Movement behaviour typologies and their associations with adiposity indicators in children and adolescents: a latent profile analysis of 24-h compositional data.

OBJECTIVES: Growing evidence supports the important role of 24-hour movement behaviours (MB) in preventing childhood obesity. However, research to understand the heterogeneity and variability of MB among individuals and what kind of typologies of individuals are at risk of developing obesity is lacking. To bridge this gap, this study identified typologies of 24-hour MB in children and adolescents and investigated their associations with adiposity indicators. METHODS: In this cross-sectional study, 374 children and 317 adolescents from the Czech Republic wore wrist-worn accelerometers for seven consecutive days. Time spent in moderate-to-vigorous physical activity (MVPA), light physical activity (LPA), sedentary behaviour (SB), and sleep was quantified using raw accelerometery data. Adiposity indicators included body mass index (BMI) z-score, fat mass percentage (FM%), fat mass index (FMI), and visceral adipose tissue (VAT). Bias-adjusted latent profile analysis was used on the 24-hour MB data to identify MB typologies and their associations with adiposity indicators. The models were adjusted for potential confounders. The identified typologies were labelled to reflect the behavioural profiles of bees to aid interpretability for the general public. RESULTS: Two typologies were identified in children: highly active Workers characterised by high levels of MVPA and LPA, and inactive Queens characterised by low levels of MVPA and LPA, high levels of SB and longer sleep duration compared to Workers. In adolescents, an additional typology labelled as Drones was characterised by median levels of MVPA, LPA, SB and longest sleep duration. After controlling for covariates, we found that children labelled as Queens were associated with 1.38 times higher FM%, 1.43 times higher FMI, and 1.67 times higher VAT than Workers. In adolescents, Drones had 1.14 times higher FM% and Queens had 1.36 higher VAT in comparison with Workers, respectively. CONCLUSION: Our study highlights the importance of promoting active lifestyles in children and adolescents to potentially reduce adiposity. These findings can provide insights for interventions aimed at promoting healthy MB and preventing childhood obesity.

The association between device-measured sitting time and cardiometabolic health risk factors in children.

BACKGROUND: There is limited evidence of the associations between postural-derived sitting time, waist-worn derived sedentary time and children's health and the moderation effect of physical activity (PA). This study examined associations of children's device-measured sitting time with cardiometabolic health risk factors, including moderation by physical activity. METHODS: Cross-sectional baseline data from children (mean-age 8.2 ± 0.5 years) in Melbourne, Australia (2010) participating in the TransformUs program were used. Children simultaneously wore an activPAL to assess sitting time and an ActiGraph GT3X to assess sedentary time and physical activity intensity. Cardiometabolic health risk factors included: adiposity (body mass index [BMI], waist circumference [WC]), systolic and diastolic blood pressure (SBP, DBP), high-density lipoprotein (HDL), low-density lipoprotein (LDL), cholesterol, triglycerides, fasting plasma glucose (FPG), serum insulin, and 25-hydroxyvitaminD (25[OH]D). Linear regression models (n = 71-113) assessed associations between sitting time with each health risk factor, adjusted for different PA intensities (i.e. light [LIPA], moderate-vigorous intensities [MVPA], separately on each model), age, sex, adiposity, and clustering by school. Interaction terms examined moderation. The analyses were repeated using device-measured sedentary time (i.e. ActiGraph GT3X) for comparison. RESULTS: Sitting time was positively associated with SBP (b = 0.015; 95%CI: 0.004, 0.026), DBP (b = 0.012; 95%CI:0.004, 0.020), and FPG (b = 0.001; 95%CI: 0.000, 0.000), after adjusting for higher PA intensities. The association between sitting time and insulin (b = 0.003; 95%CI: 0.000, 0.006) was attenuated after adjusting for higher PA intensities. When the models were adjusted for LIPA and MVPA, there was a negative association with LDL (b=-0.001; 95%CI: -0.002, -0.000 and b=-0.001; 95%CI: -0.003, -0.000, respectively). There was a negative association of sedentary time with WCz (b=-0.003; 95%CI: -0.005, 0.000) and BMIz (b=-0.003; 95%CI: -0.006, -0.000) when the models were adjusted by MVPA. Sedentary time was positively associated with triglycerides (b = 0.001; 95%CI: 0.000, 0.001) but attenuated after adjusting for MVPA. No evidence of moderation effects was found. CONCLUSIONS: Higher volumes of sitting and sedentary time were associated with some adverse associations on some cardiometabolic health risk factors in children. These associations were more evident when sitting time was the predictor. This suggests that reducing time spent sitting may benefit some cardiometabolic health outcomes, but future experimental research is needed to confirm causal relationships and identify the biological mechanisms that might be involved. TRIAL REGISTRATION: Australian New Zealand Clinical Trials Registry: ACTRN12609000715279.

