RESUMEN
The present paper describes the validation of a spectrophotometry method involving molecular absorption in the visible ultraviolet-visible (UV-Vis) region for selenium (Se) determination in the bacterial biomass produced by lactic acid bacteria (LAB). The method was found to be suitable for the target application and presented a linearity range from 0.025 to 0.250â¯mg/L Se. The angular and linear coefficients of the linear equation were 1.0678 and 0.0197â¯mg/L Se, respectively, and the linear correlation coefficient (R2) was 0.9991. Analyte recovery exceeded 96% with a relative standard deviation (RSD) below 3%. The Se contents in LAB ranged from 0.01 to 20â¯mg/g. The Se contents in the bacterial biomass determined by UV-Vis were not significantly different (pâ¯>â¯0.05) those determined by graphite furnace atomic absorption spectrometry. Thus, Se can be quantified in LAB biomass using this relatively simpler technique.
