Association between Toxoplasma gondii and gestational diabetes with regard to serum leptin and tumor necrosis factor alpha, a preliminary study.

Gestational diabetes (GDM), the onset of any degree of glucose intolerance during pregnancy, increases a wide range of adverse health outcomes for both the mother and the fetus. The aim of the present study was to evaluate the association of Toxoplasma gondii infection with GDM in a case-control study with regard to the levels of leptin and tumor necrosis factor alpha (TNF-α) as two inflammatory biomarkers. Fifty-one pregnant diabetic cases and 109 controls were selected from a prenatal care clinic of a general hospital in Shiraz, southern Iran during July-November 2020. Cases and controls were similar in age, gestational age and number of parturitions. The presence of IgG antibodies against T. gondii, and serum concentrations of leptin and TNF-α were determined by ELISA. Anti-Toxoplasma antibodies were detected in 25 subjects (15.6 %, 95 % CI: 9.9-21.3). Nine (18 %) diabetic cases were infected with Toxoplasma compared to 16 (15 %) healthy controls (P = 0.63). Level of leptin was higher (P = 0.07) while TNF-α was lower in diabetic cases compared to healthy controls (P = 0.08). When subjects were classified according to the combination of GDM and T. gondii, leptin was significantly lower in healthy (non-diabetic, non-infected) subjects compared to diabetics (P = 0.026), and TNF-α was higher in healthy subjects compared to Toxoplasma-infected diabetics (P = 0.032). These findings can be interpreted as both comorbidities being individually associated with increasing serum leptin and decreasing TNF-α concentrations, with modifying effects on each other. The present study opens a new perspective on GDM and its complex pathophysiological mechanism. Future research in this area is needed to better understand the underlying pathway for the development of GDM and the role of T. gondii and inflammatory biomarkers.

Knowledge, attitudes and practices of dog and cat owners toward zoonotic diseases in Shiraz, southern Iran.

There are increasing concerns about the role of companion animals as reservoirs for zoonotic diseases (ZD). Therefore, knowledge, attitudes and practices of pet owners in Shiraz, southern Iran about ZD were evaluated. Two-hundred pet owners referring to veterinary clinics, completed a structured questionnaire. Mean age of owners was 34 (±12) years, more than half of owners reported they had heard the term "zoonosis", nearly half had some information about ZD, and veterinarians were their main source of information. Two-thirds of respondents had history of bite or scratching. Participants had satisfactory knowledge regarding high risk groups for ZD, however, nearly half did not know that scratching by animal is a route for contracting ZD and 92 % thought that animal vaccination could prevent a large number of ZD. More than half said when their pet is apparently healthy, they do not concern about ZD, and almost all agreed that it is the responsibility of veterinarians to provide sufficient information about ZD for their clients. Routine vaccinations was practiced by 83 % of the owners, and 79 % washed their hands after contact with pet. Using gloves when emptying feces container and annual stool examination were the least common practices. Education was significantly associated with total knowledge (P = 0.007) and practices scores (P = 0.001), hearing the term "zoonoses" (OR= 2.2, P = 0.015) and concern for ZD in apparently healthy animals (OR= 3.1, P = 0.001). Total practices score was also significantly higher in dog owners (P = 0.005). Age and gender did not show significant associations with any item. This study established baseline estimates for knowledge, attitudes and practices of pet owners toward ZD in the region. Educating pet owners about the interconnection between animal and human health, preferably by veterinarians is highly warranted for prevention of ZD.

Survey of Salmonella in commercial broiler farms in Shiraz, southern Iran.