Effectiveness of Intervention Strategies to Increase Adolescents' Physical Activity and Reduce Sedentary Time in Secondary School Settings, Including Factors Related to Implementation: A Systematic Review and Meta-Analysis.

BACKGROUND: Globally, just one in five adolescents meet physical activity guidelines and three-quarters of the school day is spent sitting. It is unclear which types of school-based interventions strategies increase physical activity and reduce sedentary time among adolescents, or how these interventions are implemented influences their effectiveness. OBJECTIVE: The three aims of our systematic review were to (a) identify intervention strategies used within secondary school settings to improve students' movement behaviours throughout school-based initiatives, delivered at or by the school; (b) determine the overall effect of the interventions (meta-analysis) on physical activity (all intensities), sedentary time, cognitive/academic, physical health and/or psychological outcomes; and (c) describe factors related to intervention implementation. METHODS: Searches were conducted in MEDLINE complete, EMBASE, CINAHL, SPORTDiscus, APA PsycINFO, and ERIC in January 2023 for studies that (a) included high school-aged adolescents; (b) involved a school-based intervention to increase physical activity and/or decrease sedentary time; and (c) were published in English. Reported effects were pooled in meta-analyses where sufficient data were obtained. RESULTS: Eighty-five articles, representing 61 interventions, met the inclusion criteria, with 23 unique intervention strategies used. Interventions that involved whole-school approaches (i.e., physical activity sessions, environmental modifications, teacher training, peer support and/or educational resources) were favourably associated with most of the outcomes. The meta-analyses showed: (a) non-significant effects for sedentary time (Standardized mean difference [SMD] = -0.02; 95%CI, -0.14, 0.11), physical activity at all intensities (light: SMD= -0.01; 95%CI, -0.08, 0.05; moderate: SMD = 0.06; 95%CI, -0.09, 0.22; vigorous: SMD = 0.08; 95%CI, -0.02, 0.18; moderate-to-vigorous: SMD = 0.05; 95%CI, -0.01, 0.12) and waist circumference (SMD = 0.09; 95%CI, -0.03, 0.21), and (b) a small statistically significant decrease in body mass index (SMD= -0.09, 95%CI -0.16, -0.0). Factors related to intervention implementation were reported in 51% of the articles. CONCLUSION: While some intervention approaches demonstrated promise, small or null effects were found in meta-analyses. Future school-based interventions should utilize a whole-school approach designed to increase adolescents' activity across the day. Consistent reporting of implementation will increase understanding of how interventions are adopted, implemented and sustained. REGISTRATION: PROSPERO (CRD42020169988).

The Arabidopsis DNA glycosylase MBD4L repairs the nuclear genome in vivo.

DNA glycosylases remove mispaired or modified bases from DNA initiating the base excision repair (BER) pathway. The DNA glycosylase MBD4 (methyl-CpG-binding domain protein 4) has been functionally characterized in mammals, but not yet in plants, where it is called MBD4-like (MBD4L). Mammalian MBD4 and Arabidopsis recombinant MBD4L excise U and T mispaired with G, as well as 5-fluorouracil (5-FU) and 5-bromouracil (5-BrU) in vitro. Here, we investigate the ability of Arabidopsis MBD4L to remove some of these substrates from the nuclear genome in vivo in coordination with uracil DNA glycosylase (AtUNG). We found that mbd4l mutants are hypersensitive to 5-FU and 5-BrU, as they displayed smaller size, less root growth, and higher cell death than control plants in both media. Using comet assays, we determined BER-associated DNA fragmentation in isolated nuclei and observed reduced DNA breaks in mbd4l plants under both conditions, but particularly with 5-BrU. The use of ung and ung x mbd4l mutants in these assays indicated that both MBD4L and AtUNG trigger nuclear DNA fragmentation in response to 5-FU. Consistently, we here report the nuclear localization of AtUNG based on the expression of AtUNG-GFP/RFP constructs in transgenic plants. Interestingly, MBD4L and AtUNG are transcriptionally coordinated but display not completely overlapping functions. MBD4L-deficient plants showed reduced expression of BER genes and enhanced expression of DNA damage response (DDR) gene markers. Overall, our findings indicate that Arabidopsis MBD4L is critical for maintaining nuclear genome integrity and preventing cell death under genotoxic stress conditions.

The impact of height-adjustable desks and classroom prompts on classroom sitting time, social, and motivational factors among adolescents.

