Edaphic fauna in soil profile after three decades of different soil management and cover crops in a subtropical region.

This research evaluated the effects of long-term (30 years) winter cover crops under conventional farming system and no-tillage system on edaphic fauna in a Rhodic Hapludox soil, from Paraná State, Brazil. We used three winter cover crops (black oat, hairy vetch and fallow), and as a reference a fragment of natural forest. Soil monoliths were collected at two times, one during the flowering of maize (April 2013) and the other during the flowering of soybean (January 2014). The extraction of the monoliths was carried out in three layers in the soil profile (0-10, 10-20 and 20-30 cm). Seventeen taxonomic groups were sampled. The density of the edaphic fauna is inversely related to soil depth. The winter crops associated with the no-tillage system in long-term resulted in fauna densities similar to the natural environment, with a higher density (density increase of 2.2x) at a depth of 10-20 cm in areas with black oat. At 0-10 cm depth, black oat and vetch under no-tillage systems resulted in an increase of 62% and 69% (April 2013) and 46 and 44% (January 2014), respectively, in the density of soil fauna, when compared to the same winter crops in conventional farming system.

Lanostane-type triterpenes from the fungal endophyte Scleroderma UFSMSc1 (Persoon) Fries.

Two lanostane triterpenoids (sclerodols A and B) were isolated from the culture of the Eucalyptus grandis derived from the endophyte Scleroderma UFSM Sc1(Persoon) Fries together with three known compounds: one related triterpenoid lanosta-8,23-dien-3ß,25-diol, the disaccharide α,ß-trehalose, and the sugar alcohol mannitol. Their structures were elucidated on the basis of 2D NMR, HRME, and single-crystal X-ray diffraction data. The methanol crude extract and the isolated lanostane triterpenoids showed promising anticandidal activities.

Molecular and morphological analyses confirm Rhizopogon verii as a widely distributed ectomycorrhizal false truffle in Europe, and its presence in South America.

The genus Rhizopogon includes species with hypogeous or subepigeus habit, forming ectomycorrhizae with naturally occurring or planted pines (Pinaceae). Species of the genus Rhizopogon can be distinguished easily from the other hypogeous basidiomycetes by their lacunose gleba without columella and their smooth elliptical spores; however, the limit between species is not always easy to establish. Rhizopogon luteolus, the type species of the genus, has been considered one of the species that are more abundant in Europe, as well as it has been cited in pine plantation of North and South America, different parts of Africa, Australia, and New Zealand. However, in this study, based on molecular analyses of the ITS nuclear ribosomal DNA (nrDNA) sequences (19 new sequences; 37 sequences from GenBank/UNITE, including those from type specimens), we prove that many GenBank sequences under R. luteolus were misidentified and correspond to Rhizopogon verii, a species described from Tunisia. Also, we confirm that basidiomes and ectomycorrhizae recently collected in Germany under Pinus sylvestris, as well as specimens from South of Brazil under Pinus taeda belong to R. verii. Thanks to the numerous ectomycorrhizal tips collected in Germany, a complete description of R. verii/P. sylvestris ectomycorrhiza is provided. Moreover, since in this paper the presence of R. verii in South America is here reported for the first time, a short description of basidiomes collected in Brazil, compared with collections located in different European herbaria, is included.