Salmonella is one of the major causes of food-borne diseases, worldwide. The aim of the present study was to describe the prevalence of Salmonella and to employ a polymerase chain reaction (PCR) assay to confirm the presence of Salmonella Enteritidis and Salmonella Typhimurium in the broiler chicken farms in Shiraz, southern Iran. In addition, risk factors for the presence of Salmonella spp. at farm and flock levels were investigated. Fecal samples were collected from 22 broiler farms, including 35 broiler flocks. Conventional culture methods were used for Salmonella isolation, and the suspected isolates were confirmed by PCR with Salmonella specific primer (invA). Subsequently, PCR was performed to identify S. Enteritidis and S. Typhimurium, using IE-1 and Flic-C primers, respectively. Information for farms and flocks was collected using a questionnaire. Twelve poultry flocks from eight farms were positive for Salmonella. The estimated prevalence of Salmonella was 36.4% at farm level and 34.3% at flock level. Based on the results of PCR, four farms were infected with S. Enteritidis, two farms with S. Typhimurium and one farm with both serovars, concurrently. Statistical analysis using generalized estimating equations showed that at flock level, odds of Salmonella presence increased when the number of chickens was more than 15000 (OR = 13.2, P = 0.023), and an increased odds of Salmonella was found for flocks in which antibiotics were used at sub-therapeutic or therapeutic doses during the rearing period (OR = 19.6, P = 0.003). At the farm level, there was a marginal association between Salmonella and using nipple drinker (OR = 0.08, P = 0.07) and keeping dogs on the farm (OR = 8.9, P = 0.06) by logistic regression analysis. In conclusion, Salmonella spp. including S. Enteritidis and S. Typhimurium are prevalent in the poultry flocks in the region. Considering the results and the fact that the flock size and its surrogate marker, stocking density are among the most consistently identified risk factors for Salmonella in the literature, production cycles with the appropriate number of chicks and proper stocking density are recommended. In addition, careful monitoring and prudent use of antibiotics in poultry farms could be practiced to control this human pathogen in preharvest poultry operations.

Neospora caninum in aborted bovine fetuses in Iran: a systematic review and meta-analysis.

The aim of the present study is to systematically review the studies on Neospora caninum infection in aborted bovine fetuses in Iran. Search for all published reports on N. caninum in Iran was performed from inception until January 2020. Major English (PubMed, Scopus and Science Direct) and Persian (SID, Iranmedex, and Magiran) electronic database sources were used. Finally, 16 articles were assessed for eligibility and 12 articles comprising 20 independent studies were selected for inclusion in the meta-analysis. Due to the statistically significant heterogeneity (Q=149.12, df=19, P<0.001 and I2=87.26), the random effect model was used for meta-analysis. Prevalence of N. caninum infection in 1239 aborted bovine fetuses with 351 positive ones was 34.8% (95% CI: 26.5­44.0%). Sample size was categorized as < 50 and ≥ 50 and subgroup meta-analysis was performed to compensate for the loss of precision due to inclusion of small size studies. The result showed that overall prevalence of infection in the latter group (23.1%) is significantly lower than in the first category (45.7%). It may be suggested that the subgroup with large sample size would be more precise and conservative approximation of the infection at the country level. The present meta-analysis confirms the previous findings regarding the importance of N. caninum as a major abortion agent in cattle industry in Iran and the need for appropriate action toward prevention and control of this parasite.

Additive effect of glutathione S-transferase T1 active genotype and infection with Toxoplasma gondii for increasing the risk of schizophrenia.

PURPOSE: To determine if Toxoplasma gondii (T. gondii) infection may play a role in the development of schizophrenia in genetically susceptible persons with regard to genes encoding glutathione S-transferase T1 (GSTT1) and M1 (GSTM1). METHODS: A total of 78 cases with psychiatric diagnosis of schizophrenia were compared with 91 healthy controls. For detection of IgG antibodies, enzyme-linked immunosorbent assay was used. Genotyping of GSTM1 and GSTT1 was performed by multiplex PCR. Chi-square and logistic regression were used for statistical analyses. RESULTS: A higher frequency of the GSTT1 active gene in schizophrenic patients was observed. When risk categories based on the combination of T. gondii status and GSTs polymorphisms were compared, risk of schizophrenia increased in T. gondii positive/GSTT1 absent subjects (OR = 4.75, p = 0.05) compared with T. gondii negative/GSTT1 absent group. When T. gondii positive subjects had the GSTT1 active genotype, the risk increased linearly (OR = 10.20, p < 0.001). Odds ratio in T. gondii positive groups were almost the same in combination with the GSTM1 active genotype (OR = 4.45, p = 0.003) or null genotype (OR = 4.37, p = 0.006). CONCLUSIONS: Our results showed an additive effect for T. gondii and GSTT1 active genotype as risk factors for schizophrenia in Iranian population. This is a small pilot study and replicating the study with larger groups of patients in multinational investigation to clarify these findings is recommended.