PURPOSE: This quasi-experimental study examined the impact of height-adjustable desks in combination with prompts to break up prolonged sitting time during class time and identified social and motivational factors associated with breaking up sitting time among adolescents. Teachers' perceptions of strategies were also examined. METHODS: Over 17 weeks, 1 classroom in a government secondary school in Melbourne, Australia, was equipped with 27 height-adjustable desks and prompts (posters and desk stickers) to break up classroom sitting time. Teachers received professional development in the use of the desks and prompts. One group of adolescents (n = 55) had 2-5 lessons/week using the height-adjustable desks in an intervention classroom, and a comparison group matched by year level and subject (n = 50) was taught in traditional "seated" classrooms. Adolescents wore an activPAL monitor at baseline (T0), 4 weeks (T1), and 17 weeks (T2) and completed a survey at T0 and T2. Six teachers participated in interviews at T2. Effect sizes were calculated (d). RESULTS: Linear mixed models found that, compared to the traditional "seated" classrooms, the adolescents in the intervention classroom had significantly lower sitting time (T1: -9.7 min/lesson, d = -0.96; T2: -6.7 min/lesson, d = -0.70) and time spent in sitting bouts >15 min (T2: -11.2 min/lesson, d = -0.62), and had significantly higher standing time (T1: 7.3 min/lesson, d = 0.84; T2: 5.8 min/lesson, d = 0.91), number of breaks from sitting (T1: 1.3 breaks/lesson, d = 0.49; T2: 1.8 breaks/lesson, d = 0.67), and stepping time (T1: 2.5 min/lesson, d = 0.66). Intervention classroom adolescents reported greater habit strength (d = 0.58), self-efficacy for breaking up sitting time (d = 0.75), and indicated that having a teacher/classmate remind them to stand as helpful (d = 0.50). CONCLUSION: This intervention shows promise for targeting sitting behaviors in the classroom and indicates that incorporating social and motivational strategies may further enhance outcomes.

"Everything kind of revolves around technology": a qualitative exploration of families' screen use experiences, and intervention suggestions.

BACKGROUND: Managing children's screen time is challenging for most families. Interventions have had limited success in reducing screen time, potentially due to a lack of understanding of the experiences, needs and recommendations of families. This study aimed to 1) understand the screen time experiences of families, particularly during COVID-19 lockdowns; and 2) explore parent and child suggestions for the design, components, and content of a screen time management program. METHODS: Parents and children from 30 families living in Victoria, Australia completed a semi-structured interview (63 interviews) via Zoom in October-November 2021. Parents were maged 40.8 (± 8.9) years and predominantly female (90%). Children were maged 11.4 (± 2.4) years and 47% female. The interviews were audio recorded, transcribed verbatim and analysed using inductive thematic analysis combined with a summative content analysis approach. RESULTS: Three themes under Aim 1 emerged. Theme 1) 'Screen time management experiences and practices', including rules and strategies, challenges, and the impact of COVID-19 lockdowns. Theme 2) 'Impact of screens on family interaction and communication' including conflicts within the family, reduced face-to-face interactions, and negative impact on child's behaviour and wellbeing. Theme 3) 'Benefits of increased screen time due to COVID-19 lockdowns' including continuation of social interactions, extracurricular activities, improved technology skills and using screens as a 'babysitter'. Findings from Aim 2 suggest that families want a screen time management program delivered online to parents and children, which includes static and interactive content that incorporates health information, alternative activities, cyber-safety information, tips for goal setting and rewards, screen monitoring tools, links to reputable information, and parent social connections. Reminders via text message or through the online platform would help maintain engagement in the program. CONCLUSIONS: Families are experiencing challenges in managing the complex balance between the increased need for screens and the impact it has on the family. These findings provide valuable parent and child insights to assist in developing screen time management programs that are created with an understanding of the needs and challenges of families.

Understanding Australian adolescent girls' use of digital technologies for healthy lifestyle purposes: a mixed-methods study.

BACKGROUND: Little is known about girls' use of a broad range of digital technologies to support a healthy lifestyle, particularly during the later adolescent years when they are expected to take greater responsibility for individually managing their health and wellbeing. The present study was designed to gain an in-depth understanding of adolescent girls', 15 to 17 years of age, use of a broad range of digital technologies to support multiple healthy lifestyle purposes. METHODS: This study used a mixed-methods sequential research design (i.e. quantitative and qualitative data in two consecutive phases). A quantitative online survey was conducted to determine the use of digital technologies for healthy lifestyle purposes. Qualitative semi-structured interviews were conducted with a subset of survey respondents to explore factors influencing their technology use and preferences. RESULTS: Descriptive analysis of survey data (online survey, n = 336) demonstrated the popularity of social media and online video sharing platforms, with evidence that the use of digital technologies does not occur in isolation and girls draw on several resources simultaneously to achieve their desired healthy lifestyle outcomes. Thematic analysis of interview data (semi-structured interviews, n = 29) revealed seven interrelated themes around factors influencing girls' use of, and satisfaction with, different digital technologies: accessibility, social connectivity, credibility, relatability, inspiration, safety, and customisability. CONCLUSIONS: The findings reiterate that one size doesn't fit all girls, and often one size might not even fit one girl, and thus highlight the challenge of catering to such varied use cases. Overall, it appears that current digital technology offerings meet the needs and preferences of adolescent girls for healthy lifestyle purposes, however there may be benefit in allowing greater customisation of use, and consumer driven tailoring of content, according to personal preferences and changing circumstances.

