1.
Methods Enzymol
; 181: 510-7, 1990.
Artículo
en Inglés
| MEDLINE
| ID: mdl-2199763
Asunto(s)
Endorribonucleasas/aislamiento & purificación , Oocitos/enzimología , Animales , Núcleo Celular/enzimología , Centrifugación por Gradiente de Densidad/métodos , Cromatografía en Gel/métodos , Cromatografía por Intercambio Iónico/métodos , Clonación Molecular , Detergentes , Endorribonucleasas/metabolismo , Femenino , Indicadores y Reactivos , Cinética , Octoxinol , Radioisótopos de Fósforo , Plásmidos , Polietilenglicoles , Pronasa , ARN de Transferencia de Leucina/genética , ARN de Transferencia de Leucina/metabolismo , Técnica de Dilución de Radioisótopos , Xenopus laevis
2.
Cell
; 55(4): 731-8, 1988 Nov 18.
Artículo
en Inglés
| MEDLINE
| ID: mdl-3180224
RESUMEN
To investigate the mechanism by which the purified Xenopus tRNA splicing endonuclease recognizes its splice sites, we utilized yeast pre-tRNA(3Leu) and pre-tRNA(Phe) variants constructed by in vitro mutagenesis. We found that the endonuclease interacts with conserved features of the mature tRNA domain. In particular, U8 and C56 may be examples of contact points between protein and RNA. Given that there are no conserved sequences at the splice junctions, the specificity of cutting at both splice sites is determined by the length of the anticodon stem. Although in general, the sequence of the intron is unimportant for splicing, there are some structural requirements.
