Fluorescent microscopy evaluation of diode laser effect on the penetration depth of turmeric (Curcuma longa) extract cream on skin tissues of Wistar rats.

The study investigates the effect of diode laser exposure on curcumin's skin penetration, using turmeric extraction as a light-sensitive chemical and various laser light sources. It uses an in vivo skin analysis method on Wistar strain mice. The lasers are utilized at wavelengths of 403 nm, 523 nm, 661 nm, and 979 nm. The energy densities of the lasers are 20.566 J/cm2, 20.572 J/cm2, 21.162 J/cm2, and 21.298 J/cm2, which are comparable to one another. The experimental animals were divided into three groups: base cream (BC), turmeric extract cream (TEC), and the combination laser (L), BC, and TEC treatment group. Combination light source (LS) with cream (C) was performed with 8 combinations namely 523 nm ((L1 + BC) and (L1 + TEC)), 661 nm ((L2 + BC) and (L2 + TEC)), 403 nm ((L3 + BC) and (L3 + TEC)), and 979 nm ((L4 + BC) and (L4 + TEC)). The study involved applying four laser types to cream-covered and turmeric extract-coated rat skin, with samples scored for analysis. The study found that both base cream and curcumin cream had consistent pH values of 7-8, within the skin's range, and curcumin extract cream had lower viscosity. The results of the statistical analysis of Kruskal-Wallis showed a significant value (p < 0.05), which means that there are at least two different laser treatments. The results of the post hoc analysis with Mann-Whitney showed that there was no significant difference in the LS treatment with the addition of BC or TEC when compared to the BC or TEC treatment alone (p > 0.05), while the treatment using BC and TEC showed a significant difference (p < 0.05). Laser treatment affects the penetration of the turmeric extract cream into the rat skin tissue.

Antibacterial effect of red laser-activated silver nanoparticles synthesized with grape seed extract against Staphylococcus aureus and Escherichia coli.

Living organisms, particularly humans, frequently encounter microorganisms such as bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used in biomedical devices because of their antibacterial and antiviral properties. The study evaluates the efficacy of red laser and silver nanoparticles from grape seed extract (AgNPs-GSE) in reducing Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, which cause infections. The sample comprised three groups: a control group without laser irradiation (T0), Escherichia coli samples (A1 and A2) irradiated with a 405-nm diode laser at different times and concentrations of silver nanoparticles, and Staphylococcus aureus samples (A3 and A4) illuminated with a 405-nm diode laser at different times and concentrations. Bacteria in groups A2 and A4 were treated with a photosensitizer (PS) made from grape seed extracts, incubated for 10 min, and then irradiated for 90, 120, 150, and 180 s. The samples were cultured on TSA media, set at 37 °C, counted using a Quebec colony counter, and analyzed using ANOVA and Tukey tests with a significance level of p < 0.05. The study illustrated that the combination of 10 µl of AgNPs-GSE, exposure to a red laser at 405 nm, and an energy density of 3.44 J/cm2 effectively photoinactivated both Escherichia coli and Staphylococcus aureus bacteria. For Escherichia coli bacteria irradiated for 180 s with concentrations of 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, bacterial viability decreased by 64.50%, 70.74%, and 79.53%, respectively. Similarly, Staphylococcus aureus bacteria, subjected to irradiation for 180 s with concentrations of 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, demonstrated reductions in bacterial viability by 70.23%, 73.47%, and 85.04%, respectively. The findings from the present study indicate that at an energy density of 3.44 J/cm2, it was possible to inactivate Escherichia coli by 79.53% and Staphylococcus aureus by 85.04%.

Photobiomodulation effect of infra-red laser on the level of gonad maturity in the Simese Catfish (Pangasianodon hypophthalmus).