Use of a nutrition-focused quality improvement program for community-living older adults at malnutrition risk is associated with better nutritional outcomes.

BACKGROUND & AIMS: Among older adults, malnutrition or its risk is common and is associated with increased morbidity and mortality plus increased need for healthcare utilization. We aimed to identify and treat malnutrition risk among older adults who received care at an outpatient clinic after a recent hospitalization and/or for management of a chronic disease. METHODS: From the outpatient clinic of Hospital Universitario San Ignacio, Bogotá, Colombia, we recruited older adults (>60 years) with malnutrition or its risk according to the Mini Nutritional Assessment-Short Form (MNA-SF). Patients were excluded if they had dementia or were not expected to live 90 days or more. Intervention was a nutrition-focused quality improvement program (QIP) including: i) education of patients and caregivers about the health importance of complete and balanced macro- and micronutrient intake plus physical exercise; and ii) nutritional intervention with dietary counseling and provision of oral nutritional supplements (ONS) for daily intake. To assess the effect of our intervention, we collected nutritional outcome data pre- and post-participation of patients in the nutrition-focused QIP. For pre-post comparisons, we used MNA-SF scores and calf circumference (a proxy for leg muscle mass) measures along with nutrition-related anthropometric determinations of body weight and body mass index (BMI). The ONS treatment phase was 60 days, with follow-up measurements up to 30 days after ONS treatment ended (90 days after intervention start). RESULTS: Of 677 enrolled patients, 618 completed the QIP, while 565 had complete anthropometric data. Patients had a mean age of 74.1 ± 8.7 years, an average of 2.6 comorbidities, included a high proportion of females (69.4%), with medium socioeconomic status (76%). After QIP intervention, 324 (52.4%) patients had improvement in nutritional outcomes; improvement was significant in all four measures (P-values < 0.001). Higher ONS adherence was associated with the highest improvement in nutritional status. CONCLUSIONS: For community-living older adults receiving outpatient care, comprehensive nutritional care offered via a nutrition-focused QIP was associated with significant improvements in indicators of nutritional status (MNA-SF scores and calf circumference) and maintenance or improvements in nutrition-related anthropometric measures (body weight and BMI). GOV IDENTIFIER: NCT04042987.

Does light-intensity physical activity moderate the relationship between sitting time and adiposity markers in adolescents?

BACKGROUND: While the relationship between sedentary time and adiposity markers may be independent of moderate-to-vigorous intensity physical activity (MVPA) among adolescents, little is known about the role of light-intensity physical activity (LIPA) in this relationship. The aim of this cross-sectional study was to examine whether device-measured LIPA and MVPA moderate the associations between objectively measured sitting time and adiposity markers (body mass index (BMI)) and waist circumference (WC)) among adolescents. METHODS: This study included accelerometer and inclinometer data obtained from 219 adolescents (age = 14.9 ± 1.6 years, mean ± SD), collected during 2014 and 2015 in Melbourne, Australia. ActiGraph GT3X accelerometers were used to determine time spent in total-LIPA (101 counts/min to 3.99 metabolic equivalents (METs)) was dichotomized into low-LIPA (101-799 counts/min) and high LIPA (800 counts/min to 3.99 METs), and MVPA (≥ 4 METs). The average time spent sitting was obtained from activPAL inclinometers. Anthropometric measures were assessed by trained staff. Interactions between sitting and total-LIPA, low-LIPA, high-LIPA, and MVPA on BMI z-score (zBMI) and WC z-score (zWC), respectively, were examined using linear regression, adjusting for age and sex; and moderation by total-LIPA, low-LIPA, high-LIPA, and MVPA were examined by adding interaction terms. Significant interaction effects were probed by comparing associations at the mean and at 1 SD below and above the mean. RESULTS: Total-LIPA significantly moderated the association between sitting time and zBMI, and low-LIPA significantly moderated the association between sitting time and zBMI and zWC. No other associations were found for total-LIPA, high-LIPA, or MVPA. Specifically, at high levels of total-LIPA (+1 SD), there is a negative association between sitting time and zBMI. In addition, at high levels of low-LIPA (+1 SD), there is a negative association between sitting time and zBMI and zWC. CONCLUSION: Associations between sitting and adiposity depended on time spent in total-LIPA and low-LIPA, but not high-LIPA or MVPA. Results suggest that increasing time spent in LIPA may provide protection from the deleterious effects of sitting on adiposity markers among adolescents. Experimental evidence is needed to support these conclusions.

