RESUMEN
Chitosan-based biomaterials are being investigated for their unique properties that support skin regeneration and wound healing. This study focused on the preparation and characterization of a mupirocin (Mup)-loaded PEGylated chitosan (CS-PEG) nanoparticulate film (NF) [CBNF]. The CBNF was characterized using FTIR spectroscopy and SEM analysis. The results demonstrated that CBNF was successfully incorporated into the composites, as shown by functional group modification through FTIR analysis. Additionally, the SEM micrograph revealed the deposition of nanoparticles (<200 nm) on the surface of transparent CBNF. The film has higher water absorption (≥1700%) and moderate water retention ability within 6 h. Furthermore, histological findings showed significant development, with re-epithelialization and granulation of tissues after 19 days, indicating the healing efficiency of CNBF. These results suggest that drug-loaded films could be an effective carrier and delivery agent for Mup-like anti-inflammatory drugs.
Asunto(s)
Quitosano , Mupirocina , Nanopartículas , Polietilenglicoles , Cicatrización de Heridas , Quitosano/química , Mupirocina/química , Mupirocina/farmacología , Mupirocina/administración & dosificación , Cicatrización de Heridas/efectos de los fármacos , Nanopartículas/química , Polietilenglicoles/química , Animales , Portadores de Fármacos/química , Antibacterianos/química , Antibacterianos/farmacología , Antibacterianos/administración & dosificación , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The conventional production of chitosan from crustaceans has many limitations. An attempt was made to optimize chitosan production from fungi. Soil fungi were isolated, identified, and screened for high glucosamine content. Among the fungal isolates tested, Cunninghamella echinulata showed high glucosamine content. The biomass production of C. echinulata was standardized under different growth parameters. The physicochemical characterization of derived chitosan isolates was distinctive and diverged as supported by the FT-IR, molecular mass distribution, degree of deacetylation, and crystallinity. Molecular mass distribution ranged from 1 to 9 mers. The degree of deacetylation was observed to be maximum in C6 (80.88%), which increased with the increase in alkali concentration. In the chitosan isolate, C1 was non-toxic to Vero cells up to 250 µg/mL. In the physicochemical and functional properties of chitosan isolate, C1 was found to be unique and diverse; further detailed investigations on this isolate might help to develop some biomaterials with improved biocompatibility.
Asunto(s)
Quitosano , Cunninghamella , Animales , Chlorocebus aethiops , Quitosano/farmacología , Quitosano/química , Espectroscopía Infrarroja por Transformada de Fourier , Células VeroRESUMEN
Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV-Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs showed the maximum absorbance at 420-430 nm. The transmission electron micrograph revealed the formation of considerably uniform-sized AgNPs with an average size of 20-50 nm. The reducing and capping agents responsible for AgNP synthesis were identified by FT-IR. AgNP-incorporated cotton fabrics exhibited promising antibacterial activity against pathogenic bacteria. In addition, the in vitro cell viability of Vero cells (African green monkey kidney cells) was analyzed and the IC50 value of AgNPs was found to be 62.8 µg/mL. Taken together, these results clearly reveal less toxic AgNPs which could be exploited for various biomedical applications.