Bovine neosporosis in Iran: A systematic review and meta-analysis.

Neospora caninum is an apicomplexan parasite with worldwide distribution, which primarily causes disease in dogs and cattle. It has been recognized as an important cause of epidemic and endemic abortion in cattle around the world. There is no study to demonstrate the country level picture of the disease in Iran and estimate the extent of the problem, quantitatively. Therefore, the aim of the present study was to systematically review the observational studies on bovine neosporosis in Iran and estimate an overall serologic prevalence of the infection in the cattle population. Search for all published reports on N. caninum in Iran was conducted from inception until September 2018. Major English (PubMed, Scopus and Science Direct) and Persian (IranDoc, SID, Iranmedex, and Magiran) electronic database sources were used for this study. After removing duplicated articles, 128 documents remained. Unrelated articles (89) were excluded after primary screening and 39 articles were evaluated for eligibility. Finally, 37 studies from 33 articles were recognized as eligible and were included in the present work. The author screened the studies and extracted the data twice, carefully. Based on the meta-analysis, the overall serologic prevalence of N. caninum in 10,967 cattle with 2619 positive ones was 23.6 % with 95 % CI: 19.8-27.9 %. The random effect model was used for meta-analysis because the Cochran Q value (795.10, df = 36, P < 0.001) and Higgins index (I2 = 95.47 %) showed statistically significant heterogeneity. In subgroup meta-analysis, abortion status of the animal and geographical location of the study showed significant association with serologic prevalence, while age and breed did not have significant associations. No publication bias was detected, and sensitivity analysis showed stability of the overall estimate after excluding any individual study. It could be concluded that N. caninum is widespread in cattle population in Iran and the time is right to implement national level educational and control programs for it.

Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia.

Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of Anaplasma spp., Bartonella spp., hemotropic Mycoplasma spp., Babesia spp., and Hepatozoon spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with Anaplasma platys (57.1%), Babesia vogeli (30%), Mycoplasma haemocanis (15.7%), and Bartonella henselae (1.4%), and cats were infected with Mycoplasma haemofelis (13.6%), Candidatus Mycoplasma haemominutum (13.6%), B. henselae (9.2%), and A. platys (2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with A. platys and B. vogeli was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.

Effect of L-Carnitine Supplementation on Apelin and Apelin Receptor (Apj) Expression in Cardiac Muscle of Obese Diabetic Rats.

OBJECTIVE: L-carnitine (LC) has been shown to protect cardiac metabolism in diabetes patients with cardiovascular diseases (CVDs). Apelin, a newly discovered adipocytokines, is an important regulator of cardiac muscle function; however, the role of the level of expression of Apelin axis in improvement of cardiac function by LC in diabetic patients, is not clear. In the present study, obese insulin-resistant rats were used to determine the effect of LC, when given orally with a high-calorie diet, on Apelin and Apelin receptor (Apj) expression in cardiac muscle. MATERIALS AND METHODS: In this experimental study, rats were fed with high-fat/high-carbohydrate diet for five weeks and subsequently were injected with streptozotocin 30 mg/kg for induction of obesity and insulin resistance. After confirming the induction of diabetes (serum glucose above 7.5 mmol/L), the animals received LC 300 mg/kg in drinking water for 28 days. On days 0, 14 and 28 after treatment, cardiac Apelin and Apj gene expression was evaluated by real time polymerase chain reaction (PCR) analysis. Serum levels of insulin, Apelin, glucose, tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß) and the homeostasis model assessment of insulin resistance (HOMA-IR) were also measured using commercial kits. RESULTS: Cardiac Apelin and Apj expression and serum Apelin were increased in obese rats, while LC supplementation decreased the serum levels of Apelin and down-regulated Apelin and Apj expression in cardiac muscle. These changes were associated with reduced insulin resistance markers and serum inflammatory factors and improved lipid profile. CONCLUSION: We concluded that LC supplementation could attenuate the over-expression of Apelin axis in heart of diabetic rats, a novel mechanism by which LC improves cardiovascular complications in diabetic patients.

