs, a group of hens fed at rates of 3.5 and 5.2% RLM showed significantly greater (p < 0.05) egg weight with increases in storage temperature and duration as a control group. There was a significant interaction effect between stored eggs collected from the supplemented groups in terms of egg weight, weight loss, shell weight, shell strength, and shell thickness stored at specific temperatures and storage durations. There was a decrease in albumen level and Haugh unit (HU) with an increase in storage temperature and duration over treatment (P < 0.05). Therefore, better external quality was observed in eggs collected from a group of chickens fed 4.5 and 5.2% RLM after 35 days of storage compared to a control group. Mean albumen height, albumen weight, HU, yolk height, and yolk color of eggs stored in the refrigerator (4 °C) were an exception for yolk weight, which was higher compared to room temperature (21.5 °C). Significantly low (P < 0.05) albumen height (6.61 to 2.96 cm), Hough unit (82.49 to 47.64 points), and yolk height (14.66 to 12.35 mm) were observed at 35 days storage recorded in the control group. In conclusion, supplementation with RLM at 3.5% and 5.2% improved the performance and quality of both fresh and stored eggs.

Spineless cactus cladode is a viable replacement to barley and maize grains in the feed rations of dromedary camel calves.

BACKGROUND: No studies determined the use of spineless cactus cladodes in camel diets. OBJECTIVES: The effect of replacing the main energy source in camel diets with fresh spineless cactus cladodes on growth performance was determined. Furthermore, the ability of morphology to predict cladodes yield was determined. METHODS: A prediction model of spineless cactus cladode weight based on cladode volume was developed. Three cladodes per plants were randomly selected from 100 plants. Weight and volume were then recorded for each cladode. Sixteen male camel calves (196 ± 18.2 kg live weight and 430 ± 5.55 days of age) were allotted to treatments, control (conventional camel fattening diet based on cotton seed hulls, cereal grains and agro-industrial by-products) or cactus (barley and maize grains in the control diet were replaced totally by fresh cactus cladode on dry matter basis). The study contained a 100-days growth trial and a 21-day digestibility trial (15 days of adaptation and 7 days of faeces collection). Blood samples were collected monthly from each animal. RESULTS: Cactus cladode volume predicted the dry weight with a high accuracy (prediction error = 3.5%). Nutrient intake and nutrient digestibility did not significantly differ among the dietary treatments (p > 0.05). The treatment significantly decreased feed conversion ratio by 1.52 points (p < 0.05). All blood parameters were within the normal range of dromedary camels. CONCLUSIONS: Spineless cactus cladode is a potential replacement to the conventional energy sources in dromedary camel diets.

Can the broiler industry rely on results of existing life cycle assessment and environmental assessments studies to inform broilers' nutritional strategies?

The goal of this systematic review is to investigate the applicability of the results from existing life cycle analysis (LCA) and environmental assessments studies in informing nutritional strategies for environmentally sustainable poultry meat production. This paper reports on a Rapid Evidence Assessment (REA) of articles published between 2000 and 2020. The studies reviewed were conducted in developed countries including UK, France, Germany, Sweden, Norway, The Netherlands, Denmark, Belgium, Canada, and USA. All articles were written in English. The REA includes studies on LCA of differing strains of meat poultry and production systems, studies on poultry manure emission and studies on environmental assessments of plant-based feed ingredients. The review covered studies on soil carbon dynamics associated with plant-based ingredients. Web of Science, Scopus, and PubMed were used to obtain the 6,142 population articles. The multistage screening process resulted in 29 studies from which 15 studies included LCA while the rest 14 studies analyzed NH3 emission of broilers. All studies based on LCA were descriptive and did not include replications. Only 12 studies assessed the effect of interventions to reduce NH3 emission of broiler litter using replicated layout designs. It is concluded that the broiler industry in UK, EU, and North America cannot rely on results of existing LCA and environmental assessments studies to inform their nutritional strategy and poultry meat production due to a shortage of reliable in vivo data assessing interventions in controlled studies.

Corncob biochar supplementation improves nutrient digestibility, fattening performance and carcass characteristics of fattening sheep.