Urban Populations of Aedes aegypti (Diptera: Culicidae) From Central Argentina: Dispersal Patterns Assessed by Bayesian and Multivariate Methods.

Aedes aegypti (L.), the main vector of dengue and other arboviruses, was declared eradicated from Argentina in 1964; however, in 1987, it was detected again and nowadays it occurs in most of the country territory. To understand the transmission of vector-borne diseases, knowledge of the dispersal of vector populations is essential to evaluate the risk of pathogen transmission. We conducted a population genetic analysis of Ae. aegypti in 20 neighborhoods from Córdoba, the second largest city in Argentina, using 10 microsatellite loci. High genetic differentiation and the absence of an isolation by distance pattern was found using Weir and Cockerham's θ. Bayesian and multivariate clustering analyses showed that the studied sites included individuals with high membership coefficients (Q) in their populations, individuals with membership in another cluster, and admixed individuals. Individuals with high Q in clusters different from the population in which they were collected strongly suggests that passive transport is important in shaping the Ae. aegypti dispersal pattern in Córdoba city. Knowing the genetic structure of Ae. aegypti populations and their dispersal patterns would contribute to the implementation of vector control programs.

Adoption, implementation and sustainability of school-based physical activity and sedentary behaviour interventions in real-world settings: a systematic review.

BACKGROUND: Globally, many children fail to meet the World Health Organization's physical activity and sedentary behaviour guidelines. Schools are an ideal setting to intervene, yet despite many interventions in this setting, success when delivered under real-world conditions or at scale is limited. This systematic review aims to i) identify which implementation models are used in school-based physical activity effectiveness, dissemination, and/or implementation trials, and ii) identify factors associated with the adoption, implementation and sustainability of school-based physical activity interventions in real-world settings. METHODS: The review followed PRISMA guidelines and included a systematic search of seven databases from January 1st, 2000 to July 31st, 2018: MEDLINE, EMBASE, CINAHL, SPORTDiscus, PsycINFO, CENTRAL, and ERIC. A forward citation search of included studies using Google Scholar was performed on the 21st of January 2019 including articles published until the end of 2018. Study inclusion criteria: (i) a primary outcome to increase physical activity and/or decrease sedentary behaviour among school-aged children and/or adolescents; (ii) intervention delivery within school settings, (iii) use of implementation models to plan or interpret study results; and (iv) interventions delivered under real-world conditions. EXCLUSION CRITERIA: (i) efficacy trials; (ii) studies applying or testing school-based physical activity policies, and; (iii) studies targeting special schools or pre-school and/or kindergarten aged children. RESULTS: 27 papers comprising 17 unique interventions were included. Fourteen implementation models (e.g., RE-AIM, Rogers' Diffusion of Innovations, Precede Proceed model), were applied across 27 papers. Implementation models were mostly used to interpret results (n = 9), for planning evaluation and interpreting results (n = 8), for planning evaluation (n = 6), for intervention design (n = 4), or for a combination of designing the intervention and interpreting results (n = 3). We identified 269 factors related to barriers (n = 93) and facilitators (n = 176) for the adoption (n = 7 studies), implementation (n = 14 studies) and sustainability (n = 7 studies) of interventions. CONCLUSIONS: Implementation model use was predominately centered on the interpretation of results and analyses, with few examples of use across all study phases as a planning tool and to understand results. This lack of implementation models applied may explain the limited success of interventions when delivered under real-world conditions or at scale. TRIAL REGISTRATION: PROSPERO (CRD42018099836).

Exploring when and how adolescents sit: cross-sectional analysis of activPAL-measured patterns of daily sitting time, bouts and breaks.