Association of Neospora caninum with reproductive performance in dairy cows: A prospective study from Iran.

There is no prospective study from Iran to estimate the direct risk of Neospora caninum for pregnancy loss or reproductive factors. In addition, there is no report in the literature concerning the association of N. caninum with dystocia and sex of calves. Therefore, this study was conducted on a group of dairy cows in a large intensive production system during 2011 to 2013 in southern Iran to evaluate the impact of neosporosis on reproductive performance. A total of 253 cows which were diagnosed as pregnant during the first six months of the study were followed until calving or abortion. Reproductive data were collected and N. caninum serostatus was determined using ELISA. To investigate the association of abortion with N. caninum, survival analysis was performed using Cox proportional hazard model. The N. caninum seroprevalence in the study group was 30.40% (95% CI: 27.40, 36.10). The overall abortion rate was 12.25%, significantly higher in seropositive animals (20.80%) than seronegative ones (8.50%). Results of Cox model showed that serostatus of animal for N. caninum and season had significant associations with abortion (p < 0.01). Neospora caninum did not show significant association with other factors such as dystocia and sex of calves. In conclusion, neosporosis is responsible for 12.00% excess abortion risk in infected group and more than 30.00% of abortions could be preventable by control of Neospora in study population. Therefore, control of N. caninum would reduce the economic losses caused by parasite mainly due to pregnancy loss.

Evaluation of blood monocyte and lymphocyte population in broiler chicken after vaccination and experimental challenge with Newcastle disease virus.

In the present study, after vaccination and challenge with Newcastle disease virus, changes in the population of blood monocytes and lymphocytes of broiler chickens were evaluated using flow cytometry. 300 apparently healthy 1-day-old Cobb broiler chicks were divided randomly into four experimental groups (n=75). At 20days of age the chicks in group 1 and 2 were vaccinated with live B1 ND vaccine. Those in group 2 were additionally injected with a killed vaccine simultaneously and group 3 chicks received only the adjuvant of the killed vaccine. The birds in groups 1, 2 and 3 were challenged with a velogenic ND virus and those in group 4 were treated as control. Sampling was done on days 1,2,3,7 after vaccination and also on 1, 2, 3,7,14, 21 post challenge days. In this study percentage of B cell population was increased after vaccination and challenge in vaccinated birds, but CD3+ cells were decreased after vaccination and challenge, which showed B cells have more expansion than T cells. The CD4+ cell percentage in vaccinated birds was always lower than control birds. However, the percentage of CD8+ cells in vaccinated birds was increased. Results indicate increased CMI with the live NDV vaccination. In this study CD4/CD8 ratio in control birds was about 1.5 at 30days of age and it was slightly lower in vaccinated and challenged birds. The percentage of monocytes in vaccinated birds was significantly higher than control birds from 3days post vaccination to the end of the experiment.

Association of Toxoplasma gondii infection with schizophrenia and its relationship with suicide attempts in these patients.

OBJECTIVES: To investigate the association between schizophrenia and Toxoplasma gondii, and to assess the association of infection with suicide attempts and age of onset of schizophrenia in these patients. METHODS: Case-control study Fars Province, southern Iran. Cases were individuals with psychiatric diagnosis of schizophrenia as per Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) criteria. Controls were healthy blood donors, frequency-matched with patients according to age and sex. For the detection of IgG antibodies, enzyme-linked immunosorbent assay (ELISA) was used. Data about demographic information in all subjects and duration of illness and history of suicide attempts in patients with schizophrenia were collected using a brief questionnaire and hospital records. Chi-square test and multivariable logistic regression were used for statistical analyses. RESULTS: Among 99 cases, 42 individuals (42%) were positive for T. gondii antibody, vs. 41 (27%) among 152 controls (OR = 2, 95% CI: 1.2-3.4, P = 0.012). We compared the suicide attempts in patients with schizophrenia based on their T. gondii serologic status. There was a lower rate of suicide attempts in seropositive male patients than seronegative ones (OR = 0.3, 95% CI: 0.1-0.97, P = 0.04). Age of onset of schizophrenia did not differ between T. gondii-infected and non-infected patients. CONCLUSIONS: These findings may have implications for schizophrenia and suicide prevention programmes. However, clearly further studies are required to confirm them.

