Bioinspired multicolour carbon dots: comprehensive cytotoxicity, phytotoxicity, and bioimaging in animal cells and plants.

During the past decades, carbon dots (CDs) as a kind of nanoparticles with interesting fluorescence properties have retained their place as one of the best bioimaging agents, although their effects on plants have been rarely studied. In this study, we synthesized two kinds of concentration-dependent multicolour CDs using two solvent approaches, phosphate-buffered saline (PBS) and ethanol 20%. We confirmed the nature of the CDs through Fourier transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, zeta potential, X-ray powder diffraction, and high-resolution transmission electron microscopy. Afterwards, the cytotoxicity, phytotoxicity, and bioimaging of animal cells and plants using both synthesized CDs were examined. Eventually, PBS-based CDs were recommended during this study as an efficient bioimaging agent for animal cells and plants because of the appealing features of this CD, such as a small size range of less than 10 nm, surface charge with an average of -24 mV, a high quantum yield of 35.82%, the higher fluorescence intensity of ~400 a.u. for blue fluorescence light and 250 a.u. for green fluorescence light. Other features showing the superiority of PBS-based CDs include high photostability, low phytotoxicity (p ≤ 0.05 and p ≤ 0.01) and above all, there was no significant cytotoxicity at the concentration range of 500-7.81 µg/ml.

New generation of viral nanoparticles for targeted drug delivery in cancer therapy.

Nanoscale engineering is one of the novel methods to cure multitudes of diseases, such as types of cancers, neurological disorders, and infectious illnesses. Viruses can play a vital role in nanoscale engineering due to their specific properties like minuscule size, high stability in different body conditions, and large-scale production. Viral-like particles (VLPs) as specific nanoscale scaffolds can encapsulate a wide range of cargos, including nucleic acids, proteins, peptides, and drugs. The Exterior portion of VLPs can be changed by genetical or chemical conjugation as well as targeting ligands or peptides. The aforementioned features of VLPs can be used in several applications, such as drug delivery, bioimaging, tissue engineering, vaccine production, and disease detection. This review article attempts to investigate appearance characteristics, modification strategies, and manufacturing methods of VLPs. Additionally, drug delivery to cancer cells as one of the VLPs applications along with different cellular uptake mechanisms of VLPs by cancer cells are chosen for investigation. This review also tries to gather most of the recent studies of drug delivery to cancer cells by VLPs.

Designing of a pH-activatable carbon dots as a luminescent nanoprobe for recognizing folate receptor-positive cancer cells.

During recent years, cancer has been recognized as a well-known disorder all over the world. One of the important factors to tackle this problem better than past decades is early diagnosis that takes into practice by state-of-the-art visual equipment for detection cancer cells. Herein, in this research, we synthesized carbon dots with pH-dependent behavior from a green source by hydrothermal method with high quantum yield and blue fluorescence. Folic acid-conjugated carbon dots by an efficient and optimal conjugation method were set upped which determined cancer cells visually. These synthesized and conjugated nanoparticles entered into the cancer cells more comprehensive than normal cells by receptor-mediated endocytosis and could distinguish cancer cells from normal ones by fluorescence imaging. Ultimately, synthesized nanoparticles in this research can be considered as an efficient fluorescent nanoprobe for cancer pre-diagnosis.