BACKGROUND: This study describes patterns of adolescents' objectively-measured sitting volume, sitting bouts, and breaks in sitting during different days and periods of the day, and explored differences by sex and weekdays versus weekend days. METHODS: ActivPAL™ data were collected in August 2014-December 2015 from adolescents attending secondary government schools in Melbourne Australia. Eight periods (early morning, mid-morning, morning break, late morning, lunch, early afternoon, late-afternoon and evening) were extracted for each day. School time, class time and out-of-school time were also extracted for weekdays. The percentage of time sitting, percentage of each hour in prolonged sitting (sitting bout ≥10 min), and number of sitting breaks/hour were calculated for each period. Differences by sex, and week and weekend days were determined using t-tests. RESULTS: Participants (n = 297, 15.4 ± 1.6 years) spent 68% of their day sitting; ~ 30% of each hour in prolonged sitting and 3.1 sitting breaks/hour. Sitting time was greater during class time (75%) and school (70%) compared to out-of-school time (65%). Sitting patterns differed between week and weekend days for all periods except the evening period. Girls had higher proportion of sitting during class than boys (76% vs 72% respectively) and school hours (72% vs 67%), more prolonged sitting during school hours (27% vs 23%), and more sitting breaks per hour during out-of-school time (2.6 vs 2.4), but fewer during class (2.5 vs 3.3) and school hours (2.7 vs 3.3). Sitting patterns did not differ by sex on weekend days. CONCLUSIONS: Adolescents spent two-thirds of their waking hours sitting, with distinct patterns on weekdays and weekend days. Even though boys and girls were exposed to the same school day routine, girls spent more time sitting and had fewer sitting breaks. Class times, school breaks and the evening period were identified as key intervention periods. Further research is needed to understand the behavioural differences, and guide future intervention design.

Longitudinal Changes in Sitting Patterns, Physical Activity, and Health Outcomes in Adolescents.

This study examined two-year changes in patterns of activity and associations with body mass index (BMI) and waist circumference (WC) among adolescents. Inclinometers (activPAL) assessed sitting, sitting bouts, standing, stepping, and breaks from sitting. ActiGraph-accelerometers assessed sedentary time (SED), light-intensity physical activity (LIPA, stratified as low- and high-LIPA), and moderate-to-vigorous physical activity (MVPA). Anthropometric measures were objectively assessed at baseline and self-reported at follow-up. Data from 324 and 67 participants were obtained at baseline and follow-up, respectively. Multilevel mixed-effects linear regression models examined changes over time, and associations between baseline values and BMI and WC at follow-up. There were significant increases in BMI (0.6 kg/m²) and durations of prolonged sitting (26.4 min/day) and SED (52 min/day), and significant decreases in stepping (-19 min/day), LIPA (-33 min/day), low-LIPA (-26 min/day), high-LIPA (-6.3 min/day), MVPA (-19 min/day), and the number of breaks/day (-8). High baseline sitting time was associated (p = 0.086) with higher BMI at follow-up. There were no significant associations between baseline sitting, prolonged sitting, LIPA, or MVPA with WC. Although changes in daily activity patterns were not in a favourable direction, there were no clear associations with BMI or WC. Research with larger sample sizes and more time points is needed.

The impact of height-adjustable desks and prompts to break-up classroom sitting on adolescents' energy expenditure, adiposity markers and perceived musculoskeletal discomfort.

Adolescents spend large amounts of time sitting at school. Little is known about the impact of reducing and breaking-up prolonged sitting during school lessons on adolescents' health. This study aimed to investigate the impact of an intervention to reduce classroom sitting time on adolescents' energy expenditure (EE; kcal/lesson), body mass index (BMI), waist circumference (WC), and musculoskeletal discomfort. A secondary school classroom was equipped with height-adjustable desks, posters promoting the health benefits of and strategies for breaking-up sitting time, and desk stickers reminding students to periodically stand up. Classroom teachers participated in a professional development session. Using a quasi-experimental design, differences between 49 participants who utilised the intervention classroom 2-5 times/week and a comparison group (39 adolescents, matched by year level and subject) who used traditional classrooms, were examined. EE, BMI and WC were objectively measured and musculoskeletal discomfort was self-reported at baseline, 4-weeks, and 17-weeks. Hierarchical linear and multilevel logistic regression-mixed models were used to examine intervention effects, adjusting for baseline values, sex and age. EE was significantly higher at 4-weeks and 17-weeks (29.4 and 37.7 kcal/lesson, respectively), BMI was higher at 4-weeks (0.34 kg/m2), and WC was lower at 4-weeks and 17-weeks (-3.53 and -2.64 cm, respectively) in the intervention compared to the comparison group. No intervention effect was found for musculoskeletal discomfort. Findings provide preliminary indications that these strategies may benefit health among adolescents in the short term. However, extended longer-duration trials are needed to determine longer-term health effects.

Impact of an 8-Month Trial Using Height-Adjustable Desks on Children's Classroom Sitting Patterns and Markers of Cardio-Metabolic and Musculoskeletal Health.

