Leachate derived humic-like substances drive the variation in microbial communities in landfill-affected groundwater.

Landfills are commonly used for waste disposal in many countries, and pose a significant threat of groundwater contamination. Dissolved organic matter (DOM) plays a crucial role as a carbon and energy source, supporting the growth and activity of microorganisms. However, the changes in the DOM signature and microbial community composition in landfill-affected groundwater and their bidirectional relationships remain inadequately explored. Herein, we showed that DOM originating from more recent landfills mainly comprises microbially produced substances resembling tryptophan and tyrosine. Conversely, DOM originating from older landfills predominantly comprises fulvic-like and humic-like compounds. Leachate leakage increases microbial diversity and richness and facilitates the transfer of foreign bacteria from landfills to groundwater, thereby increasing the vulnerability of the microbial ecosystem in groundwater. Deterministic processes dominated the assembly of the groundwater microbial community, while stochastic processes accounted for an increased proportion of the microbial community in the old landfills. The dominant phyla observed in groundwater were Proteobacteria, Bacteroidota, and Actinobacteriota, and humic-like substances play a crucial role in driving the variation in microbial communities in landfill-affected groundwater. Predictions using PICRUSt2 suggested significant associations between various metabolic pathways and microbial communities, with the Kyoto Encyclopedia of Genes and Genomes pathway "Metabolism" being the most predominant. The findings contribute to advancing our understanding of the transformation of DOM and its interplay with microbial communities and can serve as a scientific reference for decision-making regarding groundwater pollution monitoring and remediation.

Long noncoding RNA DANCR expression and its predictive value in patients with atherosclerosis.

Long noncoding RNAs (lncRNAs) act crucial roles in the progression of vascular diseases, including atherosclerosis. This study aims to investigate the expression levels of the atherosclerosis-associated lncRNA DANCR in patients diagnosed with atherosclerosis and whether its abnormal expression affects the progress of atherosclerosis. The expression of DANCR in the serum samples of all study participants was quantified using RT-qPCR. Then, the predictive capacities of DANCR for the detection of atherosclerosis patients were evaluated via receiver operating characteristic (ROC) curve analysis. The effects of DANCR on vascular smooth muscle cells (VSMCs) proliferation and migration were then explored using cell counting kit-8 (CCK-8) and Transwell migration assays. The DANCR exhibited increased expression trends in patients with atherosclerosis than healthy controls. Moreover, there were differences in the levels of low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), and C-reactive protein (CRP) between the healthy controls and atherosclerosis patients. The DANCR expression was positively correlated with serum LDL-C, Hcy, and CRP levels. DANCR expression could distinguish patients with atherosclerosis from healthy individuals with a high area under the ROC curve (AUC), sensitivity, and specificity. Additionally, knockdown of DANCR weakened the proliferative abilities and migration capacities of VSMCs. It was also shown that DANCR could compete with miR-335-5p binding. Herein, it appears that the LncRNA DANCR was closely associated with the progression of atherosclerosis by targeting miR-335-5p, which might be a potential detective predictor and target for the treatment of atherosclerosis.

Efficient Degradation of 2,4-Dichlorophenol on Activation of Peroxymonosulfate Mediated by MnO2.

Sulfate radical based-advanced oxidation process has received increasing interest in the remediation of wastewater and contaminated soil. In this study, degradation of 2, 4-dichlorophenol (2, 4-DCP) was investigated over peroxymonosulfate (PMS) activation by MnO2, which was prepared by liquid-phase oxidation method. The prepared MnO2 was characterized by transition electron microscopy, X-ray diffraction, N2 adsorption-desorption, and X-ray photoelectron spectroscopy. Characterization results showed that α-MnO2 exhibited the highest surface area and Mn (III) content. The PMS activation by MnO2 in 2, 4-DCP degradation followed the order of α-MnO2 > γ-MnO2 > ß-MnO2, which is dependent on the properties of MnO2 including crystal structure, surface area and Mn (III) content. Influences of initial concentration of 2, 4-DCP, PMS and MnO2 dosage, pH and co-existing inorganic ions on the degradation were examined. Electron paramagnetic resonance (EPR) and quenching experiments with ethanol and tert-butanol suggested that sulfate radicals were the dominant radicals in the process. Findings in this study indicated that α-MnO2 was an attractive catalyst for activation of PMS to degrade 2, 4-DCP in aqueous solution.

Efficient transformation of DDT with peroxymonosulfate activation by different crystallographic MnO2.

