RESUMEN
Phytochemicals that have antioxidant effect play important role in protection against several diseases in humans. This study was carried out to evaluate the efficacy of hesperidin and tiger nut against the early changes that may be related to the toxicity of acrylamide in female rats. 72 Sprague Dawley female rats were divided into six groups (12 rat/group): control group (I); hesperidin (HES) treated group (II); tiger nut (TN) treated group (III); Acrylamide (ACR) treated group (IV); HES-ACR treated group (V); and TN-ACR treated group (VI). There was a significant increase in the levels of serum carcino embryonic antigen (CEA), malondialdehyde (MDA), protein carbonyls (CO), ALT, AST, LDH, urea and creatinine while no significant changes of serum total sialic acid, progesterone (prog) and estradiol (E2) levels, and significant decreases of body weights, catalase (Cat) activity, superoxide dismutase (SOD) activity, reduced glutathione (GSH) level, and glutathione peroxidase (GSH-Px) activity of ACR treated group compared with the control. Our results suggested that supplementation of a diet with hesperidin provided antioxidant defense more significant than tiger nut against the toxicity of ACR in breast, liver and kidney tissues.
Asunto(s)
Acrilamida/toxicidad , Antioxidantes/farmacología , Cyperus/química , Contaminantes Ambientales/toxicidad , Hesperidina/farmacología , Preparaciones de Plantas/farmacología , Animales , Femenino , Riñón/efectos de los fármacos , Pruebas de Función Renal , Hígado/efectos de los fármacos , Pruebas de Función Hepática , Tubérculos de la Planta/química , Polvos , Ratas Sprague-DawleyRESUMEN
Nutritional studies recommend the regular consumption of fruits and vegetables to favor a healthy quality of life. This study was carried out to evaluate the efficacy of hesperidin and tiger nut against the carcinogenic activity of DMBA in female rats. 72 adult Sprague Dawley female rats were divided equally into six groups: control group (I); Hesperidin treated group (II); Tiger Nut treated group (III); DMBA treated group (IV); HES-DMBA treated group (V); and TN-DMBA treated group (VI). There was a significant increase in serum levels of carcinoembryonic antigen, total sialic acid, progesterone, estradiol, ALT, AST, LDH, urea and creatinine, and significant decrease in reduced glutathione level, superoxide dismutase, catalase and glutathione peroxidase activities of DMBA treated group compared to control. In conclusion, our results suggested that supplementation of diets with hesperidin provided antioxidant and chemoprotective activities more significant than tiger nut against the toxicity of DMBA in breast, liver and kidney tissues.
Asunto(s)
Carcinogénesis/patología , Cyperus/química , Hesperidina/uso terapéutico , 9,10-Dimetil-1,2-benzantraceno , Alanina Transaminasa/sangre , Animales , Aspartato Aminotransferasas/sangre , Peso Corporal/efectos de los fármacos , Carcinogénesis/metabolismo , Catalasa/metabolismo , Estradiol/sangre , Femenino , Glutatión Peroxidasa/metabolismo , Hesperidina/farmacología , Riñón/efectos de los fármacos , Riñón/patología , Hígado/efectos de los fármacos , Hígado/patología , Malondialdehído/sangre , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/patología , Ácido N-Acetilneuramínico/sangre , Progesterona/sangre , Carbonilación Proteica/efectos de los fármacos , Ratas Sprague-Dawley , Superóxido Dismutasa/metabolismoRESUMEN
PURPOSE: The purpose of this study was to evaluate the efficacy of hesperidin (HES), a citrus flavonoid, against the severity of biochemical disorders in the cerebral hemispheres of irradiated rats. MATERIAL AND METHODS: Hesperidin (50 mg/kg body weight) was administered to male albino rats via gavages during 10 successive days before whole body exposure to gamma rays (5 Gy) and during 14 days after irradiation. The animals were sacrificed on the 14th day post-irradiation. RESULTS: The results demonstrated a significant increase of the levels of thiobarbituric acid reactive substances (TBARS), protein carbonyls (CO), and advanced oxidation protein products (AOPP), associated to significant decreases of total superoxide dismutase (tSOD) and catalase (CAT) activities, and reduced thiols content in the cerebral hemispheres of irradiated rats indicating oxidative stress. A significant decrease of the serotonin (5-HT), dopamine (DA), norepinephrine (NE) and epinephrine (EPI) contents and a significant increase of the activity of monoamine oxidase (MAO) were recorded, also, indicating alterations in the metabolism of monoamines. Moreover, a significant decrease of the activities of glutamate dehydrogenase (GDH) and creatine phophokinase (CPK), and a significant increase of calcium ions (Ca (+2)) levels were recorded in the mitochondria. Hesperidin treatment has significantly attenuated oxidative stress, monoamines alterations and mitochondrial damage in the cerebral hemispheres of irradiated rats. CONCLUSION: It could be concluded that hesperidin might attenuate the severity of radiation-induced biochemical disorders in brain tissues.