During school hours, children can sit for prolonged and unbroken periods of time. This study investigated the impact of an 8-month classroom-based intervention focusing on reducing and breaking-up sitting time on children's cardio-metabolic risk factors (i.e., body mass index, waist circumference, blood pressure) and perceptions of musculoskeletal discomfort. Two Year-6 classes (24 students per class) in one primary school were assigned to either an intervention or control classroom. The intervention classroom was equipped with height-adjustable desks and the teacher was instructed in the delivery of pedagogical strategies to reduce and break-up sitting in class. The control classroom followed standard practice using traditional furniture. At baseline, and after 8-months, time spent sitting, standing, stepping, and sitting-bouts (occasions of continuous sitting) as well as the frequency of sit-to-stand transitions were obtained from activPAL inclinometers and the time spent in light-intensity physical activity was obtained from ActiGraph accelerometers. Demographics and musculoskeletal characteristics were obtained from a self-report survey. Hierarchical linear mixed models found that during class-time, children's overall time spent sitting in long bouts (>10 min) were lower and the number of sit-to-stand transitions were higher in the intervention group compared to the control group, while no changes were observed for musculoskeletal pain/discomfort. No significant intervention effects were found for the anthropometrics measures and blood pressure. Height-adjustable desks and pedagogical strategies to reduce/break-up sitting can positively modify classroom sitting patterns in children. Longer interventions, larger and varied sample size may be needed to show health impacts; however, these desks did not increase musculoskeletal pain/discomfort.

Validity of self-measured waist circumference in adults at risk of type 2 diabetes and cardiovascular disease.

BACKGROUND: Waist circumference (WC) is used to indirectly measure abdominal adipose tissue and the associated risk of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). Because of its easy implementation and low cost, self-measured WC is commonly used as a screening tool. However, discrepancies between self-measured and objectively measured WC may result in misclassification of individuals when using established cut-off values. The aim of this study was to determine the accuracy of self-measured WC in adults at risk of T2DM and/or CVD, and to determine the anthropometric, demographic and behavioural characteristics associated with bias in self-measured WC. METHODS: Self-measured and objectively measured WC was obtained from 622 participants (58.4% female; mean age 43.4 ± 5.3 years) in the Hoorn Prevention Study. The associations of gender, age, educational level, body mass index, smoking status, dietary habits, physical activity and sedentary behaviour with the discrepancies between self-measured and objectively measured WC were analysed using independents t-test and one-way ANOVA. Bland-Altman plots were used to plot the agreement between the two measures. RESULTS: On average, self-measured WC was overestimated by 5.98 ± 4.82 cm (P < 0.001). Overestimation was consistent across all subgroups, but was more pronounced in those who were younger and those with lower educational attainment. CONCLUSIONS: The results support self-measured WC as a useful tool for large-scale populations and epidemiological studies when objective measurement is not feasible, but overestimation should be taken into account when screening adults at risk of T2DM and/or CVD.

Neoplasia intraepitelial anal / Anal intraepithelial neoplasia

RESUMEN Introducción: el virus del papiloma humano se asocia a las neoplasias cervicales y epiteliales anales. Objetivos: determinar la prevalencia de neoplasia intraepitelial anal (NIA) y las características clínicas en mujeres que acuden al consultorio de patología cervical en el Hospital Regional de San Lorenzo. Materiales y Métodos: estudio observacional, descriptivo, prospectivo realizado en 900 mujeres que acudieron al consultorio de patología cervical en el Hospital Regional de San Lorenzo, en los años 2012-2013 mediante colposcopía y citología anal. Resultados: Se encontraron 60 casos (8,5%) de NIA. Las características de las pacientes con NIA fueron: grupo etario predominante 19 a 25 años, 50% de práctica de relaciones sexuales anales, 25% con tabaquismo, inicio de las relaciones sexuales antes de los 16 años (70%), 2 a 4 parejas sexuales (81,6%). El riesgo de NIA en pacientes con hábitos sexuales anales fue significativo: OR 1,91 (IC95% 1,09-3,36) (p 0,01). Conclusiones: la prevalencia de NIA en mujeres fue 8,5%. Se halló asociación significativa con hábito de relaciones sexuales anales.

ABSTRACT Introduction: Human papillomavirus associated with cervical and anal epithelial neoplasia. Objectives: To determine the prevalence clinic of anal intraepithelial neoplasia (AIN) and the clinical characteristics in women attending the out patient of cervical pathology in the Regional Hospital of San Lorenzo. Material and Methods: An observational, descriptive, prospective study of 900 women attended the out patient clinic of cervical pathology in the Regional Hospital of San Lorenzo, in the years 2012-2013 through anal cytology and colposcopy. Results: 60 cases (8.5%) of NIA where found. The characteristics of the patients with NIA were: predominant age group 19-25 years, 50% of them sexual practicing anal intercourse, 25% smoking, initiation of sexual intercourse before 16 year of age (70%), 2-4 sexual pairs (81.6%). NIA risk in patients with anal sexual habits were significant: OR 1.91 (95% CI 1.09 to 3.36) (p 0.01). Conclusions: The prevalence of AIN in women was 8.5%. Significant association was found with anal sexual intercourse.