The purpose of this study is to determine how photo biomodulation therapy utilizing infrared diode laser irradiation (975.2 nm) affects the gonadal maturity level (GML) of male Siamese catfish (Pan-gasianodon hypothalamus). The interest in applying laser therapy in medicine and dentistry has remarkably increased in the last decade. Different types of lasers are available, and their usage is well-defined by different parameters, such as wavelength, energy density, power output, duration of radiation, power density and radiation mode. Infrared diode laser irradiation is used at the reproductive point (governor's vessel), situated 2/3 of the way between the anus and the pectoral fin. This study examined the metrics GML, gonads somatic index, and hepatosomatic index. The treatments were Control+ (ovaprim), Control- (without the treatment), P1 (0.2 J/cm2), P2 (0.4 J/cm2), P3 (0.6 J/cm2), and P4 (0.8 J/cm2). Therapy with infrared diode laser irradiation can modify gonad maturity (GML), gonadosomal index, and hepatosomatic index in male Siamese catfish. The photobiomodulation effect of an infrared laser stimulated the gonadal maturation of Siamese catfish. This is based on the values of wavelength (nm), power (mW), beam area (cm2), time (s), radiation mode (rad) and energy dose (J/cm2) in Control- (no treatment), control+ (ovaprim), P1, P2, P3, and P4. The increase in the observed parameter values is due to the vitellogenesis process. The fish gonads at the GML IV had the highest GML at P2 (dose 0.4 J/cm2), with a GSI value of 1.02% and an HSI value of 1.46%. According to the study's findings, photo biomodulation therapy with infrared diode laser exposure at a dose of 0.4 J/cm2 is the best way to increase the gonad maturity of male Siamese catfish.

Acceleration of gonadal maturity of female striped catfish, Pangasianodon hypophthalmus (Pisces, Pangasiidae) by irradiation to low-power soft-laser.

This study was aimed to investigate the effects of red photodiode soft-laser irradiation on gonadal maturity and egg quality of female striped catfish, Pangasianodon hypophthalmus. Four female striped catfish (weighing 700-800 g) at stage I of gonadal maturation (for all treatments) were exposed four times (once a week) to different doses (0.2, 0.4, 0.6, and 0.8 J/cm2) of red photodiode soft-laser in the reproductive acupoint. Doses of 500 IU and 0.5 mL per kg body weight of human chorionic gonadotropin (hCG) and Ovaprim™, respectively were injected intramuscularly at week 4 (positive control), and no treatment (negative control). Finally, gonadal maturation stage (GMS), gonadosomatic index (GSI), hepatosomatic index (HSI), fecundity, and egg diameter were measured at week 5. The results showed that red photodiode soft-laser irradiation of 0.4 J/cm2 dose accelerated GMS IV at early week 3 (100 %) and increases GSI (3.42 %), HSI (1.26 %), fecundity (67,665 eggs/body weight), and egg diameter (1.01 mm) compared to the other treatments. Treatments of 0.2, 0.6, and 0.8 J/cm2 doses, and positive and negative controls only reached GMS III (75 %), III (25 %), I (100 %), II (25 %), and I (100 %), respectively. GSI values were 2.20 %, 1.47 %, 0.93 %, 0.44 %, and 0.42 %, respectively and HSI values were 1.07 %, 0.85 %, 0.7 7%, 0.66 %, and 0.53 %, respectively. Females irradiated with doses of 0.2 and 0.6 J/cm2 had fecundities of 38,822 and 29,530 eggs/body weight, respectively, and egg diameters of 0.88 and 0.29 mm, respectively. These results suggest that a red photodiode soft-laser irradiation accelerates gonadal maturity and improves egg quality in female striped catfish.

Effectiveness of ozone-laser photodynamic combination therapy for healing wounds infected with methicillin-resistant Staphylococcus aureus in mice.

Background and Aim: According to 2013 data from the Ministry of Health of the Republic of Indonesia, there were 8.2% more wounds than typical in Indonesia; 25.4% were open wounds, 70.9% were abrasions and bruises, and 23.2% were lacerations. A wound is defined as damage or loss of body tissue. This study aimed to determine the effectiveness of wound healing using red-laser therapy (650 nm, 3.5 J/cm2), blue-laser therapy (405 nm, 3.5 J/cm2), ozone therapy, red-laser therapy (650 nm, 3.5 J/cm2) with ozone, and blue-laser therapy (405 nm, 3.5 J/cm2) with ozone. Materials and Methods: One hundred and twelve mice were given incision wounds and infected with methicillin-resistant Staphylococcus aureus (MRSA). The study used a factorial design with two factors: The type of therapy (n = 7) and irradiation time (days 1, 2, 4, and 6). The mice were divided into seven therapy groups: Control group with NaCl, control with Sofra-tulle® treatment, red-laser therapy (650 nm, 3.5 J/cm2), blue-laser therapy (405 nm, 3.5 J/cm2), ozone therapy, red-laser therapy (650 nm, 3.5 J/cm2) with ozone, and blue-laser therapy (405 nm, 3.5 J/cm2) with ozone. This therapy was performed using irradiation perpendicular to the wound area. The photosensitizer used was curcumin 10 mg/mL, which was applied to the wound area before exposure to a laser and ozone. The ozone concentration was 0.011 mg/L with a flow time of 80 s. The test parameters were the number of collagens, bacterial colonies, lymphocytes, monocytes, and wound length measurement to determine their acceleration effects on wound healing. Data were analyzed by a two-way (factorial) analysis of variance test. Results: Acceleration of wound healing was significantly different between treatments with a laser or a laser-ozone combination and treatment using 95% sodium chloride (NaCl) and Sofra-tulle®. On day 6, the blue-laser with ozone treatment group had efficiently increased the number of bacteria and reduced the wound length, and the red-laser treatment with ozone increased the amount of collagen. In addition, the red-laser also reduced the number of lymphocytes and monocytes, which can have an impact on accelerating wound healing. Blue-laser therapy was very effective for increasing the number of epithelia. Conclusion: The blue- and red-laser combined with ozone treatments effectively accelerated the healing of incisional wounds infected with MRSA bacteria.