Impact of FMD outbreak on milk production and heifers' growth on a dairy herd in southern Iran.

Foot and mouth disease is endemic in Middle Eastern countries including Iran but its impact is poorly characterized. The present study was conducted to evaluate the impact of FMD outbreak on milk production and heifers' growth in an industrial dairy herd located in Fars province, southern Iran. Data about individual milk production, heifers' growth and total daily milk (sold for manufacturing), its fat and protein content and somatic cell counts were collected from the herd database. Based on the results of the linear mixed models, a significant decline in individual milk production after the outbreak was observed compared with before the outbreak. There was a total reduction of 8.0 and 4.7% in mean daily milk production per cow after the outbreak when compared with before (over a 42days outbreak period) in lactation one (P<0.001) and lactation ≥2 cows (P=0.024), respectively. The total daily milk (P=0.027) and protein (P=0.002) showed significant decline during the outbreak period. The fat content decreased after the outbreak (P=0.014). Somatic cell counts did not show significant changes. The recorded heifers' weights (4-17 months of age) showed 7.1kg decrease after the outbreak in comparison with the period before that (P<0.001). In conclusion, we observed a negative impact of FMD outbreak on milk production and heifers' growth in study herd. The impact on daily milk production was less than the values reported previously. This difference could be attributed at least partly to differences in livestock genetics and management practices. Lower growth rate of heifers after the outbreak period could potentially extend the age at first calving. It is suggested that farmers are educated on awareness and preparation for infectious disease outbreaks and to practice good management routines that could potentially reduce the economic impact of these diseases.

Gastroscopic Study of Meloxicam, Tramadol, and Their Combined Administration on the Development of Gastric Injuries in Dogs.

Opioid and non-steroid anti-inflammatory drugs (NSAIDs) are commonly used to manage post-operative pain that may be chronically extended. Although NSAIDs have been approved for their analgesic effects in canine, they are mostly known to be associated with side effects, particularly gastric ulcers. In the present study, we evaluated short-term co-administration of meloxicam and tramadol to see if this could induce more gastric ulcers than that observed when using these drugs individually. Twenty adult mixed domestic dogs weighing 10 to 20 kg of both sexes, were randomly assigned to four groups of five dogs. In the control group, placebo was administered orally (twice a day), whereas the test groups received tramadol (per OS) twice a day, meloxicam (per OS) daily, and a combination of tramadol (twice a day) and meloxicam (daily) for ten days, respectively. The animals were evaluated for gastric injuries on days 0, 6, 11, 16 and 21 by endoscopy under general anesthesia. Clinical signs of all animals including fecal consistency, appetite, mental and hydration statuses, tempreture, heart rate and respiratory rate were evaluated daily. Based on our results, there was no significant difference between the experimental and control groups. In conclusion, our study demonstrated that a 10-day oral coadministration of tramadol and meloxicam had no deleterious effects on general health status and gastric mucosa in dogs.

Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats.

Methyl tertiary butyl ether (MTBE) is an oxygenated fuel additive which has been used widely in many parts of the world. This experiment was performed to determine the effect of MTBE on offspring sex ratio, sex hormones and antioxidant enzymes. A total of 20 adult Sprague-Dawley male rats were divided into four groups and received 0, 400, 800 and 1600 mg/kg/day MTBE by gavages for 30 consecutive days. At the end of the experiment, blood samples were taken for determination of sex hormones and antioxidant enzymes. Then, male rats were mated with healthy unexposed female rats and sex of offspring was determined after birth. Sex ratio was 0.48, 0.50, 0.43 and 0.50 in 0, 400, 800 and 1600 mg/kg/day MTBE groups, respectively (P = 0.91). There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively). No changes were observed for superoxide dismutase. However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016). The present study showed that paternal exposure to oral MTBE has no effect on offspring sex ratio; while, MTBE exposure could exert dose-dependent changes in serum testosterone and LH in treatment groups. The results of the present study, need to be clarified in the future studies.

