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1.
Int J Parasitol Parasites Wildl ; 21: 59-68, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37182113

RESUMEN

Tachaea chinensis is an ectoparasite commonly found on diverse ecologically and commercially valuable freshwater shrimps and prawns. Previous studies on this parasite have focused on its distribution and taxonomical identification, while its host preference and/or the potential predation in this host-parasite interaction remained poorly understood. In this study, we investigate the host preference and potential predation of the isopod T. chinensis using manipulative choice and predation experiments under laboratory settings. The preference toward a broad range of host decapods in single-host treatments, indicates a low host specificity, which ultimately aids in the survival of this parasite in the natural environment. Tachaea chinensis responded well to the shrimp Palaemon paucidens when presented with uncommon host species in all three treatments. In host-parasite predation treatments, all the tested P. paucidens shrimp, the prawn Macrobrachium nipponense, and the crayfish Procambarus clarkii were able to consume the isopod-especially the invasive crayfish P. clarkii, which consumed a greater percentage in a considerably shorter time frame (Fisher's exact test, P < 0.01). This study demonstrated for the first time the ability of larger freshwater decapods to prey upon T. chinensis. Despite the large difference in the maximum attainable size of those freshwater species, a high predation pressure by the invasive crayfish on the isopod is anticipated, if they are present in the same environment.

2.
Sci Rep ; 11(1): 23715, 2021 12 09.
Artículo en Inglés | MEDLINE | ID: mdl-34887468

RESUMEN

Cruciferous vegetables are rich sources of glucosinolates (GSLs). GSLs are degraded into isothiocyanates, which are potent anticarcinogens, by human gut bacteria. However, the mechanisms and enzymes involved in gut bacteria-mediated GSL metabolism are currently unclear. This study aimed to elucidate the enzymes involved in GSL metabolism in lactic acid bacteria, a type of gut bacteria. Companilactobacillus farciminis KB1089 was selected as a lactic acid bacteria strain model that metabolizes sinigrin, which is a GSL, into allylisothiocyanate. The sinigrin-metabolizing activity of this strain is induced under glucose-absent and sinigrin-present conditions. A quantitative comparative proteomic analysis was conducted and a total of 20 proteins that were specifically expressed in the induced cells were identified. Three candidate proteins, ß-glucoside-specific IIB, IIC, IIA phosphotransferase system (PTS) components (CfPttS), 6-phospho-ß-glucosidase (CfPbgS) and a hypothetical protein (CfNukS), were suspected to be involved in sinigrin-metabolism and were thus investigated further. We hypothesize a pathway for sinigrin degradation, wherein sinigrin is taken up and phosphorylated by CfPttS, and subsequently, the phosphorylated entity is degraded by CfPbgS. As expression of both pttS and pbgS genes clearly gave Escherichia coli host strain sinigrin converting activity, these genes were suggested to be responsible for sinigrin degradation. Furthermore, heterologous expression analysis using Lactococcus lactis suggested that CfPttS was important for sinigrin degradation and CfPbgS degraded phosphorylated sinigrin.


Asunto(s)
Metabolismo de los Hidratos de Carbono , Regulación Bacteriana de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Glucosinolatos/metabolismo , Lactobacillus/enzimología , Lactobacillus/genética , Familia de Multigenes , Proteoma , Proteómica/métodos , Metabolismo Secundario
3.
Diabetes ; 66(5): 1222-1236, 2017 05.
Artículo en Inglés | MEDLINE | ID: mdl-28209760