Design and Application of Near Infrared LED and Solenoid Magnetic Field Instrument to Inactivate Pathogenic Bacteria.

PURPOSE: This study aims to evaluate the efficiency of infrared LEDs with a magnetic solenoid field in lowering the quantity of gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacteria, as well as the best exposure period and energy dose for inactivating these bacteria. METHOD: Research has been performed on a photodynamic therapy technique called photodynamic inactivation (PDI), which combines infrared LED light with a wavelength range of 951-952 nm and a solenoid magnetic field with a strength of 0-6 mT. The two, taken together, can potentially harm the target structure biologically. Infrared LED light and an AC-generated solenoid magnetic field are both applied to bacteria to measure the reduction in viability. Three different treatments infrared LED, solenoid magnetic field, and an amalgam of infrared LED and solenoid magnetic field, were used in this study. A factorial statistical ANOVA analysis was utilized in this investigation. RESULTS: The maximum bacterial production was produced by irradiating a surface for 60 min at a dosage of 0.593 J/cm2, according to the data. The combined use of infrared LEDs and a magnetic field solenoid resulted in the highest percentage of fatalities for Staphylococcus aureus, which was 94.43 s. The highest percentage of inactivation for Escherichia coli occurred in the combination treatment of infrared LEDs and a magnetic field solenoid, namely, 72.47 ± 5.06%. In contrast, S. aureus occurred in the combined treatment of infrared LEDs and a magnetic field solenoid, 94.43 ± 6.63 percent. CONCLUSION: Staphylococcus aureus and Escherichia coli germs are inactivated using infrared illumination and the best solenoid magnetic fields. This is evidenced by the rise in the proportion of bacteria that died in treatment group III, which used a magnetic solenoid field and infrared LEDs to deliver a dosage of 0.593 J/cm2 over 60 min. According to the research findings, the magnetic field of the solenoid and the infrared LED field significantly impact the gram-positive bacteria S. aureus and the gram-negative bacteria E. coli.

Effect of 650 nm laser photobiomodulation therapy on the HT-7 (shenmen) acupoint in the Mus musculus model of Parkinson's disease.

Background: Parkinson's disease is one of the neurodegenerative conditions that impacts 1-2% of the world's population. The only effective therapy for this condition today is to restore the biochemical function of the diseased dopamine neurons by giving them Levodopa or L-3,4-dihydroxyphenylalanine (l-DOPA). The risk of progenitor stem cells, though, is the growth of teratomas or the uncontrolled growth of cells. As a result, an alternative or additional method is needed, such as photobiomodulation therapy using a laser diode. In this research, male mice (Mus musculus), which were used as models for Parkinson's disease in an in vivo paraquat study, to determine the optimal dose of photobiomodulation therapy and a laser diode was used as a treatment. Methods: The three sample groups are Group P-L- (control group, induced by 0.9% NaCl), Group P + L- (only caused by paraquat), and Group P + L+. (Treatment group, treated by paraquat and photobiomodulation therapy with a laser diode). Photobiomodulation treatment doses of 0.14 J, 0.29 J, 0.37 J, 0.76 J, 1.14 J, and 1.52 J were used in the P+L+ subgroups (6 groups). The laser diode generated a continuous wave with a wavelength of 658 nm, a beam spot of 2.10 mm, and an output power of 15.42 mW. After treatment, the histopathology results of each sample were inspected under a microscope. Result: In Parkinson's disease-affected mice, paraquat has been shown to reduce the number of neurons. According to the results of the histopathological examination, photobiomodulation therapy using a laser diode (P + L+) on the HT-7 (Shenmen) may raise the quantity of neurons and the proportion of healthy cells in the mouse brain. Conclusion: The effective radiated energy of the photobiomodulation therapy using laser diode treatment on the muscle musculus cell model of Parkinson's disease is 0.76 J.

Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus.

Introduction: Living organisms, particularly humans, frequently encounter microorganisms like bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used in biomedical devices due to their antibacterial, antifungal, and antiviral properties. The study evaluates the efficacy of blue laser and silver nanoparticles from grape seed extract (AgNPs-GSE) in reducing gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria causing infections. Methods: The sample consisted of four groups: a control without laser irradiation (T0), E. coli samples (A1 and A2) irradiated with a 405 nm diode laser at different times and concentrations of silver nanoparticles, and S. aureus samples (A3 and A4) irradiated with a 405 nm diode laser at different times and concentrations. Bacteria in groups A2 and A4 were treated with a photosensitizer (PS) made from grape seed extracts, incubated for 10 minutes, and then irradiated for 90, 120, 150, and 180 seconds. The samples were cultured on Tryptic Soy Agar (TSA) media, incubated at 37 °C, counted by using a Quebec colony counter, and analyzed using ANOVA and Tukey tests with a significance level of P<0.05. Results: The study found that 10 µl of AgNPs-GSE, when combined with exposure to a blue laser at 405 nm and a dose of 3.44 J/cm2, can effectively photoinactivate E. coli and S. aureus bacteria. The addition of AgNPs-GSE to E. coli bacteria led to a significant reduction in their viability, with a reduction of 73.93%, 80.96%, and 83.80%, respectively. Similarly, when S. aureus bacteria were irradiated for 180 seconds by adding 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, bacterial viability was reduced by 70.87%, 78.04%, and 87.01%, respectively. Conclusion: The findings from the present study indicate that at an energy density of 3.44 J/cm2, it was possible to inactivate E. coli by 83.80% and S. aureus by 87.01%.

Gas Array Sensors based on Electronic Nose for Detection of Tuna (Euthynnus Affinis) Contaminated by Pseudomonas Aeruginosa.

Background: Fish is a food ingredient that is consumed throughout the world. When fishes die, their freshness begins to decrease. The freshness of the fish can be determined by the aroma it produces. The purpose of this study is to monitor the odor of fish using a collection of gas sensors that can detect distinct odors. Methods: The sensor was tested with three kinds of samples, namely Pseudomonas aeruginosa, tuna, and tuna that was contaminated with P. aeruginosa bacteria. During the process of collecting sensor data, all samples were placed in a vacuum so that the gas or aroma produced was not contaminated with other aromas. Eight sensors were used which were designed and implemented in an electronic nose (E-nose) device that can withstand aroma. The data collection process was carried out for 48 h, with an interval of 6 h for each data collection. Data processing was performed by using the principal component analysis and support vector machine (SVM) methods to obtain a plot score visualization and classification and to determine the aroma pattern of the fish. Results: The results of this study indicate that the E-nose system is able to smell fish based on the hour with 95% of the cumulative variance of the main component in the classification test between fresh tuna and tuna fish contaminated with P. aeruginosa. Conclusion: The SVM classifier was able to classify the healthy and unhealthy fish with an accuracy of 99%. The sensors that provided the highest response are the TGS 825 and TGS 826 sensors.

An in-vivo study of photobiomodulation using 403 nm and 649 nm diode lasers for molar tooth extraction wound healing in wistar rats.