Prevalence and risk factors for Salmonella spp. colonization in broiler flocks in Shiraz, southern Iran.

Salmonella spp. are important food borne pathogens worldwide that frequently infect poultry flocks. This cross-sectional study was conducted to determine the prevalence of Salmonella spp. colonization in broiler flocks in Shiraz (southern Iran) and to find the possible association of infection status with some potential risk factors including vaccination program and use of antibiotics. During October 2009 to April 2010, a total of 40 broiler flocks were selected in slaughterhouse and 20 cloacae contents were collected from each flock. Every five cloacae contents were pooled and investigated for Salmonella spp. using appropriate culture methods. The flock was considered positive if any of the pooled samples turned positive in culture. Statistical analysis was performed using multiple logistic regression. Nine out of 40 flocks (22.50%, 95% CI: 9-36) were positive for Salmonella spp. colonization. Nearly 75.00% of flock owners reported that they used antibiotics during production period, more frequently fluoroquinolones, combination of trimethoprim-sulfonamides (TMP/SU) and tetracycline. Nearly 60.00% of the flocks which had used TMP/SU were positive for Salmonella spp. compared with 10.00% of the flocks which did not use this antibiotic (p = 0.006). Increasing flock age was associated with a decreased chance of Salmonella spp. detection (p = 0.003). In flocks which received infectious bronchitis vaccine, 36.00% were positive for Salmonella spp. whereas this was 15.00% for flocks which did not receive this vaccine (p = 0.08). Careful monitoring of antibiotics use and further studies to determine the most appropriate vaccination program in the field is recommended.

Identifying relationships among acute phase proteins (haptoglobin, serum amyloid A, fibrinogen, ceruloplasmin) and clinical findings in dairy calf diarrhea.

The acute phase response is a nonspecific inflammatory reaction of the host that occurs shortly after any tissue injury. The response includes changes in the concentration of plasma proteins called acute phase proteins (APPs). Calf diarrhea is an important disease that occurs in association with the interaction of various infectious agents and calf susceptibility. The economic losses is associated with death loss and treatment costs, reduction of live weight gain, and reduction of productive life span, which may be considerable. The aim of the present study was to identify relationships among APPs in calves with diarrhea in the different clinical features. Holstein calves (50) within 1 day to 4 months old with signs of diarrhea and healthy calves (40) with similar age and sex were selected. Standard clinical examinations and also dehydration degree were carried out on each calf and were recorded. Calves with clinical signs of diarrhea were divided in different groups based on the severity of the clinical findings, fever and degree of dehydration. Blood samples were taken from the jugular vein from all calves into vacutainers containing ethylenediaminetetraacetic acid (EDTA) for separating plasma and without EDTA for serum biochemical analysis. APP [haptoglobin (Hp), serum amyloid A (SAA), fibrinogen (Fib), and ceruloplasmin (Cp)] concentrations were measured using validated standard methods. The results indicated a significant increases in APPs in diarrheic calves which was most obvious in Hp and SAA (P < 0.001). Calves with severe clinical signs of diarrhea had a significant increases in their Hp and SAA (P < 0.001) compared to calves with moderate or without systemic clinical signs. Diarrheic calves with fever compared to diarrheic calves without fever had a significant increases in their Hp and SAA (P < 0.01). Also, diarrheic calves with severe dehydration compared to diarrheic calves with mild and moderate dehydration had significant increases in Hp and SAA (P < 0.05), and these parameters (Hp, SAA, Fib, and Cp) among calves with mild and moderate dehydration had no significant changes. Our results indicated that monitoring the APP responses in diarrheic calves with different clinical signs could be useful as prognostic tools and facilitate treatment decisions.

Prevalence and risk factors for Listeria monocytogenes in broiler flocks in Shiraz, southern Iran.