RESUMEN

Low-grade sustained inflammation links obesity to insulin resistance and nonalcoholic fatty liver disease (NAFLD). However, therapeutic approaches to improve systemic energy balance and chronic inflammation in obesity are limited. Pharmacological activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) alleviates obesity and insulin resistance in mice; however, Nrf2 inducers are not clinically available owing to safety concerns. Thus, we examined whether dietary glucoraphanin, a stable precursor of the Nrf2 inducer sulforaphane, ameliorates systemic energy balance, chronic inflammation, insulin resistance, and NAFLD in high-fat diet (HFD)-fed mice. Glucoraphanin supplementation attenuated weight gain, decreased hepatic steatosis, and improved glucose tolerance and insulin sensitivity in HFD-fed wild-type mice but not in HFD-fed Nrf2 knockout mice. Compared with vehicle-treated controls, glucoraphanin-treated HFD-fed mice had lower plasma lipopolysaccharide levels and decreased relative abundance of the gram-negative bacteria family Desulfovibrionaceae in their gut microbiomes. In HFD-fed mice, glucoraphanin increased energy expenditure and the protein expression of uncoupling protein 1 (Ucp1) in inguinal and epididymal adipose depots. Additionally, in this group, glucoraphanin attenuated hepatic lipogenic gene expression, lipid peroxidation, classically activated M1-like macrophage accumulation, and inflammatory signaling pathways. By promoting fat browning, limiting metabolic endotoxemia-related chronic inflammation, and modulating redox stress, glucoraphanin may mitigate obesity, insulin resistance, and NAFLD.


Asunto(s)
Tejido Adiposo Pardo/efectos de los fármacos , Tejido Adiposo Blanco/efectos de los fármacos , Endotoxemia , Glucosinolatos/farmacología , Imidoésteres/farmacología , Resistencia a la Insulina , Hígado/efectos de los fármacos , Enfermedad del Hígado Graso no Alcohólico/metabolismo , Obesidad/metabolismo , Tejido Adiposo Pardo/metabolismo , Tejido Adiposo Blanco/metabolismo , Animales , Desulfovibrio , Dieta Alta en Grasa , Metabolismo Energético/efectos de los fármacos , Microbioma Gastrointestinal/efectos de los fármacos , Inflamación , Peroxidación de Lípido/efectos de los fármacos , Lipopolisacáridos/sangre , Hígado/metabolismo , Macrófagos/efectos de los fármacos , Ratones , Ratones Noqueados , Factor 2 Relacionado con NF-E2/genética , Oximas , Sulfóxidos , Proteína Desacopladora 1/efectos de los fármacos , Proteína Desacopladora 1/metabolismo
4.
World J Gastroenterol ; 21(43): 12457-67, 2015 Nov 21.
Artículo en Inglés | MEDLINE | ID: mdl-26604653

RESUMEN

AIM: To evaluate effects of dietary supplementation of sulforaphane (SF)-rich broccoli sprout (BS) extract on hepatic abnormalities in Japanese male participants. METHODS: In a randomized, placebo-controlled, double blind trial, male participants with fatty liver received either BS capsules containing glucoraphanin [GR; a precursor of SF (n = 24)] or placebo (n = 28) for 2 mo. Liver function markers, serum levels of aspartate and alanine aminotransferases (AST and ALT, respectively) and γ-glutamyl transpeptidase (γ-GTP) and an oxidative stress marker, urinary levels of 8-hydroxydeoxyguanosine (8-OHdG), were measured and compared in participants before and after the trial period. In an animal model, chronic liver failure was induced in Sprague-Dawley rats by successive intraperitoneal injection with N-nitrosodimethylamine (NDMA) for 4 wk. Concomitantly, rats received AIN-76 diets supplemented with or without BS extract. Thereafter, rats were sacrificed, and their sera and livers were collected to measure serum liver function markers and hepatic levels of thiobarbituric acid reactive substances (TBARS) levels and hepatic glutathione S-transferase (GST) activity, a prototypical phase 2 antioxidant enzyme. RESULTS: Dietary supplementation with BS extract containing SF precursor GR for 2 mo significantly decreased serum levels of liver function markers, ALT [median (interquartile range), before: 54.0 (34.5-79.0) vs after supplementation: 48.5 (33.3-65.3) IU/L, P < 0.05] and γ-GTP [before: 51.5 (40.8-91.3) vs after: 50.0 (37.8-85.3) IU/L, P < 0.05], as well as the alkali phosphatase activity. Placebo showed no significant effects on the markers. The urinary level of 8-OHdG, an established oxidative stress marker, was significantly reduced in participants who had received BS capsules but not the placebo [before: 6.66 (5.51-9.03) vs after: 5.49 (4.89-6.66) ng/mg-creatinine, P < 0.05]. The reduction of urinary 8-OHdG was significantly correlated with decreased levels of both ALT and γ-GTP [∆8-OHdG and ∆ALT: Spearman r (r) 0.514 and P = 0.012, ∆8-OHdG and ∆γ-GTP: r = 0.496 and P = 0.016]. Intake of BS extract prevented NDMA-induced chronic liver failure in rats, which was attributable to the suppression of the increase in TBARS through induction of hepatic phase 2 antioxidant enzymes including hepatic GST (86.6 ± 95.2 vs 107.8 ± 7.7 IU/g, P < 0.01). CONCLUSION: Dietary supplementation with BS extract containing the SF precursor GR is likely to be highly effective in improving liver function through reduction of oxidative stress.