PURPOSE: This study aims to examine the effects of red 649 nm 4 J/cm2 and blue 403 nm 8 J/cm2 diode laser treatment for post-extraction wounded healing in rats through histopathological and immunohistochemical analysis. METHODS: Samples of 54 Wistar rats were divided into six groups: C- control group without treatment; C + wounded group without treatment; TB wound group with Povidone-iodine treatment; TD wounded group with doxycycline treatment; TLB wounded group with 403 nm diode laser treatment; and TLR wounded group with 649 nm diode laser treatment. Mandibular samples were observed for the number of lymphocytes and fibroblasts cells, new blood vessels formation, Interleukin 1ß, and Collagen 1α expression level. RESULTS: Based on the histopathological test results, red laser diode treatment significantly increased the number of lymphocyte, fibroblast cells and the formation of new blood vessels. Meanwhile, immunohistochemical tests showed an increase in the expression of the Colagen-1α protein which plays a role in the formation of collagen for new tissues formation after damage, as well as a decrease in Interleukin-1ß expression level. Blue laser is also able to show a positive effect on wound healing even though its penetration level into the tissue is lower compared to red laser. CONCLUSION: The red diode laser 649 nm has been shown to accelerate the process of proliferation in wound healing post molar extraction based on histopathological and immunohistochemical test results.

Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis.

BACKGROUND: Periodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues. The aim of the study is determining the combination photodynamic effect of diode laser 405 nm treatments and the administration of doxycycline 0.1% within 1, 3, 5, and 7 days on a Wistar rat model of periodontitis. METHODS: Samples were induced with Porphyromonas gingivalis ATCC33277 to allow periodontitis development and were treated with combination of doxycycline and laser diode, then statistical analysis was carried out (One-Way ANOVA test and the post-hoc Duncan test; Kruskal-Wallis test and Mann-Whitney follow-up test for non-parametric data). Samples were divided into five groups, laser exposure used was 405-nm diode laser with energy density of 8 J/cm2. The expression level of histomorphometric was calculated by measuring the number of macrophages, lymphocytes, fibroblasts and the distance between the CEJ-AV. RESULTS: The results showed that the combination treatment of doxycycline and laser exposure yielded immunomodulatory effects. The expression level of fibroblast and the distance between CEJ-AV bone showed that the combination of doxycycline and laser therapy exerted healing effect in rat models of periodontitis on day 5 and 7. CONCLUSION: The combination of doxycycline 0.1% and diode laser therapy provides a healing effect in rats models of periodontitis.

The combined effect of magnetic and electric fields using on/off infrared light on the blood sugar level and the diameter of Langerhans islets of diabetic mice.

BACKGROUND AND AIM: At present, diabetes is treated with oral antidiabetic medicines, such as sulfonylureas and thiazolidine, as well as insulin injection. However, these methods have several shortcomings. Therefore, alternatives for treating diabetes mellitus (DM) are needed. This study aims to determine the combined effect of magnetic and electric fields on blood sugar levels and the diameter of Langerhans islets of diabetic mice. MATERIALS AND METHODS: Induction of DM in mice was carried out by administering lard for 2 weeks and continued with an intraperitoneal injection of streptozotocin, dissolved in a 4.5 pH citrate buffer, and administered in a dose of 30 mg/kg bodyweight for 5 days. Treatments were used in combination with magnetic and electric fields using on/off infrared light. Blood samples were pipetted through the tip of mice's tails to establish the blood sugar level for each individual mouse. Histology preparation of the pancreas organ was affected using the histology standard as well as hematoxylin and eosin staining methods. Langerhans islet diameter data were analyzed using analysis of variance followed by Duncan's multiple range test. Data analysis was performed at ssssssss=0.05. RESULTS: The results showed that the combined treatment of permanent magnetic and unidirectional electric fields (PS) caused changes in blood sugar levels that were not significantly different from the normal control group. The PS treatment improved the diameter of the Langerhans islets but not to a significant degree compared to other treatments. CONCLUSION: The use of PS treatment is effective for reducing the blood sugar levels of diabetic mice and improving the diameter of their Langerhans islets.

The efficacy of photodynamic inactivation with laser diode on Staphylococcus aureus biofilm with various ages of biofilm.

Biofilms are able to cause microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effectiveness of photodynamic inactivation with blue diode laser to reduce Staphylococcus aureus biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups: Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm2. Biofilm reduction measurement method using ELISA test was performed to calculate OD595 value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynamic with 403 nm laser diode is effective to reduce the Staphylococcus aureus biofilm in the maturation phase.

The effects of laser diode treatment on liver dysfunction of Mus musculus due to carbofuran exposure: An in vivo study.