In this study, three different structures of MnO2 were synthesized and used to activate peroxymonosulfate (PMS) for the degradation of DDT in aqueous solutions. It was found that DDT was efficiently degraded in the MnO2/PMS system and the degradation rate was dependent on the properties of MnO2 including crystal structure (followed the order: α-MnO2 > Î³-MnO2 > ß-MnO2), surface area and Mn(III) content. Sulfate radicals (SO4-) was primarily responsible for the degradation of DDT based on the results of electron paramagnetic resonance (EPR) and quenching experiments. The degradation of DDT was suppressed at alkaline pH because the formation of SO4- was inhibited. The results of GC-MS indicated that dichlorobenzophenone, 4-chlorobenzoic acid and benzylalcohol were the dominant intermediates for DDT degradation. The possible pathways of DDT degradation were proposed according to the identified products.

Efficient removal of three dyes using porous covalent triazine frameworks: adsorption mechanism and role of pore distribution.

Dyes are widely used in production and life. In this study, porous covalent triazine frameworks (CTFs) were synthesized and the adsorption behavior for three dyes was investigated by batch adsorption experiments. CTFs were characterized by various spectroscopic techniques for structure, porosity and surface properties. Several possible adsorption mechanisms were proposed including pore-filling, electrostatic attraction and hydrogen bonding interaction with the triazine structure of CTFs. The mechanisms were further verified by the pore size distribution and pH dependence. Additionally, CTFDCBP displayed stronger adsorption affinity and faster adsorption kinetics for dyes, because of the wide pore size distribution. This study provides a new insight into the mesoporous CTFs, which exhibit great potential as an effective adsorbent for dye removal.

Proteomic profiling uncovered the cytosolic superoxide dismutase BsSOD1 associated with plant defence in the herbal orchid Bletilla striata.

The herbal orchid Bletilla striata (Thunb.) Rchb.f. has a long cultivation history and has been widely used in medicines and cosmetics. The fungal infection leaf blight (LB) seriously threatens B. striata cultivation. Here, we systemically collected wild B. striata accessions and isolated the accessions with strong resistance against LB. We carried out proteomic profiling analysis of LB-resistant and LB-susceptible accessions, and identified a large number of differentially expressed proteins with significant gene ontology enrichment for 'oxidoreductase activity.' Of the proteins identified in the reactive oxygen species signalling pathway, the protein abundance of the Cu-Zn superoxide dismutase BsSOD1 and its gene expression level were higher in LB-resistant accessions than in LB-susceptible lines. Transient expression of the dismutase fused with yellow fluorescent protein determined that its subcellular localisation is in the cytoplasm. Our study provides new insights into the molecular markers associated with fungal infection in B. striata.

Comparative transcriptomics characterized the distinct biosynthetic abilities of terpenoid and paeoniflorin biosynthesis in herbaceous peony strains.

The herbaceous peony (Paeonia lactiflora Pall.) is a perennial flowering plant of the Paeoniaceae species that is widely cultivated for medical and ornamental uses. The monoterpene glucoside paeoniflorin and its derivatives are the active compounds of the P. lactiflora roots. However, the gene regulation pathways associated with monoterpene and paeoniflorin biosynthesis in P. lactiflora are still unclear. Here, we selected three genotypes of P. lactiflora with distinct morphologic features and chemical compositions that were a result of long-term reproductive isolation. We performed an RNA-sequencing experiment to profile the transcriptome changes of the shoots and roots. Using de novo assembly analysis, we identified 36,264 unigenes, including 521 genes responsible for encoding transcription factors. We also identified 28,925 unigenes that were differentially expressed in different organs and/or genotypes. Pathway enrichment analysis showed that the P. lactiflora unigenes were significantly overrepresented in several secondary metabolite biosynthesis pathways. We identified and profiled 33 genes responsible for encoding the enzymescontrolling the major catalytic reactions in the terpenoid backbone and in monoterpenoid biosynthesis. Our study identified the candidate genes in the terpenoid biosynthesis pathways, providing useful information for metabolic engineering of P. lactiflora intended for pharmaceutical uses and facilitating the development of strategies to improve marker-assist P. lactiflora in the future.

Construction and significance analysis of the MicroRNA expression profile of Hemerocallis fulva at low temperature.

MicroRNA (miRNA) identification was performed in Hemerocallis fulva by high-throughput sequencing in combination with bioinformatics prediction. A total of 14,843,184 and 16,072,575 RNA sequences were explored under normal and low temperature conditions, respectively. There was a significant difference in RNAs species and quantity between the two samples. Of all the miRNAs, 26 were significantly upregulated and 30 were significantly downregulated, while nine were either significantly upregulated or downregulated under low-temperature stress. Twenty-one highly expressed miRNA families were screened in at least six species. The number of miRNA families was very similar between monocotyledons and dicotyledons, and only a few were more frequently found in monocotyledons.