Simultaneous occurrence of Epstein-Barr virus (EBV) in periodontal pockets and in oral squamous cell carcinoma: a cross-sectional study.

OBJECTIVES: This study aimed to investigate the detection of Epstein-Barr virus (EBV) in oral squamous cell carcinoma (OSCC) and to verify the concordance of EBV-DNA frequency in subgingival sites and in the OSCC. METHODS: A cross-sectional study with 30 OSCC patients, aged from 44 to 88 years old, was conducted. Samples were collected in subgingival sites and at the OSCC, then submitted to DNA isolation, qPCR, and genotyping. Descriptive statistic was performed to report the frequency of EBV-DNA in all samples, and McNemar test was applied to verify the concordance among the EBV-DNA frequency in both sites. RESULTS: The individuals presented 62 years old in average, and the majority were male (66.6%). EBV-DNA was detected in 56.7% OSCC lesions. Among the subgroup of 19 dentate individuals, high concordance (73.7%) in both EBV-DNA detection and the absence in subgingival sites and OSCC was observed, and it was statistically significant (p < 0.05). CONCLUSIONS: We report the notable occurrence of EBV-DNA in OSCC; also, the presence of EBV in periodontal sites may contribute to find it in OSCC, although the possible contribution of EBV in the OSCC remains to be investigated. CLINICAL RELEVANCE: The identification of this easily accessible site of EBV latent infection may help to improve the patient's quality of life by maintenance of oral/periodontal health condition and preventing further possible disorders related to the virus, and also encourages new approaches for investigating EBV, periodontitis, and OSCC.

Epstein-Barr virus (EBV) in periodontal sites of human immunodeficiency virus (HIV)-positive individuals in North Brazil: a cross-sectional study.

OBJECTIVE: This study aimed to investigate the Epstein-Barr virus (EBV) prevalence and viral load in subgingival sites of human immunodeficiency virus type 1 (HIV-1) positive (HIV+) individuals, correlating subgingival EBV load to the clinical periodontal condition, HIV systemic load, EBV systemic load, and use of antiretroviral therapy (ART). METHOD AND MATERIALS: Ninety individuals were recruited and divided into three categories: those without periodontal disease (G1), with gingivitis (G2), and with periodontitis (G3). Subgingival biofilm and blood samples were analyzed by quantitative polymerase chain reactions (qPCR). A questionnaire was administered to collect general information about patients, and data regarding HIV and use of ART were accessed from their medical records. RESULTS: EBV was detected in 85.6% of the samples. Comparing subgingival and systemic load of EBV in G1, G2, and G3, there was a statistical difference only in G3 (3.93 log10 copies/mL and 5.47 log10 copies/mL, respectively; P = .014), where EBV load was higher in periodontal pockets than in the blood. All groups had high EBV loads in subgingival sites (> 2,000 copies/mL). A positive linear correlation between systemic HIV load and EBV subgingival load was found in G1 and G2 (r = 0.647; P < .001), but not in G3. Only G1 individuals using ART had lower subgingival EBV loads than those not using it (5.03 log10 copies/mL, and 7.14 log10 copies/mL, respectively; P = .0348). CONCLUSIONS: Subgingival sites, especially the periodontal pockets, are suggested to act as a reservoir of EBV in HIV+ individuals. Therefore, the identification of latent EBV infections in this easily accessible site might help to improve quality of life in patients with HIV by maintaining oral/periodontal health. In addition it might encourage new approaches in investigating EBV-associated disorders in HIV+ patients.

Avaliação clínica e microbiológica de prótese total deidosos em instituições assistenciais / Assessment clinical and microbiological of complete dentures in aged from health care institution

Introdução: O Brasil possui mais de sete milhões de idosos desdentados e a prótese totaldentária ainda é a principal forma de reabilitar esses indivíduos. O objetivo deste trabalhofoi avaliar clínica e microbiologicamente próteses totais usadas por idosos de instituiçõesassistenciais. Métodos - Foram avaliados 55 idosos, acima de 60 anos de idade, portadoresde prótese total superior. Foi utilizado um questionário para coleta de informações. Exameclínico do palato foi realizado para detecção de lesões inflamatórias, seguido de quantificaçãoda placa bacteriana visível e colheita de biofilme microbiano da região de palato da prótesetotal superior. As amostras de biofilme foram processadas para cultivo de leveduras do gêneroCandida. Resultados - O tempo de uso da prótese foi superior a 10 anos, com conservaçãoinsatisfatória. A higienização prevalente foi o uso de escova dental com dentifrício, comfrequência de três vezes ao dia, mas 56,4% das próteses apresentaram higienização precária.Lesões inflamatórias estavam presentes em 72,7% dos idosos e a colonização por Candidafoi detectada em 60% das próteses. O estudo das correlações revelou significância estatísticado padrão de higienização com a colonização de levedura e também com a presença de lesãono palato, enquanto o aumento no número de colônias de espécies de Candida correlacionou--se com lesões inflamatórias no palato. Conclusão - Os idosos são portadores de prótesesantigas e deterioradas, com higienização inadequada. As lesões inflamatórias fúngicas sãoprevalentes no palato. Faltam informações adequadas e programas de atendimento ao idosopara garantir melhores condições de saúde bucal.

Introduction: There are more than seven millions of completely edentulous aged in Braziland complete dentures are still the main way of rehabilitating these individuals. The purposeof this study was to appraise clinically and microbiologically complete dentures in aged fromhealth care institutions. Methods - 55 aged have been evaluated, over 60 years old, usingupper complete dentures. Questionnaire is used for take information about patients. Palateclinical examinations were made in order to detect de inflammatory lesions, in accordancewith the quantification of the visible bacterial plaque and collection of microbial biofilmof the palate region from upper complete denture. The biofilm samples were processed forcultivation of yeasts of Candida. Results - Used time of the prosthesis was above 10 years,which presented an unpleasant state of conservation. The common form of hygienization wasthe toothbrush with dentifrice, with frequency of three times a day, but 56,4% of the prosthesispresented precarious hygienization. The inflammatory lesions were present in 72,7% ofthe aged and the colonization by Candida was detected in 60% of the prosthesis. The studyof the correlations revealed statistical significance of the hygienization pattern along withthe colonization of yeasts and also with the presence of inflammatory lesions in the palate.Conclusion - The aged are users of old and damaged prosthesis, with inadequate hygienization.The colonization of Candida is prevalent, as well as the inflammatory lesions. Thereis a lack of appropriate information and assisting programs to aged in order to guaranteebetter mouth health conditions.