RESUMEN
DNA sequences encoding the GroES and GroEL proteins of Orientia tsutsugamushi were amplified by the PCR and sequenced. Pairwise alignment of full-length groES and groEL gene sequences indicated high sequence similarity (90.4-100% and 90.3-100%) in O. tsutsugamushi, suggesting that these genes are good candidates for the molecular diagnosis and phylogenetic analysis of scrub typhus. Comparisons of the 56-kD type-specific antigen (TSA) protein gene and the groES and groEL genes showed that genotypes based on the 56-kD TSA gene were not related to a cluster containing the groES and groEL genes in a dendrogram, suggesting that a gene rearrangement may be associated with homologous recombination in mites.
Asunto(s)
Chaperonina 10/genética , Chaperonina 60/genética , Orientia tsutsugamushi/genética , Tifus por Ácaros/microbiología , Animales , Proteínas Bacterianas/genética , Secuencia de Bases , ADN Bacteriano/química , ADN Bacteriano/genética , Genotipo , Orientia tsutsugamushi/clasificación , Orientia tsutsugamushi/aislamiento & purificación , Filogenia , Reacción en Cadena de la Polimerasa , Tifus por Ácaros/diagnóstico , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
OBJECTIVE: To assess the prevalence including asymptomatic infection, infection risk of exposure to patients, and effectiveness of personal protective equipment (PPE) among health care workers (HCWs) during the first pandemic (H1N1) 2009 (pH1N1) outbreak in Kobe, Japan in May 2009. METHODS: A cross-sectional seroepidemiological study was conducted on 268 HCWs in the two hospitals in Kobe to which all pH1N1 inpatients were directed. Participating HCWs completed a self-administrated questionnaire and provided a single serum sample which was analyzed using a hemagglutination-inhibition (HI) antibody test. RESULTS: Of 268 subjects, 14 (5.2%) were found to have positive antibodies to the pH1N1 by HI assay; only 1 reported a febrile episode. Among the 14 seropositive cases, 8 received chemoprophylaxis. 162 HCWs (60.4%) had been exposed to patients. The seropositive rate (SPR) for pH1N1 of the exposed group was higher than that of the unexposed group, however not statistically significant (6.8% vs. 3.1%, p = 0.197). There were no statistically significant differences in SPR for any PPE. CONCLUSION: The SPR for pH1N1 in the exposed group was higher than that of the unexposed group in HCWs; however, most of these individuals were asymptomatic. There was no statistically significant association between PPE implementation and pH1N1 seropositivity.
Asunto(s)
Anticuerpos Antivirales/sangre , Personal de Salud , Subtipo H1N1 del Virus de la Influenza A/inmunología , Gripe Humana/epidemiología , Pandemias , Adulto , Anciano , Enfermedades Asintomáticas/epidemiología , Estudios Transversales , Femenino , Pruebas de Inhibición de Hemaglutinación , Hospitales Generales , Humanos , Control de Infecciones/métodos , Gripe Humana/inmunología , Gripe Humana/prevención & control , Gripe Humana/virología , Japón/epidemiología , Masculino , Persona de Mediana Edad , Ropa de Protección/estadística & datos numéricos , Estudios Seroepidemiológicos , Encuestas y CuestionariosRESUMEN
We evaluated the antiviral activity of a chlorine dioxide gas solution (CD) and sodium hypochlorite (SH) against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. CD at concentrations ranging from 1 to 100 ppm produced potent antiviral activity, inactivating >or= 99.9% of the viruses with a 15 sec treatment for sensitization. The antiviral activity of CD was approximately 10 times higher than that of SH.
Asunto(s)
Antivirales/farmacología , Compuestos de Cloro/farmacología , Óxidos/farmacología , Hipoclorito de Sodio/farmacología , Adenovirus Caninos/efectos de los fármacos , Adenovirus Humanos/efectos de los fármacos , Calicivirus Felino/efectos de los fármacos , Virus del Moquillo Canino/efectos de los fármacos , Herpesvirus Humano 1/efectos de los fármacos , Virus del Sarampión/efectos de los fármacos , Orthomyxoviridae/efectos de los fármacos , Parvovirus Canino/efectos de los fármacosRESUMEN
We conducted an anti-varicella-zoster virus antibody seroepidemiologic survey comparing the pre- and postvaccine eras of 1977-1981 and 2001-2005. For each period, 828 samples were measured by enzyme immunoassay test and compared. The differences from 1-year-old to high-school aged children were statistically significant. The introductions of optional varicella immunization and lifestyle changes for children were considered influencing factors.
Asunto(s)
Anticuerpos Antivirales/sangre , Varicela/epidemiología , Herpesvirus Humano 3/inmunología , Adolescente , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Vacuna contra la Varicela/inmunología , Niño , Preescolar , Femenino , Humanos , Técnicas para Inmunoenzimas , Lactante , Recién Nacido , Japón/epidemiología , Masculino , Persona de Mediana Edad , Estudios Seroepidemiológicos , Adulto JovenRESUMEN
Using the adverse events monitoring system of Japan, we observed diarrhea cases in approximately 10% of patients who received oral poliovirus vaccine (OPV). This study was conducted to investigate whether diarrhea among children aged 0 to 1 is caused by OPV or by other factors such contact at the doctor's office and/or with others outside the home. We conducted a survey of the health of children after regular health check-ups and after the administration of the OPV. The data from the health check-ups were used as a control for the OPV case group. We compared the first-OPV dose vaccination group as well as the second-OPV dose vaccination group to the health check-up group. For cases of diarrhea, the odds ratio of the OPV group to the health check-up group was 1.776. Our findings strongly suggest that post-OPV cases of mild diarrhea are closely related to the administration of the OPV.