BACKGROUND: Corncob is a cheap source for biochar in developing countries. No studies reported on use of biochar as sheep fattening supplement. OBJECTIVES: The goal of this study is to determine the effect of corncob biochar supplementation on growth performance and carcass characteristics of sheep. METHODS: Twenty-four Horro yearling rams (18.6 ± 0.938 kg live weight) were randomly distributed to four groups, control (300 g/day concentrate + local hay ad libitum), BC1.5 (control +1.5 g/day biochar), BC3 (control +3 g/day biochar) and BC4.5 (control +4.5/day biochar). The experimental concentrate consisted of 200 g/day wheat bran and 100 g/day Noug cake. The study consisted of a growth trial of 111 days followed by a digestibility trial of 10 days. Carcass characteristics for all rams were recorded at the end of the trial. RESULTS: Dry matter intake, crude protein intake, dry matter digestibility and acid detergent fibre digestibility of BC1.5 were significantly higher than the control. Corncob biochar supplementation significantly improved daily weight gain and feed conversion ratio of the rams. Supplementation sheep with corncob biochar improved significantly dressing percentage, hindquarters weight, forequarters weight, ribs weight, brisket weigh and rib eye muscle area. There was a significant effect of the dietary treatment on weight of internal organs; however, they were all within the normal range of sheep. CONCLUSIONS: Corncob biochar supplementation would be recommended at a level of 1.5 g/day to improve growth performance and carcass characteristics of sheep.

Optimizing near Infrared Reflectance Spectroscopy to Predict Nutritional Quality of Chickpea Straw for Livestock Feeding.

Multidimensional improvement programs of chickpea require screening of a large number of genotypes for straw nutritive value. The ability of near infrared reflectance spectroscopy (NIRS) to determine the nutritive value of chickpea straw was identified in the current study. A total of 480 samples of chickpea straw representing a nation-wide range of environments and genotypic diversity (40 genotypes) were scanned at a spectral range of 1108 to 2492 nm. The samples were reduced to 190 representative samples based on the spectral data then divided into a calibration set (160 samples) and a cross-validation set (30 samples). All 190 samples were analysed for dry matter, ash, crude protein, neutral detergent fibre, acid detergent fibre, acid detergent lignin, Zn, Mn, Ca, Mg, Fe, P, and in vitro gas production metabolizable energy using conventional methods. Multiple regression analysis was used to build the prediction equations. The prediction equation generated by the study accurately predicted the nutritive value of chickpea straw (R2 of cross validation > 0.68; standard error of prediction < 1%). Breeding programs targeting improving food-feed traits of chickpea could use NIRS as a fast, cheap, and reliable tool to screen genotypes for straw nutritional quality.

Effect of Barley Variety on Feed Intake, Digestibility, Body Weight Gain and Carcass Characteristics in Fattening Lambs.

Twenty lambs (18 ± 0.22 kg initial weight) were blocked by weight and individually assigned into pens to evaluate the effects of barley straw variety on digestibility, growth performance and carcass characteristics. The following four treatments were tested: (1) a local barley straw (as control), (2) HB1963 (high grain and straw yields), (3) Traveller (high straw yielder), and (4) IBON174/03 (high grain yielder). A concentrate (50:50 wheat bran and noug seed cake) was offered constantly (300 DM g), whereas the straw was offered ad libitum. The digestibility trial lasted 22 days (15 days to adapt to dietary treatments and 7 days for sampling). The growth performance trial lasted 90 days. At the end, all of the lambs were slaughtered, and their carcasses were evaluated. The IBON174/03 variety had a higher (p < 0.05) intake of organic matter and crude protein, a higher dry matter and organic matter digestibility than the control, and a faster growth than the control. The feed-to-gain ratio was similar among treatments. The slaughter and empty body weights of lambs in the IBON174/03 group were higher than the control variety (p < 0.05). The present study showed that the feeding value of barley straw can differ substantially between varieties and therefore must be considered in the choice of a barley variety.

Evaluation of dried papaya pomace meal in laying hen diets.

In the search for alternative feed resources for laying hens, papaya pomace is available as industrial by-product but information on its nutritive value is lacking. Dried papaya pomace was included in a common laying hen diet at 0%, 2.5%, 5% and 7.5% to evaluate its effect on egg production performance, egg quality and general health parameters in Bovan brown layers. For every inclusion level, three cages with ten 20-week-old layers were used, making a total of 120 hens. The effect of dried papaya pomace inclusion on egg production, egg quality and general health parameters was evaluated. Dried papaya pomace inclusion improved egg production and laying by 6.15% and 17% respectively, while it significantly decreased feed conversion ratio by 7.5%. Eggshell weight, thickness and strength of PP5 were higher than the control by 0.3 g, 0.8 mm and 0.43 kg/cm2 , respectively. There was a significant improvement in albumin weight (by 1.5 g/L), albumin height (2.1 mm), yolk weight (0.4 g/L), yolk height (0.4 mm), yolk colour (4.4 points) and Haugh unit (8 points) due to PP5 treatment. Inclusion of papaya pomace at a level of 7.5% of layers diet had negative effect on Egg production, feed conversion ratio and interior and exterior egg quality traits. Inclusion of papaya pomace affected significantly serum total cholesterol, serum triglyceride, serum low density lipoprotein, serum high density lipoprotein, aspartate aminotransferase, alanine aminotransferase and white blood cell count. However, all blood traits of the experimental animals were within the normal ranges reported for layers. Inclusion of papaya pomace in 5% of layers commercial diets improves egg production and quality without negative impact on health while decreasing feeding cost.