Listeria monocytogenes has been identified as an important foodborne pathogen in recent years. In humans, it most commonly affects pregnant women, neonates, children, elderly people, and persons with a suppressed immune system. It could contaminate both raw and cooked meat and poultry products. Studies regarding prevalence and risk factors of L. monocytogenes in broilers flocks are limited. Therefore, the present study was conducted to determine the prevalence and risk factors for L. monocytogenes in poultry flocks in Shiraz, southern Iran. During August to September 2009, in total, 100 broiler flocks were selected at slaughter, and 21 specimens were collected from cloacal samples from each flock. Polymerase chain reaction (PCR) was performed on the samples enriched in buffered Listeria enrichment broth (BLEB), using specific primers. Furthermore, enriched samples in BLEB and/or BLEB treated with 5% KOH were subcultured on Palcam medium. Data about farm and flocks were collected using a structured questionnaire. The prevalence of L. monocytogenes was 7% (95% CI, 2-12%) and 1% using PCR and culture, respectively. Results showed that using antibiotics during rearing period was dramatically reduced the rate of isolation (odds ratio [OR]=0.07, p=0.03), whereas house capacity of more than 10,000 birds (OR=24.03, p=0.04) and number of houses (OR=2, p=0.02) significantly increased the prevalence. The correlation between poor management of large poultry flocks and increasing the risk of contamination was more likely due to the recontamination of cooked poultry/undercooking or cross-contamination of other ready-to-eat foods.

Association of Mycobacterium avium subspecies paratuberculosis infection with milk production and calving interval in Iranian Holsteins.

There are inconsistent results for the association of Mycobacterium avium subspecies paratuberculosis (MAP) infection with production and reproduction in dairy cows. Determination of these associations in each region is essential to encourage participation of dairy cattle producers in disease control programs. This study was conducted in Shiraz, southern Iran, to quantify the association of subclinical MAP infection with 305-day milk production and calving interval in Iranian Holsteins. A total of 21 dairy herds were selected for the study and in each herd, quarter milk samples were collected from ten to 12 dairy cows for PCR analysis. Data about parity, calving interval, length of lactation period, total milk production and 305-day milk production were also provided for each animal. Overall, 252 individual milk samples were collected. Herd- and individual-level prevalence of MAP infection were 23.8% (95% CI, 6.2-41.4%) and 3.2% (95% CI, 1.3-5.1%), respectively based on IS900 nested PCR. The results for 305-day milk production revealed a 248 kg reduction in positive cows compared with negative ones (P = 0.009). When cows from positive herds were compared with cows from negative herds, a 335-kg reduction in 305-day milk production (P = 0.005) and a 30-day increase in calving interval (P = 0.057) were observed in the former group. These findings support the previous results that paratuberculosis infection is negatively associated with the performance of the animals.

Causes of culling in dairy cows and its relation to age at culling and interval from calving in Shiraz, Southern Iran.

This study was designed to investigate causes of culling in industrial dairy herds in Fars province and to describe the pattern of reason-specific culling with respect to age of animal and interval from calving to culling. A total number of 9 dairy herds were selected for the study and information about culling reasons, birth date, last calving date and culling date was collected for culled cows during 2005-2006. Infertility (32.6% of all culls) was the most prevalent reason of culling followed by mastitis (6.5%). The time interval from last calving to culling averaged 240 days (SD = 176) and nearly 28% of cows were culled in the first 100 days after calving. Mean age of animals at culling was 6 years (SD = 2.7) and median was 5.7 years. In Cox proportional hazard model for calving to culling interval, infertility (hazard ratio [HR] = 0.26) showed lower risk whereas mastitis (HR = 2.40), left displaced abomasum (HR = 2.60) and peripartum problems (HR = 2.60) had higher risk of culling compared with voluntary cull. In the Cox model for age at culling, risk of culling was significantly higher for infertility (HR = 1.70), left displaced abomasum (HR = 3.15), and peripartum problems (HR = 2.10) compared with voluntary culling. In conclusion, farmers tend to keep infertile cows for longer period from calving to culling while infertile cows are generally culled at younger age. Also, early culling appeared to have a high proportion of culls in the studied herds.