RESUMEN
BACKGROUND: HIV continues to spread at high rates in sub-Saharan Africa. In particular, Swaziland is one of the countries most affected by the HIV/AIDS pandemic. Monitoring of HIV infection in Swaziland is being made by periodical investigations on HIV prevalence in pregnant women. However, knowledge of proportion of recent HIV infections is important for epidemiologic purposes to assess HIV transmission patterns. OBJECTIVES: To evaluate the proportion of recent HIV infections among pregnant women and its change overtime and to analyze factors associated with recent HIV infection in Swaziland. STUDY DESIGN: HIV-positive sera from pregnant women were collected during the 2004 and 2006 National HIV Serosurveys conducted in Swaziland and tested for the HIV antibody avidity, in order to identify recent HIV infections. Socio-demographic and clinical information was also collected. A multivariate analysis was conducted to assess the association between recent HIV infection and socio-demographic and clinical factors. RESULTS: A total of 1636 serum samples were tested for HIV antibody avidity. The overall proportion of recent infections was 13.8%, with no significant difference between 2004 and 2006 (14.6% vs. 13.1%, P>0.05, respectively). At the multivariate analysis, the younger age [14-19 vs. >or=20 years; adjusted odds ratio (aOR) 2.17, 95% CI: 1.45-3.24], as well as being at first pregnancy (1 vs. >or=2; aOR 1.61, 95% CI: 1.10-2.35) was independently associated with recent HIV infection. CONCLUSIONS: This study shows no significant difference in the proportion of recent infections between 2004 and 2006 and suggests that young women and women at their first pregnancy are currently high-risk groups for HIV acquisition, highlighting the importance of developing targeted youth programmes to reduce the spread of HIV infection in the country.
Asunto(s)
Infecciones por VIH/diagnóstico , Infecciones por VIH/epidemiología , Complicaciones Infecciosas del Embarazo/diagnóstico , Adolescente , Adulto , Factores de Edad , Afinidad de Anticuerpos , Esuatini/epidemiología , Femenino , Número de Embarazos , Anticuerpos Anti-VIH/sangre , Infecciones por VIH/patología , Infecciones por VIH/transmisión , Humanos , Persona de Mediana Edad , Embarazo , Prevalencia , Factores de Riesgo , Adulto JovenRESUMEN
Aim of this study was to assess HIV-1 subtype distribution and prevalence of transmitted mutations related to antiretroviral drugs in Swaziland. According to the WHO guidelines, 47 plasma samples from naive patients stored at HIV/AIDS National Reference Laboratory in Mbabane between 2002 and 2003, before the introduction of antiretroviral therapy in the country, were studied. HIV-1 RNA was extracted from the plasma samples, RT and protease regions of pol gene were amplified and sequenced. The mutations associated to drug resistance were defined as major or minor on the basis of the recommendations of the International AIDS Society-USA panel. A mutation associated to non nucleoside inhibitors (Y181I) was found in one case showing a prevalence of transmitted drug resistance <5% in Swaziland. No major mutations conferring resistance to protease and nucleoside RT inhibitors were found. Clade assignment was performed by phylogenetic analysis of pol gene. The general time-reversible model of substitution was used to study the phylogenetic relationships between sequences obtained from Swazi patients and sequences from neighbor countries. All patients were found to carry a C subtype. No phylogenetic relationships were detected within Swazi sequences, indicating the absence of epidemiological relationships among patients in study. Although local variants of subtype C have been recently recognized, phylogenetic analysis did not reveal the presence of significant cluster of Swaziland sequences within African variants. This finding may be explained by multiple introduction of C strains.