Feasibility of heart girth models in estimating live weight of fat-long-tailed sheep.

Fat deposition in the brisket of Ethiopian fat-long-tailed sheep may interfere with the correlation between heart girth (HG) and live weight (LW), bringing into question the accuracy of HG models for LW prediction that are currently in use. This study assessed the accuracy of published HG-based prediction models of the live weight of Ethiopian sheep breeds. Furthermore, the study identified accurate and robust models that predict the LW of the sheep using HG. Live weight and HG of 1,020 sheep from Bonga, Adilo and Horro breeds were measured. First, data collected from the study was used to gauge the preciseness of previously published prediction models of each breed. Second, the data of individual breeds were divided into a calibration set for model construction and a validation set for model validation. Live weight was regressed on HG to construct simple linear, Box-Cox, quadratic and allometric prediction models. Prediction error of published models was >20%. Models constructed for each breed did not differ in R2 . However, only simple linear models with transformed LW (Adilo: Log10 (LW) = 0.408 + 0.015*HG, Bonga: Log10 (LW) = -36.6 + 0.882*HG, Horro: LW0.5  = -1.26 + 0.085*HG) had homogenous residuals and prediction error of ≤ 10%. Heart girth models currently used to predict LW of Adilo, Bonga and Horro sheep of Ethiopia are not sufficiently accurate as they have PE higher than 10%. Prediction models generated by the current study could replace the published models for an accurate estimation of LW of the three breeds for husbandry, marketing and veterinary purposes.

Effect of olive tree leaves and twigs on intake, digestibility, growth performance and blood variables of Shami goats.

The effect of replacing wheat straw and a portion of commercial concentrate with olive twigs and leaves treated with urea and molasses on nutrient digestibility, growth performance and blood variables was determined. Forty-two Shami goat kids (20 ± 2.47 kg live weight and 133 ± 5.44 days of age) were randomly allotted to three dietary treatment groups, control (75% commercial concentrate and 25% wheat straw), OTU (62.3% commercial concentrate + 37.7% olive twigs and leaves treated with 4% urea for 14 days), OTUM (51.5% concentrate mixture + 48.5% olive twigs and leaves treated with 4% urea for 14 days then sprayed by 10% molasses). Cost (SYP/t) of control, OUT and OTUM was 12,600, 10,066 and 10,106 respectively. The study consisted of a growth trial of 100 days and a digestibility trial of 12 days (7 days of adaptation and 5 days of urine and faeces collection). Serum urea, total protein, albumin, alanine aminotransferase activity, aspartate aminotransferase activity, glucose, cholesterol, creatinine, haemoglobin and packed cell volume were determined. Daily dry matter intake, digestibility of crude protein, ether extract and nutrient detergent fibre did not significantly differ among the dietary treatments (p > .05). Nitrogen intake, nitrogen excretion in urine and faeces and nitrogen balance was not significantly affected (p > .05) by the dietary treatment. The dietary treatment did not have any significant effect on average daily gain, feed conversion ratio or blood variables (p > .05). Olive twigs treated with 4% urea and OTUM replaced 37.7% and 48.5% of conventional diets of growing Shami goat kids without negative effects on growth performance nor health and potentially reduced fattening costs for farmers in olive-livestock mixed systems by 19.8 and 20%, respectively. OUT is technically simpler than OTUM, thus, it could replace the conventional fattening ration of Shami male goat.

Bioavailability of Methionine-Coated Zinc Nanoparticles as a Dietary Supplement Leads to Improved Performance and Bone Strength in Broiler Chicken Production.