OBJECTIVE: The aim of this study is to determine the effect of laser diode as an alternative treatment on liver dysfunction (in vivo study) that is caused by carbofuran using male mice (Mus musculus) strain Balb/C. MATERIALS AND METHODS: The samples were divided into three groups, namely, Group C-L- (control group, no treatment), Group C+L- (only treated by carbofuran treatment), and Group C+L+ (treatment group, treated by carbofuran and laser-puncture) with five replications each. After being treated, each liver slice of samples was observed using microscope to get the histology result and then scored. RESULTS: Carbofuran contamination can lead to inflammation of cells and necrosis. The histology results and the scoring test showed that the liver cells repair with the energy dose of laser diode at 0.5 and 1.0 Joule. CONCLUSION: The optimum energy dose in this study was 1.0 Joule which had the closest score of inflammatory cells and necrosis to normal liver cells.

Review of Polymeric Materials in 4D Printing Biomedical Applications.

The purpose of 4D printing is to embed a product design into a deformable smart material using a traditional 3D printer. The 3D printed object can be assembled or transformed into intended designs by applying certain conditions or forms of stimulation such as temperature, pressure, humidity, pH, wind, or light. Simply put, 4D printing is a continuum of 3D printing technology that is now able to print objects which change over time. In previous studies, many smart materials were shown to have 4D printing characteristics. In this paper, we specifically review the current application, respective activation methods, characteristics, and future prospects of various polymeric materials in 4D printing, which are expected to contribute to the development of 4D printing polymeric materials and technology.

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll.

Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans.

Gas Sensor Array System Properties for Detecting Bacterial Biofilms.

BACKGROUND: Gas sensor array system is a device that mimics the work of how the nose smells using the gas sensors that could give response toward specific odors. It is used for characterizing the different blended gas that is suited with the biological working nose principle. Thus, it could be used to detect the dental and oral diseases. Periodontitis is one of the diseases caused by the damage on the teeth due to the chronic infection on the gingival structure marked with bacterial plaque and calculus. This study aims to develop an electric nose for odor detection application on the periodontal bacterial biofilm as early detection device for dental and oral disease. METHODS: This device is designed as a portable device to ease the data acquisition. The measured data were stored at a database system connected to a real-time computer. A gas array sensor system with six gas sensors (TGS 826, TGS 2602, TGS 2600, TGS 2611, TGS 2612, and TGS 2620) has been assembled for the early detection application for dental and oral disease excreted by the bacterial biofilm that caused dental and oral disease, including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans, and Enterococcus faecalis. RESULTS: TGS 826 and TGS 2602 sensor had the best response showed by the high ADC delta value. CONCLUSION: GS 826 and TGS 2602 sensor could be used as a candidate for early detection device for dental and oral disease.

COMPARISON OF ANTI BACTERIAL EFFICACY OF PHOTODYNAMIC THERAPY AND DOXYCYCLINE ON AGGREGATIBACTER ACTINOMYCETEMCOMITANS.

BACKGROUND: Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) is an anaerobic bacterium has been frequently associated with aggressive periodontitis. Photodynamic therapy (PDT) is a medical treatment to prevent infection progression that utilizes light to activate a photosensitizing agent. Doxycycline is an antibacterial having photosensitivity. This study aimed to evaluate potential doxycycline as an antibacterial and photosensitizer combine PDT against A.actinomycetemcomitans bacteria. MATERIAL AND METHODS: Samples were distributed to 4 groups as follow: (1) Groups A treated with a diode laser, (2) Group B treated with doxycycline 0,1% and laser, (3) Group C treated only with Doxycycline 0.1%, and (4) Group D no exposure doxycycline/laser. Data were analyzed by one-way ANOVA and Tukey's HSD test at 5% significance level. RESULTS: In this study, doxycycline 0.1% has the effect of reducing the bacterial viability of (59.60±3.26%). Whereas laser exposure 120 s combined with doxycycline produce the effect of bacterial viability reduction (88.50±2.83%) is not significantly different from the effect of laser 120 s exposure (88.79±2.60%). In clinical treatment, the application of laser exposure is generally at the duration of the exposure time of 30 s. The results of this study indicate that the duration of laser exposure 30 s shows a reduction in bacterial viability (44.91±1.69%) equal to the laser and with a combination of doxycycline and laser exposure (70.70±2.43%). So at low doses of laser exposure, doxycycline 0.1% combined with the laser results in the greatest reduction in bacterial viability, significantly different from laser exposure alone. CONCLUSION: at low doses of laser exposure (30s with energy 3.68 J/cm2), doxycycline 0.1% combined with the laser results in the greatest reduction in bacterial viability, significantly different with laser exposure alone.