RESUMEN
The results ofMTHFR gene C(677)-->T (rs1801133) polymorphism determined in 170 patients with ischemic atherothrombotic stroke (IATS) and 124 healthy subjects (control group) are presented in the paper. It has been shown that in patients with IATS, the frequencies of main homozygotes (CC), heterozygotes (CT) and minor homozygotes (TT) are 52.4, 35.9, 11.8% (in control--46.0, 48.4, 5.6%, P = 0.044 by chi2-test). TT homozygotes have a greater chance of developing IATS than carriers of main C-allele (CT + CC) (OR = 2.3, CI = 0.911-5.449, P = 0.049). In the representatives of the Ukrainian population there is a relationship between the frequency of MTHFR gene C(677)-->T polymorphism genotypes and the risk of IATS. This connection is manifested in male patients, in persons with normal blood pressure, and in people who do not have the habit of smoking. The sex of the patients, body mass index, blood pressure and smoking affect the level of the studied polymorphism association with stroke.
Asunto(s)
Isquemia Encefálica/genética , Infarto de la Arteria Cerebral Anterior/genética , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Polimorfismo de Nucleótido Simple , Adulto , Anciano , Anciano de 80 o más Años , Alelos , Índice de Masa Corporal , Isquemia Encefálica/enzimología , Isquemia Encefálica/etnología , Isquemia Encefálica/patología , Femenino , Frecuencia de los Genes , Heterocigoto , Homocigoto , Humanos , Infarto de la Arteria Cerebral Anterior/enzimología , Infarto de la Arteria Cerebral Anterior/etnología , Infarto de la Arteria Cerebral Anterior/patología , Masculino , Metilenotetrahidrofolato Reductasa (NADPH2)/metabolismo , Persona de Mediana Edad , Factores de Riesgo , Factores Sexuales , Fumar , Ucrania , Población BlancaRESUMEN
G-7A polymorphism (rs1800801) of matrix Gla protein (MGP) gene in 170 patients with ischemic atherothrombotic stroke (IATS) and in 124 persons of the control group was determined. It was shown that in patients with IATS the distribution of the major allele homozygotes, heterozygotes and minor allele homozygotes was 35.9; 48.8; 15.3% (in control--43.5; 50.0; 6.5%, P = 0.051 by chi2-test). Significant differences in the distribution of genotypes were revealed only in women (P = 0.022). Odds ratio for minor allele homozygotes (A/A) versus major allele carriers (G/A+G/G) was 2,618 (P = 0.023), while in women it was equal to 6,645 (P = 0.015). In patients with A/A genotype the values of blood coagulation parameters (prothrombin time) indicated increased predisposition to hypercoagulability. The results obtained suggest that the A/A-variant of MGP gene is associated with an increased risk of IATS in female persons of the Ukrainian population and may be related to blood hypercoagulability and thrombi formation.
Asunto(s)
Isquemia Encefálica/genética , Proteínas de Unión al Calcio/genética , Proteínas de la Matriz Extracelular/genética , Arteriosclerosis Intracraneal/genética , Polimorfismo de Nucleótido Simple , Accidente Cerebrovascular/genética , Trombofilia/genética , Adulto , Anciano , Anciano de 80 o más Años , Alelos , Isquemia Encefálica/complicaciones , Isquemia Encefálica/patología , Femenino , Frecuencia de los Genes , Genotipo , Heterocigoto , Homocigoto , Humanos , Arteriosclerosis Intracraneal/complicaciones , Arteriosclerosis Intracraneal/patología , Masculino , Persona de Mediana Edad , Tiempo de Protrombina , Factores de Riesgo , Factores Sexuales , Accidente Cerebrovascular/etiología , Accidente Cerebrovascular/patología , Trombofilia/complicaciones , Trombofilia/patología , Ucrania , Proteína Gla de la MatrizRESUMEN
Matrix Gla protein (MGP) gene allelic polymorphism in 170 patients with ischemic atherothrombotic stroke (IATS) and in 124 healthy people was determined. It was shown that in patients with IATS interrelation of main homozygotes, heterozygotes and minor homozygotes is 61,2, 31,2, 7,6% for T-138 --> C (rs1800802) promoter polymorphism (in control -59.7, 35.6, 4.8%, P = 0.521 by chi2-test); 35.9, 48.8, 15.3% for G(-7) --> A (rs1800801) polymorphism of promotor (in control--43.5, 50.0, 6.5%, P = 0.051); 39.4, 48.8, 11.8% for Thr83 --> Ala (rs4236) polymorphism of 4 exon (in control - 34.7, 53.2, 12.1%, P = 0.701). Significant differences in the frequency of G(-7) --> A polymorphism between patients with IATS and control group were revealed only for women (P = 0.022). The results obtained suggest that the A/A-variant of MGP gene promotor (G(-7) --> A polymorphism) is associated with an increased risk of IATS in female persons in the Ukrainian population.
Asunto(s)
Isquemia Encefálica/genética , Proteínas de Unión al Calcio/genética , Proteínas de la Matriz Extracelular/genética , Placa Aterosclerótica/genética , Polimorfismo de Nucleótido Simple , Accidente Cerebrovascular/genética , Población Blanca/genética , Adulto , Anciano , Anciano de 80 o más Años , Alelos , Isquemia Encefálica/complicaciones , Estudios de Casos y Controles , Exones , Femenino , Frecuencia de los Genes , Humanos , Masculino , Persona de Mediana Edad , Placa Aterosclerótica/complicaciones , Reacción en Cadena de la Polimerasa , Regiones Promotoras Genéticas , Factores de Riesgo , Factores Sexuales , Accidente Cerebrovascular/complicaciones , Ucrania , Proteína Gla de la MatrizRESUMEN
We showed here that energy metabolism in thoracic rabbit aorta and posterior vena cava is disturbed two weeks following the induction of alloxan-induced diabetes and monoiodacetate intoxication. In the vessels studied, the alterations are manifested in the decrease in the intensity of glucose uptake, oxygen consumption, lactic acid production, and ATP resynthesis. Simultaneously, the ATP content is significantly reduced. The possible significance of these disorders in the development of Monckeberg's arteriosclerosis is discussed.
Asunto(s)
Aorta Torácica/metabolismo , Diabetes Mellitus Experimental/metabolismo , Metabolismo Energético/efectos de los fármacos , Ácido Yodoacético/toxicidad , Venas Cavas/metabolismo , Adenosina Trifosfato/metabolismo , Aloxano , Animales , Aorta Torácica/efectos de los fármacos , Diabetes Mellitus Experimental/fisiopatología , Glucosa/metabolismo , Ácido Láctico/metabolismo , Masculino , Esclerosis Calcificante de la Media de Monckeberg/metabolismo , Consumo de Oxígeno/efectos de los fármacos , Conejos , Venas Cavas/efectos de los fármacosRESUMEN
The intensity of the lipid peroxydation (LPO) and the antioxidant enzyme activity (gluthathione peroxydase, superoxide dismutase and catalase) after monoiodacetate injection (10 mg/ kg) within 3, 7 and 14 days was examined in the arterial and venous walls of rabbits. The increase in the amount of the intermediate and final LPO products, and also the reduction in the antioxidant enzymes activity has been found in the vessels of all types. The changes in the studied parameters were more expressed in the venous vessels, than in the arteries. It is supposed that the activation of LPO is caused by the primary depression of antioxidant systems which arises as the consequence of the disorders of energy exchange in the vascular wall under the influence of monoiodacetate.