Recently, nanotechnology has been widely adopted in many fields. The goal of this study was to evaluate the potential for amino acid coated nano minerals as a supplement in broiler feed. Zinc was selected as a model mineral for this test and supplementation of nano zinc, both coated and uncoated was compared with organic and inorganic commercial forms of zinc. A total of 48 pens (8 birds each) were assigned to one of the following dietary treatments: Control, methionine-Zinc chelate (M-Zn), nano zinc oxide (Nano-ZnO), and methionine coated nano zinc oxide (M-Nano-ZnO). All experimental diets were formulated with the same total zinc, methionine, protein, and energy content with just the zinc source as a variable. Bird weight, feed intake and feed conversion ratios were recorded weekly, with three birds culled (sacrificed) at day 21 and day 35 for sampling measures. Ileal digestibility of zinc was determined at day 21 and day 35 using titanium dioxide as an inert marker. Blood serum, liver and spleen samples were collected at day 21 and day 35 and analysed for zinc content via inductively coupled plasma mass spectrometry (ICP-MS). Tibia strength and morphometrics were measured from both legs of three birds per pen at day 21 and day 35. The study was conducted at Nottingham Trent University Poultry Unit, UK. The novel method of producing nano minerals coated with amino acids was successfully tested with zinc and material produced to test in the feeding study. Methionine coated nano zinc oxide supplementation significantly improved bird weight gain and the increased feed intake of broilers compared to an inorganic zinc form. Ileal digestibility was also improved with this methionine-nano zinc. Moreover, this supplementation improved the tibia strength of broilers at the age of 21 days, though this was not observed at day 35. Therefore, M-Nano-ZnO could be used to supplement broilers to improve both performance and digestibility with a limited positive impact on bone strength. The results of the current study suggest that the amino acid coating of nano minerals can improve the digestibility of minerals which may have further implications for the field of mineral nutrition in animal feeds.

Spineless cactus (Opuntia ficus-indica) and saltbush (Atriplex halimus L.) as feed supplements for fattening Awassi male lambs: effect on digestibility, water consumption, blood metabolites, and growth performance.

The effect of replacing 13.6% and 20.3% of a total ration of fattening Awassi lambs by two combinations of fresh saltbush (Atriplex halimus) and fresh spineless cactus (Opuntia ficus-indica) cladodes at a ratio of 1.9:1 (TRT1) and 1.7:1 (TRT2) on water intake, digestibility, blood metabolites, and fattening performance was evaluated. Thirty-six lambs with average initial live weight 34.5 ± 4.18 kg were randomly assigned to three diets (control, TRT1, and TRT2). The control received a diet containing 166 g/kg barley straw and 834 g/kg of commercial concentrate mixture; TRT1 comprised 126 g barley straw, 739 g/kg concentrate mixture, 47 g/kg spineless cactus, and 89 g saltbush; TRT2 comprised 67 g/kg barley straw, 704 g/kg commercial concentrate mixture, 86 g/kg spineless cactus, and 144 g saltbush. A growth trial of 100 days (10 days of adaptation and 90 days of collection) followed by a metabolism trial of 17 days (10 days of adaptation and 7 days of a total feces and urine collection) was carried out. Daily dry matter intake, digestibility of crude protein, ether extract and nutrient detergent fiber, nitrogen balance, average daily gain, feed conversion ratio, and blood metabolites were not significantly affected by the treatment. Water consumption in TRT2 was significantly 16% less compared with the control. A combination of saltbush and spineless cactus at a ratio of 1.7:1 (TRT2) replaced 60% of barley straw and 16% of concentrate mixture without adverse effects on health and growth performance of Awassi male lambs. This represents a potential reduction in feed costs for smallholder farmers.

Integrating straw yield and quality into multi-dimensional improvement of lentil (Lens culinaris).

BACKGROUND: Lentil straw is an important source of fodder for livestock in Africa, South Asia and the Middle East. However, improvement programmes of lentil do not pay attention to straw traits, neither are straw traits considered in release criteria of new varieties. This study aimed to determine whether straw traits can be integrated into multi-trait improvement of lentil. RESULTS: Wide genotypic variation (P < 0.001) was found in grain yield, straw yield and nutritive value of straw. Urea treatment significantly (P < 0.01) improved the nutritive value of straw; however, the genotypic range was comparatively higher by 13.3 units, 56 units, 0.82 units, 106 units, 18.3 units and 1.62 units in crude protein, in vitro organic matter digestibility, metabolisable energy, potential dry matter intake, potential crude protein intake and potential metabolisable energy intake respectively. Acid detergent fibre correlated very strongly (pooled r = 0.87) with other nutritive value parameters of straw, therefore, it can be used to screen lentil varieties for fodder quality. Furthermore, acid detergent fibre can accurately predict in vitro organic matter digestibility (R2 = 0.9) and metabolisable energy (R2 = 0.8). Straw yield weakly correlated (r = 0.39, P < 0.001) with grain yield while no relation (P > 0.05) was found between grain yield. CONCLUSION: The possibility to simultaneously improve grain yield and nutritive traits of lentil straw. © 2017 Society of Chemical Industry.