Asunto(s)
Antioxidantes/uso terapéutico , Brassica/química , Suplementos Dietéticos , Hígado Graso/tratamiento farmacológico , Isotiocianatos/uso terapéutico , Extractos Vegetales/uso terapéutico , Adulto , Animales , Antioxidantes/efectos adversos , Biomarcadores/sangre , Suplementos Dietéticos/efectos adversos , Modelos Animales de Enfermedad , Método Doble Ciego , Hígado Graso/sangre , Hígado Graso/diagnóstico , Humanos , Isotiocianatos/efectos adversos , Isotiocianatos/aislamiento & purificación , Japón , Pruebas de Función Hepática , Masculino , Persona de Mediana Edad , Estrés Oxidativo/efectos de los fármacos , Fitoterapia , Extractos Vegetales/efectos adversos , Extractos Vegetales/aislamiento & purificación , Plantas Medicinales , Ratas Sprague-Dawley , Plantones , Sulfóxidos , Factores de Tiempo , Resultado del Tratamiento
5.
PLoS One ; 7(6): e38029, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22701595

RESUMEN

The P/Q-type voltage-dependent calcium channels (VDCCs) are essential for synaptic transmission at adult mammalian neuromuscular junctions (NMJs); however, the subsynaptic location of VDCCs relative to active zones in rodent NMJs, and the functional modification of VDCCs by the interaction with active zone protein Bassoon remain unknown. Here, we show that P/Q-type VDCCs distribute in a punctate pattern within the NMJ presynaptic terminals and align in three dimensions with Bassoon. This distribution pattern of P/Q-type VDCCs and Bassoon in NMJs is consistent with our previous study demonstrating the binding of VDCCs and Bassoon. In addition, we now show that the interaction between P/Q-type VDCCs and Bassoon significantly suppressed the inactivation property of P/Q-type VDCCs, suggesting that the Ca(2+) influx may be augmented by Bassoon for efficient synaptic transmission at NMJs. However, presynaptic Bassoon level was significantly attenuated in aged rat NMJs, which suggests an attenuation of VDCC function due to a lack of this interaction between VDCC and Bassoon. Importantly, the decreased Bassoon level in aged NMJs was ameliorated by isometric strength training of muscles for two months. The training increased Bassoon immunoreactivity in NMJs without affecting synapse size. These results demonstrated that the P/Q-type VDCCs preferentially accumulate at NMJ active zones and play essential role in synaptic transmission in conjunction with the active zone protein Bassoon. This molecular mechanism becomes impaired by aging, which suggests altered synaptic function in aged NMJs. However, Bassoon level in aged NMJs can be improved by muscle exercise.


Asunto(s)
Envejecimiento/fisiología , Canales de Calcio Tipo P/metabolismo , Canales de Calcio Tipo Q/metabolismo , Regulación de la Expresión Génica/fisiología , Proteínas del Tejido Nervioso/metabolismo , Unión Neuromuscular/fisiología , Condicionamiento Físico Animal/fisiología , Transmisión Sináptica/fisiología , Envejecimiento/metabolismo , Animales , Calcio/metabolismo , Canales de Calcio Tipo N , Canales de Calcio Tipo P/genética , Canales de Calcio Tipo Q/genética , Línea Celular , Cricetinae , Procesamiento de Imagen Asistido por Computador , Inmunohistoquímica , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Microscopía Confocal , Técnicas de Placa-Clamp , Terminales Presinápticos/metabolismo , Ratas , Ratas Sprague-Dawley
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