Frecuencia de candidiasis vaginal en embarazadas del Hospital Nacional de Itauguá / Vaginal frecuency of candidiasis in pregnancy women of the Hospital Nacional of Itauguá

Introducción: la frecuencia de infección vaginal por Candida spp. durante el embarazo y su probable repercusión varían con cada población. Objetivo: conocer la frecuencia de candidiasis vaginal en embarazadas que acuden a la consulta hospitalaria y su sensibilidad con los antifundicos. Metodología: estudio observacional prospectivo descriptivo, que incluyó embarazadas que acudieron al consultorio de prenatal en el Hospital Nacional (Itauguá, Paraguay) desde marzo a julio del 2008. Resultados: se estudiaron 91 embarazadas, siendo la edad media de 25 años (15 - 44 años) con una desviación estándar de 6,9 años. En 40 de 91 muestras estudiadas (44 %) se encontró flora alterada y en 51 muestras con flora habitual. Candida spp. en 20 de las 40 muestras (21 %); donde Candida albicans 14 (70 %), C. glabata 4 (20 %), C. tropicalis 1 (5 %), C. krusei 1 (5 %). Conclusión: se aislaron levaduras e 21 % de las muestras, siendo en su mayoría Candida albicans, y con más frecuencia en las que cursaban el tercer trimestre gestacional. Se encontró amplia sensibilidad a los antifúngicos y resistencia en las que se aislaron C. glabrata.

Introduction: the frequency of vaginal infection by Candida spp. during pregnancy and its probable repercussion with each population. Objective: to know the frequency of vaginal candidiasis in pregnant women who come to the hospital consultation and their sensitivity with antifundicos. Methodology: descriptive prospective observational study, which included pregnant women who attended the prenatal clinic at the Hospital Nacional (Itauguá, Paraguay) from March to July 2008. Results: 91 pregnant women were studied, with a mean age of 25 years (15 - 44 years) with a standard deviation of 6.9 years. Altered flora was found in 40 of 91 samples studied (44%) and in 51 samples with habitual flora. Candida spp. in 20 of the 40 samples (21%); where Candida albicans 14 (70%), C. glabata 4 (20%), C. tropicalis 1 (5%), C. krusei 1 (5%). Conclusion: yeasts were isolated in 21% of the samples, the majority being Candida albicans, and more frequently in those in the third trimester of pregnancy. Wide sensitivity to antifungals and resistance were found in which C. glabrata was isolated.

Diagnóstico de cáncer de cuello uterino y lesiones precursora a través de la inspección visual secundaria a la aplicación del ácido acético al 5 porciento / Diagnostic of cervical cancer and early lesions thray a clinical visual examination of cervix using 5 porciento acetic acid

El objetivo del estudio es demostrar la sensibilidad, especificidad, de la prueba de inspección del ácido acético, correlacionándola con la citología oncológica para detectar lesiones precursoras y cáncer de cuello uterino. Se realizó un estudio analítico de prueba diagnóstica y se estudiaron 350 mujeres comprendidas entre 18 a 60 años de edad. La prueba de sensibilidad resulta de 93 pto y su especificidad de 97 pto y en la citología oncológica una sensibilidad de 42 pto y especificidad de 100 pto. Se hallaron 29 casos de lesiones de bajo grado (8pto), 15 casos de lesiones de alto grado (4pto) y 3 casos de cáncer (0.8pto). Los datos demostraron que el test de IVAA puede ser aceptado como método de detección para el cáncer cervica, especialmente en áreas de desprovistas de recursos, ya que demostró ser muy sensible y empleada como complemento a la citología oncológica que tiene alta especificidad.

Ruta diagnóstica y tratamiento del feocromocitoma. Presentación de dos casos clínicos / The treatment and diagnosis of pheochromocytoma. Report two cases

Se presentan dos casos de feocromocitoma. La sospecha se hace cuando presentan hipertensión arterial, sintomatología adrenérgica o hipotensión, el diagnóstico se realizó bajo sospecha clínica, bioquímicamente encontrando niveles de adrenalina y noradrenalina cuarenta y tres veces mayores a lo normal, la localización se realizó por ultrasonido, TAC, y rastreo con metayodobencilguanidina, los dos pacientes recibieron tratamiento médico a base de bloqueadores de canales de calcio, prazosín, Tratamiento definitivo resección quirúrgica, los dos pacientes actualmente se encuentran sanos sin tratamiento médico